FastQCFastQC Report
Tue 13 Sep 2022
EGAF00000656889

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000656889
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4658061
Sequences flagged as poor quality0
Sequence length93
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT224370.4816811115182906No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178760.3837648326202684No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161480.3466678517091124No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT101720.21837412605803147RNA PCR Primer, Index 39 (95% over 23bp)
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG79120.16985608389413534RNA PCR Primer, Index 39 (96% over 30bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75680.16247103676830338No Hit
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT67060.14396548263322442RNA PCR Primer, Index 39 (96% over 25bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA46890.10066420340995963No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)