FastQCFastQC Report
Sun 5 Mar 2023
EGAF00000692070

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000692070
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences161164651
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCATTCCTGCTGAACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT41282872.561533794405077Illumina Single End PCR Primer 1 (100% over 31bp)
CGGCATTCCTGCTGAACCGCTCTTCCGAGATCGGAAGAGCGTCGTGTAGG2495420.1548366831384135Illumina Paired End Adapter 2 (96% over 28bp)
CGGCATTCCTGCTGAACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGG2481700.15398537983369567Illumina Single End PCR Primer 1 (96% over 30bp)
CGGCATTCCTGCTGAACCGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAG1742540.10812172453375027Illumina Single End PCR Primer 1 (100% over 30bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)