FastQCFastQC Report
Mon 12 Sep 2022
EGAF00000656511

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000656511
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3901573
Sequences flagged as poor quality0
Sequence length93
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT118850.3046207260507493No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90230.2312656971944393No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77940.19976558172819014No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT51860.13292074760615782RNA PCR Primer, Index 33 (95% over 22bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40330.10336856442260596No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)