FastQCFastQC Report
Fri 10 Feb 2023
EGAF00000692132

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000692132
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences175230458
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCATTCCTGCTGAACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCA112603916.426046663645654Illumina Single End PCR Primer 1 (100% over 56bp)
CGGCATTCCTGCTGAACCGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATC3820980.21805455761577708Illumina Single End PCR Primer 1 (100% over 55bp)
CGGCATTCCTGCTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAA3342980.19077619485534872Illumina Single End PCR Primer 1 (100% over 58bp)
CGGCATTCCTGCTGAACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGTCGCCGTATCA2247720.12827222080307524Illumina Single End PCR Primer 1 (98% over 56bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)