FastQCFastQC Report
Tue 7 Feb 2023
EGAF00000692133

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000692133
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences248183254
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCT88724183.574946277398716Illumina Paired End PCR Primer 2 (100% over 56bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTA81416373.2804940981231554Illumina Paired End PCR Primer 2 (100% over 45bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATATCGTA3522190.14191892253939098Illumina Paired End PCR Primer 2 (97% over 45bp)
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATATCGTATGCCGTCTTCT3331810.13424797790748605Illumina Paired End PCR Primer 2 (98% over 56bp)
GCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTC2867260.11552995432963417Illumina Paired End PCR Primer 2 (100% over 55bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)