@HD VN:1.4 SO:coordinate @RG ID:1#151 PL:ILLUMINA PU:140828_MS8_13830_A_MS2686081-500V2_1#151 LB:11485375 DS:EGAS00001000814: The aim of this study is to ascertain whether leukaemic mutations exist within the blood of people with otherwise normal haematopoeisis. To satisfy this aim we plan to look for 7 known leukaemic mutations in the whole blood DNA of a large cohort of blood donors who have normal haematopoesis. Genomic regions around mutational sites have been amplified using a 2 step PCR process which involves barcoding of individual patients DT:2014-08-28T00:00:00+0100 SM:EGAN00001223777 PG:BamIndexDecoder CN:SC @PG ID:SCS PN:MiSeq Control Software DS:Controlling software on instrument VN:2.4.1.3 @PG ID:basecalling PN:RTA PP:SCS DS:Basecalling Package VN:1.18.54.0 @PG ID:Illumina2bam PN:Illumina2bam PP:basecalling DS:Convert Illumina BCL to BAM or SAM file VN:V1.16 CL:uk.ac.sanger.npg.illumina.Illumina2bam INTENSITY_DIR=/nfs/sf49/ILorHSorMS_sf49/analysis/140828_MS8_13830_A_MS2686081-500V2/Data/Intensities BASECALLS_DIR=/nfs/sf49/ILorHSorMS_sf49/analysis/140828_MS8_13830_A_MS2686081-500V2/Data/Intensities/BaseCalls LANE=1 OUTPUT=/dev/stdout SAMPLE_ALIAS=EGAN00001223627,EGAN00001223628,EGAN00001223629,EGAN00001223630,EGAN00001223631,EGAN00001223632,EGAN00001223633,EGAN00001223634,EGAN00001223635,EGAN00001223636,EGAN00001223637,EGAN00001223638,EGAN00001223639,EGAN00001223640,EGAN00001223641,EGAN00001223642,EGAN00001223643,EGAN00001223644,EGAN00001223645,EGAN00001223646,EGAN00001223647,EGAN00001223648,EGAN00001223649,EGAN00001223650,EGAN00001223651,EGAN00001223652,EGAN00001223653,EGAN00001223654,EGAN00001223655,EGAN00001223656,EGAN00001223657,EGAN00001223658,EGAN00001223659,EGAN00001223660,EGAN00001223661,EGAN00001223662,EGAN00001223663,EGAN00001223664,EGAN00001223665,EGAN00001223666,EGAN00001223667,EGAN00001223668,EGAN00001223669,EGAN00001223670,EGAN00001223671,EGAN00001223672,EGAN00001223673,EGAN00001223674,EGAN00001223675,EGAN00001223676,EGAN00001223677,EGAN00001223678,EGAN00001223679,EGAN00001223680,EGAN00001223681,EGAN00001223682,EGAN00001223683,EGAN00001223684,EGAN00001223685,EGAN00001223686,EGAN00001223687,EGAN00001223688,EGAN00001223689,EGAN00001223690,EGAN00001223691,EGAN00001223692,EGAN00001223693,EGAN00001223694,EGAN00001223695,EGAN00001223696,EGAN00001223697,EGAN00001223698,EGAN00001223699,EGAN00001223700,EGAN00001223701,EGAN00001223702,EGAN00001223703,EGAN00001223704,EGAN00001223705,EGAN00001223706,EGAN00001223707,EGAN00001223708,EGAN00001223709,EGAN00001223710,EGAN00001223711,EGAN00001223712,EGAN00001223713,EGAN00001223714,EGAN00001223715,EGAN00001223716,EGAN00001223717,EGAN00001223718,EGAN00001223719,EGAN00001223720,EGAN00001223721,EGAN00001223722,EGAN00001223723,EGAN00001223724,EGAN00001223725,EGAN00001223726,EGAN00001223727,EGAN00001223728,EGAN00001223729,EGAN00001223730,EGAN00001223731,EGAN00001223732,EGAN00001223733,EGAN00001223734,EGAN00001223735,EGAN00001223736,EGAN00001223737,EGAN00001223738,EGAN00001223739,EGAN00001223740,EGAN00001223741,EGAN00001223742,EGAN00001223743,EGAN00001223744,EGAN00001223745,EGAN00001223746,EGAN00001223747,EGAN00001223748,EGAN00001223749,EGAN00001223750,EGAN00001223751,EGAN00001223752,EGAN00001223753,EGAN00001223754,EGAN00001223755,EGAN00001223756,EGAN00001223757,EGAN00001223758,EGAN00001223759,EGAN00001223760,EGAN00001223761,EGAN00001223762,EGAN00001223763,EGAN00001223764,EGAN00001223765,EGAN00001223766,EGAN00001223767,EGAN00001223768,EGAN00001223769,EGAN00001223770,EGAN00