FastQCFastQC Report
Thu 23 Feb 2023
EGAF00000684100

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000684100
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences167237243
Sequences flagged as poor quality0
Sequence length75
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA84277655.039406802466841Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACA81053714.84663036450559Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAC79757854.769144035697838Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACAA67952874.06326179390556Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACAC64517613.857849414558933Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACAAC35920502.147876833870073Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACACAA31338361.8738864285152084Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACACAC30706551.8361071642397264Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACACA30132481.8017804802008128Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACAACA30025771.7953997244501334Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACAAA28873521.726500597716742Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACAAAC28410821.6988333154954005Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACAAAA27381451.6372818344057487Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACC17439051.0427731100542001Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACCA15353550.9180700258255274Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACACC8414720.5031606506452633Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACACCA8090650.48378278993752605Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACCAC7994800.47805141107235305Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACAACC7041830.4210682903927088Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACCAA6014860.35966031800703624Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTA5426470.324477365367713Illumina Paired End PCR Primer 2 (100% over 45bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACCAAC3524460.21074611951119046Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACAG3107770.18583001873571905Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAT2932080.1753245836515016Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACAT2889610.17278507754400135Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAG2821820.1687315546095196Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACCACA2604880.15575956367565805Illumina Paired End PCR Primer 2 (100% over 60bp)
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCT2539180.15183101290422493Illumina Paired End PCR Primer 2 (100% over 56bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACCAAA2348690.14044060747880185Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACCC2176210.13012711528615667Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACACAT1747140.10447074877932544Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAACAAAAC1719720.10283116183636201Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAACAAAACA1715240.10256327892226734Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAACAAAAAA1699820.10164123549920039Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAACAACAC1698170.10154257326521461Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACACAG1693910.10128784531565137Illumina Paired End PCR Primer 2 (100% over 60bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAACAAAAAC1676900.10027072737619815Illumina Paired End PCR Primer 2 (100% over 60bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)