FastQCFastQC Report
Mon 6 Mar 2023
EGAF00000692136

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000692136
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences144212654
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCATTCCTGCTGAACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCA92715586.429087699890747Illumina Single End PCR Primer 1 (100% over 56bp)
CGGCATTCCTGCTGAACCGCTCTTCCGATCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG10705910.7423696675050443Illumina Single End PCR Primer 1 (100% over 45bp)
CGGCATTCCTGCTGAACCGCTCTTCCGATCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGAGTAGATCTCGGTGG5430530.3765640427087626Illumina Single End PCR Primer 1 (97% over 45bp)
CGGCATTCCTGCTGAACCGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATC3408180.2363301628163642Illumina Single End PCR Primer 1 (100% over 55bp)
CGGCATTCCTGCTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAA2131740.14781920593459158Illumina Single End PCR Primer 1 (100% over 58bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)