FastQCFastQC Report
Tue 13 Sep 2022
EGAF00000656893

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000656893
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7717031
Sequences flagged as poor quality0
Sequence length93
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT218200.282751229067241No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182910.23702120673093058No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157430.20400332718632336No Hit
ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT94920.12300067214969072RNA PCR Primer, Index 31 (95% over 22bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78650.10191743430860911No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)