FastQCFastQC Report
Fri 10 Feb 2023
EGAF00000684096

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000684096
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences176286521
Sequences flagged as poor quality0
Sequence length75
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACA51019742.894137323181958Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA38061432.1590663758121362Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAC33350221.8918190574536327Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACAA29343601.6645401947662237Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACAC26243191.4886668504848422Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACACA13144950.7456582571052043Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACC11524030.6537102175837936Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACCA10573920.5998144350469087Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACAAC10190090.5780413580230561Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACAACA8520700.4833438173075071Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAGA7852370.44543224039233265Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACAAA7623220.4324335154359306Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAG6418340.3640856920649084Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAATA6103840.34624541714110973Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTA5442760.3087451025254506Illumina Paired End PCR Primer 2 (100% over 45bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACAAAC5271790.2990466866153652Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACAG5067670.287467809294393Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACG5001300.2837029156642101Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACACAC4824930.2736981802482789Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAT4689110.2659936774179122Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACAAAA4671540.2649970045072249Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACACAA4348750.24668647241611855Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACACC4101970.23268767099896426Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAGAA4093100.23218451284769526Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACAT3979950.22576598468353687Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAGC3936910.2233245047702768Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACT3784280.21466644066337892Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACGA3533400.20043506332511943Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAGAC3426840.1943903584097618Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAATC3352460.1901710908459076Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAGCA3265790.18525466277708208Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAATAA3141550.17820704510925145Illumina Paired End PCR Primer 2 (100% over 60bp)
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCT3081650.1748091676277394Illumina Paired End PCR Primer 2 (100% over 56bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAATAC2969720.16845984498156838Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAATCA2929930.16620272403015998Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACTA2846550.16147292395656274Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACAACC2320530.13163400053711424Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGACA2260080.12820492384667345Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACACCA2189250.12418703299499569Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACCAC2096320.11891550120272666Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACAGA2034750.11542289157774008Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAATACA2032440.11529185490023937Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACCC1977700.11218668272431334Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGAAC1774930.10068438527980253Illumina Paired End PCR Primer 2 (100% over 60bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)