FastQCFastQC Report
Thu 9 Feb 2023
EGAF00000656577

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000656577
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91626945
Sequences flagged as poor quality0
Sequence length93
%GC43

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3783780.41295494463992005No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3777880.412311029250184No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT3036580.3314068803669052No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2182890.23823668900016257No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT1562930.17057536950511665RNA PCR Primer, Index 26 (100% over 22bp)
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG1140380.12445902239783285RNA PCR Primer, Index 26 (100% over 29bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT970560.1059251729935992Clontech SMART CDS Primer II A (100% over 21bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)