FastQCFastQC Report
Mon 6 Feb 2023
EGAF00000684098

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000684098
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences194152433
Sequences flagged as poor quality0
Sequence length75
%GC31

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA18442490.9498974447567186Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACA10312590.5311594524288037Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAC9332590.48068364922318535Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACAA8822760.454424385194287Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACAC5624620.289701236965699Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAG4640230.23899932276408814Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACAAA4628540.23839721854013543Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTA4483180.23091031777077964Illumina Paired End PCR Primer 2 (100% over 45bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAGA4357230.22442314694042492Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAT3362640.1731958723381025Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAATA3297480.1698397464841453Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACACA3257480.16777950961860982Illumina Paired End PCR Primer 2 (100% over 60bp)
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCT3146450.16206080713909984Illumina Paired End PCR Primer 2 (100% over 56bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACAAAA3142300.1618470575643005Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAGAA3071930.1582225858586073Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACAAC2901210.149429494916502Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACAG2881800.14842976497750096Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAATAA2597300.1337763302713801Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACAACA2434920.125412798715739Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACACAA2338510.1204471128105822Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACAAAC2075670.10690929636714878Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACAT1978210.10188952924427169Illumina Paired End PCR Primer 2 (100% over 60bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)