FastQCFastQC Report
Mon 20 May 2024
EGAF00000684032

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000684032
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences226892760
Total Bases17 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC28

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG2550700.11241874795828655Illumina Paired End PCR Primer 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)