FastQCFastQC Report
Wed 15 Mar 2023
EGAF00000692137

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000692137
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences124987881
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCT2702086721.618789584887836Illumina Paired End PCR Primer 2 (100% over 56bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTA18617881.4895748172576828Illumina Paired End PCR Primer 2 (100% over 45bp)
GCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTC12523991.0020163474889217Illumina Paired End PCR Primer 2 (100% over 55bp)
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCGGGAATGCCGAGACCGATCTCGTATGCCGTCTTCT8777580.7022744869160555Illumina Paired End PCR Primer 2 (98% over 56bp)
CAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTG7018520.5615360420423481Illumina Paired End PCR Primer 2 (100% over 61bp)
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATATCGTATGCCGTCTTCT3150990.25210364195229457Illumina Paired End PCR Primer 2 (98% over 56bp)
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCTGGAATGCCGAGACCGATCTCGTATGCCGTCTTCT2175780.17407927733409612Illumina Paired End PCR Primer 2 (98% over 56bp)
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCT2074690.16599129318785713Illumina Paired End PCR Primer 2 (100% over 59bp)
AGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTT2017420.16140924894950415Illumina Paired End PCR Primer 2 (100% over 54bp)
GAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCT1882810.15063940479157337Illumina Paired End PCR Primer 2 (100% over 53bp)
CGGTTCAGCAGGAATGCCGAGATCGGAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTG1470250.11763140459993879Illumina Paired End PCR Primer 2 (98% over 51bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)