FastQCFastQC Report
Sat 3 Sep 2022
EGAF00000654112

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000654112
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5159745
Sequences flagged as poor quality0
Sequence length100
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN370375771.78178378970279No Hit
NNNNNNNNNNNNNNNNNNNNNNNANNNNNNNNNNNNNNNNNNNNNNNNNN674511.3072545251751782No Hit
NNNNNNNNNNNNNNNNNNNNNNNTNNNNNNNNNNNNNNNNNNNNNNNNNN662411.2838037538676814No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNANNNNNNNNNNNNNNNNNNNN661711.2824470976763387No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNNNNNNNNNNNNNNNNNNNN607191.1767829611734688No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN477740.9258984697887203No Hit
NNNNNNNNNNNNNNNNNNNNNNNCNNNNNNNNNNNNNNNNNNNNNNNNNN450890.8738610144493574No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNNNNNNNNNNNNNNNNNNNN435520.8440727206480165No Hit
NNNNNNNNNNNNNNNNNNNNNNNGNNNNNNNNNNNNNNNNNNNNNNNNNN423260.8203118564967842No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNCNNNNNNNNNNNNNNNNNNNN419130.8123075849678618No Hit
NNNNNNNNNNNNNNNNNNNANNNNNNNNNNNNNNNNNNNNNNNNNNNNNN335420.6500708852859977No Hit
NNNNNNNNNNNNNNNNNANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN328630.6369113202299727No Hit
NNNNNNNNNANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN322570.6251665537734907No Hit
NNNNNNNNNNNNNNNNNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN316670.6137318801607444No Hit
NNNNNNNNNNNNNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN311280.6032856274874049No Hit
NNNNNNNNNNNNNANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN310390.6015607360441262No Hit
NNNNNNNNNNNNNNNNNNNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN302390.5860560938573515No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANNNN299410.5802806146427778No Hit
NNNNNNNNNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN296640.5749121322856071No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNNNN293920.5696405539421037No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANNNNNNNNNNNNNNNNNNN291500.5649503996806045No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNNNNNNNNNNNNNNNNNNN276210.5353171523011312No Hit
NNNNNNNNNNNNNNNNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN272830.5287664409772188No Hit
NNNNNNNNNNNNNNNNANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN272790.5286889177662849No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN267540.518513996331214No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNNNN242990.47093412562054904No Hit
NNNNNNNNNNNNNNNNNNNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN234750.4549643441681711No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN228650.4431420545007554No Hit
NNNNNNNNNNNNNNNNNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN226580.43913022833492743No Hit
NNNNNNNNNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN223070.43232756657548No Hit
NNNNNNNNNNNNNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN216010.41864471984565127No Hit
NNNNNNNNNNNNNNNNNCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN215180.4170361132187734No Hit
NNNNNNNNNNNNNNNNNNNCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN212140.411144349187799No Hit
NNNNNNNNNCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN209760.40653171813723354No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCNNNN203250.3939148155577456No Hit
NNNNNNNNNNNNNCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN197410.38259642676140004No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNNNNNNNNNNNNNNNNNNN182900.3544748819951374No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCNNNNNNNNNNNNNNNNNNN180940.3506762446593775No Hit
NNNNNNNNNNNNNNNNCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN169140.3278068974338848No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN163940.31772888001248123No Hit
NNNNNNNNNNNNNNNNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN163450.3167792206785413No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNANNNNNNNNNNNNNNNANNNNNNNN97290.18855582979391425No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNANNNNNNNNNNNNNNNTNNNNNNNN89520.17349694607000926No Hit
NNNNANNNNNANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN89270.17301242600167255No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNTNNNNNNNNNNNNNNNANNNNNNNN88530.1715782465993959No Hit
NNNNANNNNNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN87760.17008592478891882No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNTNNNNNNNNNNNNNNNTNNNNNNNN84990.16471744243174807No Hit
NNNNTNNNNNANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN79500.1540773817310739No Hit
NNNNTNNNNNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN74800.14496840444634376No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNANNNNNNNNNNNNNNNGNNNNNNNN67970.13173131617938483No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNGNNNNNNNNNNNNNNNANNNNNNNN67180.13020023276344084No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNTNNNNNNNNNNNNNNNGNNNNNNNN64340.1246960847871358No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNGNNNNNNNNNNNNNNNTNNNNNNNN64140.12430846873246644No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNANNNNNNNNNNNNNNNCNNNNNNNN63550.12316500137119181No Hit
NNNNGNNNNNANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN63390.1228549085274563No Hit
NNNNANNNNNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN61170.11855237032062632No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNCNNNNNNNNNNNNNNNANNNNNNNN59040.11442425933839753No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNTNNNNNNNNNNNNNNNCNNNNNNNN58710.11378469284819308No Hit
NNNNGNNNNNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN58120.11264122548691843No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNCNNNNNNNNNNNNNNNTNNNNNNNN58010.11242803665685028No Hit
NNNNANNNNNCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN55580.10771850159261746No Hit
NNNNCNNNNNANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN53780.10422995710059316No Hit
NNNNCNNNNNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN52250.10126469428237247No Hit
NNNNTNNNNNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN51610.10002432290743049No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)