FastQCFastQC Report
Wed 15 Feb 2023
EGAF00000692119

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000692119
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences187545944
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTA16015690.8539608833129444Illumina Paired End PCR Primer 2 (100% over 45bp)
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCT13062230.6964816045288614Illumina Paired End PCR Primer 2 (100% over 56bp)
CGTTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCT4469060.23829147699403191Illumina Paired End PCR Primer 2 (100% over 56bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)