FastQCFastQC Report
Fri 24 Mar 2023
EGAF00000692138

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000692138
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences124987881
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCATTCCTGCTGAACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCA3198928025.593905380314432Illumina Single End PCR Primer 1 (100% over 56bp)
CGGCATTCCTGCTGAACCGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATC14940701.1953718936958375Illumina Single End PCR Primer 1 (100% over 55bp)
CGGCATTCCTGCTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAA9428950.7543891395358563Illumina Single End PCR Primer 1 (100% over 58bp)
CGGCATTCCTGCTGAACCGCTCTTCCGATCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG6527270.5222322314593044Illumina Single End PCR Primer 1 (100% over 45bp)
CGGCATTCCTGCTGAAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTA2580700.2064760182629226Illumina Single End PCR Primer 1 (100% over 58bp)
CGGCATTCCTGCTGAACCGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTAT2279810.18240248428565645Illumina Single End PCR Primer 1 (100% over 54bp)
CGGCATTCCTGCTGAACCGAGATCGGAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCAT2252480.18021587228925018Illumina Single End PCR Primer 1 (98% over 51bp)
CGGCATTCCTGCTGAACCGCTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTA2177860.17424569346847316Illumina Single End PCR Primer 1 (100% over 53bp)
CGGCATTCCTGCTGAACCGCTCTTCCGATCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGAGTAGATCTCGGTGG1695290.13563635021542608Illumina Single End PCR Primer 1 (97% over 45bp)
CGGCATTCCTGCTGAACCGAGATCGGAAAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCAT1679490.1343722276562157Illumina Single End PCR Primer 1 (100% over 47bp)
CGGCATTCCTGCTGAACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATT1489420.11916515329994273Illumina Single End PCR Primer 1 (100% over 58bp)
CGGCATTCCTGCTGAACCGAGATCGGAAGGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCAT1347800.10783445476605848Illumina Single End PCR Primer 1 (97% over 47bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)