FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000695469

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000695469
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences267910
Sequences flagged as poor quality0
Sequence length108
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGCCCCACCAGTTTCATCGAGTTCTAGCCCAACCTTCTTGTCCATTTT3280912.246276734724349No Hit
GAACAAAGGAACTGATGCCCTCTAGCAGTCTGGTTACCTTTCTTTTCAAT194127.245716845209212No Hit
GACAGGGAAAGATCTGCTGGACCCATCTGCAGTACACACATGTCCTACCT171356.395804561233249No Hit
GGAAAAAGAAGATGTCCCTGTGGAAATGTCCAATGGTGAACCAGGTTGCC148005.524243216005375No Hit
AGCCCTTACAACAAAAACAAGAGCTTACAGTAATGTCCGGGATGGGGCGG116454.346608935836661No Hit
CCTTTGCATCTCTTTCTCGTTGCCCTCACGCCGGAAGCGCTCCAGGTATT102863.8393490351237354No Hit
TGGCTGTGACCTACCTTGTAAGACACCTTCACCACGGAGGGGGCCCACTT66202.4709790601321338No Hit
ACTTCAGGAGAAGGATCAAAGAGTAAGCGCTTCATCGCCTCTGCTGTGCA61892.310104139449815No Hit
CTCAGAAGGAGCCATCACCTTTGCTGGAAAAGAAGATCCAGTTGCTAGAA60582.2612071217946323No Hit
CCAGCCCCACCAGTTTCATCGAGTTCTAGCCCAACCTTCTTGTCCATTTT44691.6680974954275691No Hit
TGCTCCTTTAGTGATCAACCCACCGAGCTGTTTACGTTTGACGTCTTCTG43751.6330110858123996No Hit
CATCTTCAGTGCCAGCACCTCGGCTTCCAGCACCTTCTGCTCCATCAGGC37081.3840468814154006No Hit
GAGTCCAGCACCTCCTTCACCTTGCCGTAAGAGCCTTCCCCCAGCAGGTC27891.0410212384756075No Hit
CAGGAAGATGAAGTACTCAGAGCCGGGCTCGTTGAAGGTTTTCAGCAGCA21870.8163189130678212No Hit
GACAGTAAGATACAGTCTATCGGGTTTAAGTTATACAACATAGTACAGTA20060.7487589115747826No Hit
CGCCCTTACAACAAAAACAAGAGCTTACAGTAATGTCCGGGATGGGGCGG19260.7188981374342129No Hit
TGGGATTTGTACCTTCGAACAAGAAATGAGTTTGTTCAGAAAGGAGAAGC18860.7039677503639281No Hit
GGTGGTGAGCAGCAGGTTCGGTGGGGTCATGTGTGTGTAGAACCATGTCG18830.7028479713336568No Hit
GAAGACGCGACGTCCATTTGTCGAAAGAACAGGAGAGCCGCCTACCCTCT16850.6289425553357471No Hit
CATCCTGCTCCTCGTGCTCCAGGATGGCCTGCAGGAAGGCGCGCCGCTCA15260.569594266731365No Hit
GGGAGGAAAGAGAACATCACCAGGACTAAGGCAGCCCCAGCTGGTGGCCC15020.5606360344891942No Hit
CAAAAATAGGCTGTCCGTCAACATTGAGAGATGGCACAAATAATTCAGTT14960.5583964764286514No Hit
CCCGGCGTCCTGAACCCCCCCGGGACTCACATTCGGATTCGGAGCCTTCC14160.5285357022880818No Hit
CAAGACGTTGAACTTCCCACTCCTCCGTGCGAATCGCTGTAGAACCATGT13760.5136053152177971No Hit
CATTGATGCGTTTGTGCTCCATGAGGTACGTGATGAGCGCGATGGTCTGG12670.47292001045127097No Hit
