FastQCFastQC Report
Sun 14 May 2023
EGAF00000692057

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000692057
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences60009744
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG8878401.479493063659795Illumina Paired End PCR Primer 2 (100% over 31bp)
CGTTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG2259470.37651718694217395Illumina Paired End PCR Primer 2 (100% over 31bp)
CTGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG1444500.24071090854845173Illumina Paired End PCR Primer 2 (100% over 31bp)
CTTTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG901080.15015561472816816Illumina Paired End PCR Primer 2 (100% over 31bp)
AGTTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG682140.11367153974194591Illumina Paired End PCR Primer 2 (100% over 31bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)