FastQCFastQC Report
Mon 6 Mar 2023
EGAF00000684049

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000684049
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences163891949
Sequences flagged as poor quality0
Sequence length75
%GC29

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCATTCCTGCTGAACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCA6780930.41374393564628364Illumina Single End PCR Primer 1 (100% over 56bp)
CGGCATTCCTGCTGAACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATT2022570.12340874657607495Illumina Single End PCR Primer 1 (100% over 58bp)
CGGCATTCCTGCTGAACCGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATC1654400.10094455585490658Illumina Single End PCR Primer 1 (100% over 55bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)