001223771,EGAN00001223772,EGAN00001223773,EGAN00001223774,EGAN00001223775,EGAN00001223776,EGAN00001223777,EGAN00001223778,EGAN00001223779,EGAN00001223780,EGAN00001223781,EGAN00001223782,EGAN00001223783,EGAN00001223784,EGAN00001223785,EGAN00001223786,EGAN00001223787,EGAN00001223788,EGAN00001223789,EGAN00001223790,EGAN00001223791,EGAN00001223792,EGAN00001223793,EGAN00001223794,EGAN00001223795,EGAN00001223796,EGAN00001223797,EGAN00001223798,EGAN00001223799,EGAN00001223800,EGAN00001223801,EGAN00001223802,EGAN00001223803,EGAN00001223804,EGAN00001223805,EGAN00001223806,EGAN00001223807,EGAN00001223808,EGAN00001223809,EGAN00001223810,EGAN00001223811,EGAN00001223812,EGAN00001223813,EGAN00001223814,EGAN00001223815,EGAN00001223816,EGAN00001223817,EGAN00001223818,EGAN00001223819,EGAN00001223820,EGAN00001223821,EGAN00001223822,EGAN00001223823,EGAN00001223824,EGAN00001223825,EGAN00001223826,EGAN00001223827,EGAN00001223828,EGAN00001223829,EGAN00001223830,EGAN00001223831,EGAN00001223832,EGAN00001223833,EGAN00001223834,EGAN00001223835,EGAN00001223836,EGAN00001223837,EGAN00001223838,EGAN00001223839,EGAN00001223840,EGAN00001223841,EGAN00001223842,EGAN00001223843,EGAN00001223844,EGAN00001223845,EGAN00001223846,EGAN00001223847,EGAN00001223848,EGAN00001223849,EGAN00001223850,EGAN00001223851,EGAN00001223852,EGAN00001223853,EGAN00001223854,EGAN00001223855,EGAN00001223856,EGAN00001223857,EGAN00001223858,EGAN00001223859,EGAN00001223860,EGAN00001223861,EGAN00001223862,EGAN00001223863,EGAN00001223864,EGAN00001223865,EGAN00001223866,EGAN00001223867,EGAN00001223868,EGAN00001223869,EGAN00001223870,EGAN00001223871,EGAN00001223872,EGAN00001223873,EGAN00001223874,EGAN00001223875,EGAN00001223876,EGAN00001223877,EGAN00001223878,EGAN00001223879,EGAN00001223880,EGAN00001223881,EGAN00001223882,EGAN00001223883,EGAN00001223884,EGAN00001223885,EGAN00001223886,EGAN00001223887,EGAN00001223888,EGAN00001223889,EGAN00001223890,EGAN00001223891,EGAN00001223892,EGAN00001223893,EGAN00001223894,EGAN00001223895,EGAN00001223896,EGAN00001223897,EGAN00001223898,EGAN00001223899,EGAN00001223900,EGAN00001223901,EGAN00001223902,EGAN00001223903,EGAN00001223904,EGAN00001223905,EGAN00001223906,EGAN00001223907,EGAN00001223908,EGAN00001223909,EGAN00001223910,EGAN00001223911,EGAN00001223912,EGAN00001223913,EGAN00001223914,EGAN00001223915,EGAN00001223916,EGAN00001223917,EGAN00001223918,EGAN00001223919,EGAN00001223920,EGAN00001223921,EGAN00001223922,EGAN00001223923,EGAN00001223924,EGAN00001223925,EGAN00001223926,EGAN00001223927,EGAN00001223928,EGAN00001223929,EGAN00001223930,EGAN00001223931,EGAN00001223932,EGAN00001223933,EGAN00001223934,EGAN00001223935,EGAN00001223936,EGAN00001223937,EGAN00001223938,EGAN00001223939,EGAN00001223940,EGAN00001223941,EGAN00001223942,EGAN00001223943,EGAN00001223944,EGAN00001223945,EGAN00001223946,EGAN00001223947,EGAN00001223948,EGAN00001223949,EGAN00001223950,EGAN00001223951,EGAN00001223952,EGAN00001223953,EGAN00001223954,EGAN00001223955,EGAN00001223956,EGAN00001223957,EGAN00001223958,EGAN00001223959,EGAN00001223960,EGAN00001223961,EGAN00001223962,EGAN00001223963,EGAN00001223964,EGAN00001223965,EGAN00001223966,EGAN00001223967,EGAN00001223968,EGAN00001223969,EGAN00001223970,EGAN00001223971,EGAN00001223972,EGAN00001223973,EGAN00001223974,EGAN00001223975,EGAN00001223976,EGAN00001223977,EGAN00001223978,EGAN00001223979,EGAN00001223980,EGAN00001223981,EGAN00001223982,EGAN00001223983,EGAN00001223984,EGAN00001223985,EGAN00001223986,EGAN00001223987,EGAN00001223988,EGAN00001223989,EGAN00001223990,EGAN00001223991,EGAN00001223992,EGAN00001223993,EGAN00001223994,EGAN00001223995,EGAN00001223996,EGAN00001223997,EGAN00001223998,EGAN00001223999,EGAN00001224000,EGAN00001224001,EGAN00001224002,EGAN00001224003,EGAN00001224004,EGAN00001224005,EGAN00001224006,EGAN00001224007,EGAN00001224008,EGAN00001224009,EGAN00001224010 