FastQCFastQC Report
Mon 6 Feb 2023
EGAF00000692117

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000692117
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences178505899
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTA14977980.8390747915843386Illumina Paired End PCR Primer 2 (100% over 45bp)
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCT8415960.4714667720868989Illumina Paired End PCR Primer 2 (100% over 56bp)
CGTTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCT2341900.13119454388451332Illumina Paired End PCR Primer 2 (100% over 56bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)