FastQCFastQC Report
Sun 2 Apr 2023
EGAF00000692085

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000692085
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences38969114
Sequences flagged as poor quality0
Sequence length50
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG23617476.060561192127694Illumina Paired End PCR Primer 2 (100% over 31bp)
NGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG13976803.586635303024852Illumina Paired End PCR Primer 2 (100% over 31bp)
CGTTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG4666921.1975945873442233Illumina Paired End PCR Primer 2 (100% over 31bp)
CTGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG2676260.6867643950026681Illumina Paired End PCR Primer 2 (100% over 31bp)
AGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG1689200.43347149232081594Illumina Paired End PCR Primer 2 (100% over 31bp)
AGTTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG1574570.4040558889791541Illumina Paired End PCR Primer 2 (100% over 31bp)
CGGTTCAGCCGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG853290.21896571731140718Illumina Paired End PCR Primer 2 (100% over 31bp)
CTTTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG619690.1590208081199896Illumina Paired End PCR Primer 2 (100% over 31bp)
CGGTTCCGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG528920.13572800243803337Illumina Paired End PCR Primer 2 (100% over 31bp)
GCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCC451100.11575834133668013Illumina Paired End PCR Primer 2 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)