STUDY_NAME=EGAS00001000814: The aim of this study is to ascertain whether leukaemic mutations exist within the blood of people with otherwise normal haematopoeisis. To satisfy this aim we plan to look for 7 known leukaemic mutations in the whole blood DNA of a large cohort of blood donors who have normal haematopoesis. Genomic regions around mutational sites have been amplified using a 2 step PCR process which involves barcoding of individual patients PLATFORM_UNIT=140828_MS8_13830_A_MS2686081-500V2_1 COMPRESSION_LEVEL=0 GENERATE_SECONDARY_BASE_CALLS=false PF_FILTER=true READ_GROUP_ID=1 LIBRARY_NAME=unknown SEQUENCING_CENTER=SC PLATFORM=ILLUMINA BARCODE_SEQUENCE_TAG_NAME=BC BARCODE_QUALITY_TAG_NAME=QT VERBOSITY=INFO QUIET=false VALIDATION_STRINGENCY=STRICT MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false @PG ID:bamadapterfind PN:bamadapterfind PP:Illumina2bam VN:0.0.163 CL:bamadapterfind level=0 @PG ID:BamIndexDecoder PN:BamIndexDecoder PP:bamadapterfind DS:A command-line tool to decode multiplexed bam file VN:V1.16 CL:uk.ac.sanger.npg.picard.BamIndexDecoder INPUT=/dev/stdin OUTPUT=/nfs/sf49/ILorHSorMS_sf49/analysis/140828_MS8_13830_A_MS2686081-500V2/Data/Intensities/BAM_basecalls_20140830-062625/13830_1.bam BARCODE_FILE=/nfs/sf49/ILorHSorMS_sf49/analysis/140828_MS8_13830_A_MS2686081-500V2/lane_1.taglist METRICS_FILE=/nfs/sf49/ILorHSorMS_sf49/analysis/140828_MS8_13830_A_MS2686081-500V2/Data/Intensities/BAM_basecalls_20140830-062625/13830_1.bam.tag_decode.metrics VALIDATION_STRINGENCY=SILENT CREATE_MD5_FILE=true BARCODE_TAG_NAME=BC BARCODE_QUALITY_TAG_NAME=QT MAX_MISMATCHES=1 MIN_MISMATCH_DELTA=1 MAX_NO_CALLS=2 CONVERT_LOW_QUALITY_TO_NO_CALL=false MAX_LOW_QUALITY_TO_CONVERT=15 VERBOSITY=INFO QUIET=false COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false @PG ID:SplitBamByReadGroup PN:SplitBamByReadGroup PP:BamIndexDecoder DS:Split a BAM file into multiple BAM files based on ReadGroup. Headers are a copy of the original file, removing @RGs where IDs match with the other ReadGroup IDs VN:V1.16 CL:uk.ac.sanger.npg.picard.SplitBamByReadGroup INPUT=/nfs/sf49/ILorHSorMS_sf49/analysis/140828_MS8_13830_A_MS2686081-500V2/Data/Intensities/BAM_basecalls_20140830-062625/no_cal/13830_1.bam OUTPUT_PREFIX=/nfs/sf49/ILorHSorMS_sf49/analysis/140828_MS8_13830_A_MS2686081-500V2/Data/Intensities/BAM_basecalls_20140830-062625/no_cal/lane1/13830_1 OUTPUT_COMMON_RG_HEAD_TO_TRIM=1 VALIDATION_STRINGENCY=SILENT CREATE_MD5_FILE=true VERBOSITY=INFO QUIET=false COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false @PG ID:bwa_aln PN:bwa PP:SplitBamByReadGroup VN:0.5.10-tpx CL:/software/solexa/pkg/bwa/bwa-0.5.10-mt_fixes/bwa aln -q 15 -t 16 /lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/bwa/human_g1k_v37.fasta -b1 /nfs/sf49/ILorHSorMS_sf49/analysis/140828_MS8_13830_A_MS2686081-500V2/Data/Intensities/BAM_basecalls_20140830-062625/no_cal/lane1/13830_1#151.bam > /tmp/yOTsXXUvCd/1.sai @PG ID:bwa_aln_1 PN:bwa PP:bwa_aln VN:0.5.10-tpx CL:/software/solexa/pkg/bwa/bwa-0.5.10-mt_fixes/bwa aln -q 15 -t 16 /lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/bwa/human_g1k_v37.fasta -b2 /nfs/sf49/ILorHSorMS_sf49/analysis/140828_MS8_13830_A_MS2686081-500V2/Data/Intensities/BAM_basecalls_20140830-062625/no_cal/lane1/13830_1#151.bam > /tmp/yOTsXXUvCd/2.sai @PG ID:bwa_sam PN:bwa PP:bwa_aln_1 VN:0.5.10-tpx CL:/software/solexa/pkg/bwa/bwa-0.5.10-mt_fixes/bwa sampe -t 6 /lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/bwa/human_g1k_v37.fasta /tmp/yOTsXXUvCd/1.sai /tmp/yOTsXXUvCd/2.sai /nfs/sf49/ILorHSorMS_sf49/analysis/140828_MS8_13830_A_MS2686081-500V2/Data/Intensities/BAM_basecalls_20140830-062625/no_cal/lane1/13830_1#151.bam /nfs/sf49/ILorHSorMS_sf49/analysis/140828_MS8_13830_A_MS2686081-500V2/Data/Intensities/BAM_basecalls_20140830-062625/no_cal/lane1/13830_1#151.bam @PG ID:Picard_SamFormatConverter PN:SamFormatConverter PP:bwa_sam VN:1.72(1230) CL:/software/jdk1.7.0_25/bin/java -Xmx1000m -jar /software/solexa/pkg/picard/picard-tools-1.72/SamFormatConverter.jar VALIDATION_STRINGENCY=SILENT INPUT=/dev/stdin OUTPUT=/dev/stdout COMPRESSION_LEVEL=0 @PG ID:samtools_fixmate PN:samtools PP:Picard_SamFormatConverter VN:0.1.18 (r982:295) CL:/software/solexa/pkg/samtools/samtools-0.1.18/samtools fixmate - - @PG ID:BamMerger PN:BamMerger PP:samtools_fixmate DS:A command-line tool to merge BAM/SAM alignment info in the first input file with the data in an unmapped BAM file, producing a third BAM file that has alignment data and all the additional data from the unmapped BAM VN:V1.16 CL:uk.ac.sanger.npg.picard.BamMerger ALIGNED_BAM=/dev/stdin ALIGNMENT_PROGRAM_ID=NULL KEEP_ALL_PG=true INPUT=/nfs/sf49/ILorHSorMS_sf49/analysis/140828_MS8_13830_A_MS2686081-500V2/Data/Intensities/BAM_basecalls_20140830-062625/no_cal/lane1/13830_1#151.bam OUTPUT=/nfs/sf49/ILorHSorMS_sf49/analysis/140828_MS8_13830_A_MS2686081-500V2/Data/Intensities/BAM_basecalls_20140830-062625/no_cal/archive/lane1/13830_1#151.bam KEEP_EXTRA_UNMAPPED_READS=true VALIDATION_STRINGENCY=SILENT CREATE_MD5_FILE=true REPLACE_ALIGNED_BASE_QUALITY=false VERBOSITY=INFO QUIET=false COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false @PG ID:bamsort PN:bamsort PP:BamMerger VN:0.0.163 CL:/software/hpag/biobambam/0.0.163/bin/bamsort tmpfile=/nfs/sf49/ILorHSorMS_sf49/analysis/140828_MS8_13830_A_MS2686081-500V2/Data/Intensities/BAM_basecalls_20140830-062625/no_cal/archive/lane1/E9W7tKcyML/ @PG ID:bammarkduplicates PN:bammarkduplicates PP:bamsort VN:0.0.163 CL:/software/hpag/biobambam/0.0.163/bin/bammarkduplicates I=/nfs/sf49/ILorHSorMS_sf49/analysis/140828_MS8_13830_A_MS2686081-500V2/Data/Intensities/BAM_basecalls_20140830-062625/no_cal/archive/lane1/E9W7tKcyML/sorted.bam O=/dev/stdout tmpfile=/nfs/sf49/ILorHSorMS_sf49/analysis/140828_MS8_13830_A_MS2686081-500V2/Data/Intensities/BAM_basecalls_20140830-062625/no_cal/archive/lane1/E9W7tKcyML/ M=/tmp/dQkDeLzzt0 level=0 @PG ID:BamTagStripper PN:BamTagStripper PP:bammarkduplicates DS:Strip a list of tags in bam/sam record. By default, any tags containing lowercase letters will be stripped and other tags will be kept. A list of tags can be given to keep or strip VN:V1.16 CL:uk.ac.sanger.npg.picard.BamTagStripper INPUT=/dev/stdin OUTPUT=/dev/stdout TAG_TO_KEEP=[a3, aa, af, ah, as, br, qr, tq, tr] TAG_TO_STRIP=[OQ] TMP_DIR=[/nfs/sf49/ILorHSorMS_sf49/analysis/140828_MS8_13830_A_MS2686081-500V2/Data/Intensities/BAM_basecalls_20140830-062625/no_cal/archive/lane1/E9W7tKcyML] VERBOSITY=INFO VALIDATION_STRINGENCY=SILENT CREATE_INDEX=false CREATE_MD5_FILE=false QUIET=false COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 @PG ID:scramble PN:scramble PP:BamTagStripper VN:1.13.5 CL:/software/badger/bin/scramble -I bam -O cram -r /lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta @SQ SN:1 LN:249250621 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:1b22b98cdeb4a9304cb5d48026a85128 SP:Human @SQ SN:2 LN:243199373 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:a0d9851da00400dec1098a9255ac712e SP:Human @SQ SN:3 LN:198022430 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:fdfd811849cc2fadebc929bb925902e5 SP:Human @SQ SN:4 LN:191154276 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:23dccd106897542ad87d2765d28a19a1 SP:Human @SQ SN:5 LN:180915260 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:0740173db9ffd264d728f32784845cd7 SP:Human @SQ SN:6 LN:171115067 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:1d3a93a248d92a729ee764823acbbc6b SP:Human @SQ SN:7 LN:159138663 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:618366e953d6aaad97dbe4777c29375e SP:Human @SQ SN:8 LN:146364022 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:96f514a9929e410c6651697bded59aec SP:Human @SQ SN:9 LN:141213431 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:3e273117f15e0a400f01055d9f393768 SP:Human @SQ SN:10 LN:135534747 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:988c28e000e84c26d552359af1ea2e1d SP:Human @SQ SN:11 LN:135006516 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:98c59049a2df285c76ffb1c6db8f8b96 SP:Human @SQ SN:12 LN:133851895 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:51851ac0e1a115847ad36449b0015864 SP:Human @SQ SN:13 LN:115169878 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:283f8d7892baa81b510a015719ca7b0b SP:Human @SQ SN:14 LN:107349540 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:98f3cae32b2a2e9524bc19813927542e SP:Human @SQ SN:15 LN:102531392 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:e5645a794a8238215b2cd77acb95a078 SP:Human @SQ SN:16 LN:90354753 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:fc9b1a7b42b97a864f56b348b06095e6 SP:Human @SQ SN:17 LN:81195210 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:351f64d4f4f9ddd45b35336ad97aa6de SP:Human @SQ SN:18 LN:78077248 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:b15d4b2d29dde9d3e4f93d1d0f2cbc9c SP:Human @SQ SN:19 LN:59128983 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:1aacd71f30db8e561810913e0b72636d SP:Human @SQ SN:20 LN:63025520 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:0dec9660ec1efaaf33281c0d5ea2560f SP:Human @SQ SN:21 LN:48129895 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:2979a6085bfe28e3ad6f552f361ed74d SP:Human @SQ SN:22 LN:51304566 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:a718acaa6135fdca8357d5bfe94211dd SP:Human @SQ SN:X LN:155270560 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:7e0e2e580297b7764e31dbc80c2540dd SP:Human @SQ SN:Y LN:59373566 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:1fa3474750af0948bdf97d5a0ee52e51 SP:Human @SQ SN:MT LN:16569 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:c68f52674c9fb33aef52dcf399755519 SP:Human @SQ SN:GL000207.1 LN:4262 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:f3814841f1939d3ca19072d9e89f3fd7 SP:Human @SQ SN:GL000226.1 LN:15008 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:1c1b2cd1fccbc0a99b6a447fa24d1504 SP:Human @SQ SN:GL000229.1 LN:19913 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:d0f40ec87de311d8e715b52e4c7062e1 SP:Human @SQ SN:GL000231.1 LN:27386 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:ba8882ce3a1efa2080e5d29b956568a4 SP:Human @SQ SN:GL000210.1 LN:27682 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:851106a74238044126131ce2a8e5847c SP:Human @SQ SN:GL000239.1 LN:33824 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:99795f15702caec4fa1c4e15f8a29c07 SP:Human @SQ SN:GL000235.1 LN:34474 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:118a25ca210cfbcdfb6c2ebb249f9680 SP:Human @SQ SN:GL000201.1 LN:36148 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:dfb7e7ec60ffdcb85cb359ea28454ee9 SP:Human @SQ SN:GL000247.1 LN:36422 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:7de00226bb7df1c57276ca6baabafd15 SP:Human @SQ SN:GL000245.1 LN:36651 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:89bc61960f37d94abf0df2d481ada0ec SP:Human @SQ SN:GL000197.1 LN:37175 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:6f5efdd36643a9b8c8ccad6f2f1edc7b SP:Human @SQ SN:GL000203.1 LN:37498 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:96358c325fe0e70bee73436e8bb14dbd SP:Human @SQ SN:GL000246.1 LN:38154 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:e4afcd31912af9d9c2546acf1cb23af2 SP:Human @SQ SN:GL000249.1 LN:38502 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:1d78abec37c15fe29a275eb08d5af236 SP:Human @SQ SN:GL000196.1 LN:38914 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:d92206d1bb4c3b4019c43c0875c06dc0 SP:Human @SQ SN:GL000248.1 LN:39786 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:5a8e43bec9be36c7b49c84d585107776 SP:Human @SQ SN:GL000244.1 LN:39929 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:0996b4475f353ca98bacb756ac479140 SP:Human @SQ SN:GL000238.1 LN:39939 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:131b1efc3270cc838686b54e7c34b17b SP:Human @SQ SN:GL000202.1 LN:40103 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:06cbf126247d89664a4faebad130fe9c SP:Human @SQ SN:GL000234.1 LN:40531 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:93f998536b61a56fd0ff47322a911d4b SP:Human @SQ SN:GL000232.1 LN:40652 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:3e06b6741061ad93a8587531307057d8 SP:Human @SQ SN:GL000206.1 LN:41001 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:43f69e423533e948bfae5ce1d45bd3f1 SP:Human @SQ SN:GL000240.1 LN:41933 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:445a86173da9f237d7bcf41c6cb8cc62 SP:Human @SQ SN:GL000236.1 LN:41934 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:fdcd739913efa1fdc64b6c0cd7016779 SP:Human @SQ SN:GL000241.1 LN:42152 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:ef4258cdc5a45c206cea8fc3e1d858cf SP:Human @SQ SN:GL000243.1 LN:43341 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:cc34279a7e353136741c9fce79bc4396 SP:Human @SQ SN:GL000242.1 LN:43523 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:2f8694fc47576bc81b5fe9e7de0ba49e SP:Human @SQ SN:GL000230.1 LN:43691 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:b4eb71ee878d3706246b7c1dbef69299 SP:Human @SQ SN:GL000237.1 LN:45867 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:e0c82e7751df73f4f6d0ed30cdc853c0 SP:Human @SQ SN:GL000233.1 LN:45941 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:7fed60298a8d62ff808b74b6ce820001 SP:Human @SQ SN:GL000204.1 LN:81310 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:efc49c871536fa8d79cb0a06fa739722 SP:Human @SQ SN:GL000198.1 LN:90085 