FastQCFastQC Report
Sat 11 Mar 2023
EGAF00000692139

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000692139
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences154546269
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCT105787466.845034867842717Illumina Paired End PCR Primer 2 (100% over 56bp)
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCGGGAATGCCGAGACCGATCTCGTATGCCGTCTTCT8086200.5232219485026843Illumina Paired End PCR Primer 2 (98% over 56bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTA6855770.44360630925357375Illumina Paired End PCR Primer 2 (100% over 45bp)
GCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTC3740840.24205307732145898Illumina Paired End PCR Primer 2 (100% over 55bp)
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATATCGTATGCCGTCTTCT3470580.22456575771492743Illumina Paired End PCR Primer 2 (98% over 56bp)
CAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTG2028280.13124095541898848Illumina Paired End PCR Primer 2 (100% over 61bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)