FastQCFastQC Report
Tue 13 Sep 2022
EGAF00000656559

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000656559
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5383343
Sequences flagged as poor quality0
Sequence length93
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140570.2611202741493529No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT139470.2590769341652575No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108110.20082316880050186No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT62730.11652610654754861TruSeq Adapter, Index 3 (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56860.10562210135969415No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)