FastQCFastQC Report
Fri 10 Feb 2023
EGAF00000684092

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000684092
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences173828665
Sequences flagged as poor quality0
Sequence length75
%GC28

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA10918240.6281035409205955Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAC4151960.23885358608719687Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACA4141180.23823343520471724Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACAA2430400.13981583532267247Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACAAA1864130.10723950506091731Illumina Paired End PCR Primer 2 (100% over 60bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)