FastQCFastQC Report
Fri 10 Feb 2023
EGAF00000692131

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000692131
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences175230458
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCT51284742.926702388690898Illumina Paired End PCR Primer 2 (100% over 56bp)
CGTTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCT27368281.5618449162530865Illumina Paired End PCR Primer 2 (100% over 56bp)
CTGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCT16125240.9202304316296428Illumina Paired End PCR Primer 2 (100% over 56bp)
CTTTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCT13428460.7663313874349401Illumina Paired End PCR Primer 2 (100% over 56bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTA10105430.57669369328476Illumina Paired End PCR Primer 2 (100% over 45bp)
GCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTC2526350.14417299531340608Illumina Paired End PCR Primer 2 (100% over 55bp)
GCTGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTC1785120.10187270069225066Illumina Paired End PCR Primer 2 (100% over 55bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)