FastQCFastQC Report
Thu 9 Feb 2023
EGAF00000684040

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000684040
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences182912432
Sequences flagged as poor quality0
Sequence length75
%GC32

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTA28858431.577718347761075Illumina Paired End PCR Primer 2 (100% over 45bp)
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCT21610541.1814691742767927Illumina Paired End PCR Primer 2 (100% over 56bp)
TCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATG8895280.48631358200955965Illumina Paired End PCR Primer 2 (100% over 47bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGC5206260.2846312819240192Illumina Paired End PCR Primer 2 (100% over 48bp)
GCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTC4859850.2656927113625606Illumina Paired End PCR Primer 2 (100% over 55bp)
GTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGC4852480.26528978631698474Illumina Paired End PCR Primer 2 (100% over 58bp)
AGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTT3221070.1760990198851Illumina Paired End PCR Primer 2 (100% over 54bp)
TCGGAAGAGCGGTTCAGCAGGAATGCCGAAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTAT2908460.15900832809439655Illumina Paired End PCR Primer 2 (100% over 46bp)
GAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCT2235390.1222109386200715Illumina Paired End PCR Primer 2 (100% over 53bp)
GATCGGAAGAGCGGTTCAGCAGGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTC2180020.11918380703614503Illumina Paired End PCR Primer 2 (100% over 52bp)
GGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTG1855550.10144471754659082Illumina Paired End PCR Primer 2 (100% over 57bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)