FastQCFastQC Report
Tue 27 Sep 2022
EGAF00000692086

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000692086
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences38969114
Sequences flagged as poor quality0
Sequence length50
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCATTCCTGCTGAACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT431552411.07421636529894Illumina Single End PCR Primer 1 (100% over 31bp)
CGGCATTCCTGCTGAACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGG2760100.7082788692604097Illumina Single End PCR Primer 1 (96% over 30bp)
CGGCCTTCCTGCTGAACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT781730.200602456601913Illumina Single End PCR Primer 1 (100% over 31bp)
CGGCATTCCTGCTGAACCGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAG744630.1910820964520774Illumina Single End PCR Primer 1 (100% over 30bp)
CGGCATTCCTGCTGACCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT716700.18391488192418232Illumina Single End PCR Primer 1 (100% over 31bp)
CGGCATTCCTGCTGAACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGA685230.17583925567309536Illumina Single End PCR Primer 1 (96% over 30bp)
CGGCATTCCTGCTGAACCGAGATCGGAAGAGCGTCGTGTAGGGACAGAGT562030.14422447479816963Illumina Single End PCR Primer 1 (96% over 31bp)
CGGCATTCCTGCTGAACCGAGATCGGAAGAGCGTCGTGTAGGGCAAGAGT530370.13610009198566844Illumina Single End PCR Primer 1 (96% over 31bp)
CGGCATTCCTGCTGAACCGAGATCGGAAGAGCGTCGTGTAGGGGAAGAGT490020.125745738022168Illumina Single End PCR Primer 1 (96% over 31bp)
CGGCATTCCTGCTGAACCGAGATCGGAAGCGCGTCGTGTAGGGAAAGAGT469200.12040304534509047Illumina Single End PCR Primer 1 (96% over 31bp)
CGGCATTCCTGCTGAACCGAGATCGGAAGAGCGTCGTGGAGGGAAAGAGT412110.10575298170751328Illumina Single End PCR Primer 1 (96% over 31bp)
CGGCATTCATGCTGAACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT395310.10144187522456886Illumina Single End PCR Primer 1 (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)