FastQCFastQC Report
Thu 23 Feb 2023
EGAF00000684041

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000684041
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences182912432
Sequences flagged as poor quality0
Sequence length75
%GC32

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCATTCCTGCTGAACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCA15635830.8548259858028676Illumina Single End PCR Primer 1 (100% over 56bp)
CGGCATTCCTGCTGAACCGCTCTTCCGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC5740070.3138151921789548Illumina Single End PCR Primer 1 (100% over 47bp)
CGGCATTCCTGCTGAACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATT3608840.197298781747104Illumina Single End PCR Primer 1 (100% over 58bp)
CGGCATTCCTGCTGAACCGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATC3491030.19085799482453988Illumina Single End PCR Primer 1 (100% over 55bp)
CGGCATTCCTGCTGAACCGCTCTTCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG3008480.1644765184686845Illumina Single End PCR Primer 1 (100% over 48bp)
CGGCATTCCTGCTGAACCGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTAT2363270.12920226220599376Illumina Single End PCR Primer 1 (100% over 54bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)