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:868e7784040da90d900d2d1b667a1383 SP:Human @SQ SN:GL000208.1 LN:92689 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:aa81be49bf3fe63a79bdc6a6f279abf6 SP:Human @SQ SN:GL000191.1 LN:106433 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:d75b436f50a8214ee9c2a51d30b2c2cc SP:Human @SQ SN:GL000227.1 LN:128374 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:a4aead23f8053f2655e468bcc6ecdceb SP:Human @SQ SN:GL000228.1 LN:129120 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:c5a17c97e2c1a0b6a9cc5a6b064b714f SP:Human @SQ SN:GL000214.1 LN:137718 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:46c2032c37f2ed899eb41c0473319a69 SP:Human @SQ SN:GL000221.1 LN:155397 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:3238fb74ea87ae857f9c7508d315babb SP:Human @SQ SN:GL000209.1 LN:159169 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:f40598e2a5a6b26e84a3775e0d1e2c81 SP:Human @SQ SN:GL000218.1 LN:161147 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:1d708b54644c26c7e01c2dad5426d38c SP:Human @SQ SN:GL000220.1 LN:161802 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:fc35de963c57bf7648429e6454f1c9db SP:Human @SQ SN:GL000213.1 LN:164239 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:9d424fdcc98866650b58f004080a992a SP:Human @SQ SN:GL000211.1 LN:166566 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:7daaa45c66b288847b9b32b964e623d3 SP:Human @SQ SN:GL000199.1 LN:169874 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:569af3b73522fab4b40995ae4944e78e SP:Human @SQ SN:GL000217.1 LN:172149 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:6d243e18dea1945fb7f2517615b8f52e SP:Human @SQ SN:GL000216.1 LN:172294 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:642a232d91c486ac339263820aef7fe0 SP:Human @SQ SN:GL000215.1 LN:172545 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:5eb3b418480ae67a997957c909375a73 SP:Human @SQ SN:GL000205.1 LN:174588 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:d22441398d99caf673e9afb9a1908ec5 SP:Human @SQ SN:GL000219.1 LN:179198 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:f977edd13bac459cb2ed4a5457dba1b3 SP:Human @SQ SN:GL000224.1 LN:179693 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:d5b2fc04f6b41b212a4198a07f450e20 SP:Human @SQ SN:GL000223.1 LN:180455 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:399dfa03bf32022ab52a846f7ca35b30 SP:Human @SQ SN:GL000195.1 LN:182896 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:5d9ec007868d517e73543b005ba48535 SP:Human @SQ SN:GL000212.1 LN:186858 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:563531689f3dbd691331fd6c5730a88b SP:Human @SQ SN:GL000222.1 LN:186861 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:6fe9abac455169f50470f5a6b01d0f59 SP:Human @SQ SN:GL000200.1 LN:187035 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:75e4c8d17cd4addf3917d1703cacaf25 SP:Human @SQ SN:GL000193.1 LN:189789 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:dbb6e8ece0b5de29da56601613007c2a SP:Human @SQ SN:GL000194.1 LN:191469 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:6ac8f815bf8e845bb3031b73f812c012 SP:Human @SQ SN:GL000225.1 LN:211173 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:63945c3e6962f28ffd469719a747e73c SP:Human @SQ SN:GL000192.1 LN:547496 UR:/lustre/scratch110/srpipe/references/Homo_sapiens/1000Genomes/all/fasta/human_g1k_v37.fasta AS:NCBI37 M5:325ba9e808f669dfeee210fdd7b470ac SP:Human