FastQCFastQC Report
Mon 23 Jan 2023
EGAF00000692129

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000692129
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences260791400
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCT71725482.750300815134241Illumina Paired End PCR Primer 2 (100% over 56bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTA31533321.2091395651850483Illumina Paired End PCR Primer 2 (100% over 45bp)
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATATCGTATGCCGTCTTCT3446720.132163867366792Illumina Paired End PCR Primer 2 (98% over 56bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)