CAAGACGTTGAACTTCCCACTCCGGAGCTGGGGGACAAAGGCCCGTCTTG11590.432607965361502No Hit
CCTTCAGGAGAAGGATCAAAGAGTAAGCGCTTCATCGCCTCTGCTGTGCA11140.41581127990743155No Hit
CAGCCCCACCAGTTTCATCGAGTTCTAGCCCAACCTTCTTGTCCATTTTG10250.3825911686760479No Hit
CGGCTGTGACCTACCTTGTAAGACACCTTCACCACGGAGGGGGCCCACTT9940.3710201186965772No Hit
ACCGGAGGAAGAAAGAGGAGGCATGTTGGGCCCTCTGTAGAACCATGTCG9370.34974431712142134No Hit
GAACCAGCTCCTCTTCAGCATTCTCAGGATAAAATTTGGCCAAGAAGTGA8940.3336941510208652No Hit
CGCTCCTTTAGTGATCAACCCACCGAGCTGTTTACGTTTGACGTCTTCTG8100.30234033817326716No Hit
CCCACGTTCTAAGAGAGTGACAGAAAGGTAAAGAGGAGCAGCCGCAGAAA7180.2680004479116121No Hit
TTCTCCACATTACCAGTCTTCTCCAGCTTACTTGAAGGAGATCCTGGAGC7120.2657608898510694No Hit
GGCAGCACCATCACCACATAGAGAGTACCTGGGAACAACCTACTTTGAGG7030.26240155276025534No Hit
CAGGGAGGGTGGGCTCCCTGCCCCTCACAGGTGTAGAACCATGTCGTCAG6830.25493635922511293No Hit
CGTTAAACCACTGCTCGAAGGTGCTGCAGCTCTTGAAGATGGTGGGCAGC6590.24597812698294202No Hit
GGCTCTGAGAAGCCATAGTGCCCATCTCGGTGGAAGACCTTGATCAGCTT5960.2224627673472435No Hit
GGTCCCAACACCGGAAAGGATATATTTTCTGCTTCTCTTCGTTGTATAAC5710.21313127542831548No Hit
CAGGTTTTCAGCTTTGTTTCGGTTGCTGCTTCCAACATTCCATGTCTTCT5600.20902541898398713No Hit
ACGTGTGCAGAACTGAAGGCCACGCTGGAATTGTAGCCCACGTGCCACAC5500.20529282221641598No Hit
CGTCAGACCTTAGAACTGAGGGAAATTTTGCCAACTTAAAATTCTAATTT5130.19148221417640252No Hit
CTTGAAAGCCAGGAAATGCACTCTTACCTCTTCCTCTTCTTCTGAGCCAC4940.18439028031801724No Hit
GTCTGCGGCCCTTCGCCAGCCTCCTCGCCAGTCTGGTGCCCGGTGCCCCC4890.18252398193423164No Hit
CTGCAGGCCAACTGAAATACCAGAAAATGACATTCTGCTTTGTGAGAGCC4840.18065768355044604No Hit
TGGGTCCCAGTCTGCAGTTAAGGGGGCAGGAGTGGCGCTGCTCACCTCTG4530.16908663357097534No Hit
TGCCTGCTTCTTCAAGTCTTCTTCCCTTTCTGCTTCTTGAGTTGTAGAAC4190.15639580456123325No Hit
CCTCCCTGGCTTGTACTGCACCAGATCAGGGTGGGGTGGGGCAGGGCAGA4030.15042364973311934No Hit
CCTTTGCATCTCTTTCTCGTTGCCCTCACGCCGGAAGCGCTCCAGGCATT4020.15005039005636223No Hit
TGTTCAATGAGGTTCCCAGAAGAGAAAAAACAAACCTGGAGCTAAAGAAA3970.14818409167257662No Hit
GGTGGTGAGCAGCAGGTTCGGTGGGATGGGCACTGTAGAACCATGTCGTC3410.1272815497741779No Hit
TCATTTCACTGTAAACGGCTGCTGGAATGACAGGATTTGGTAAGTCCAGG3320.12392221268336381No Hit
ACACACCTTTTGTGTTTCATCCTTCTTCTCCTGTTTGAGAATGTCAGTTA3270.12205591429957821No Hit
CGACATCAGCTCGCCCTCCGTGAACATGCCCAGCTGCTGCGGGTCCACCA3250.12130939494606398No Hit
ACTTCAGGAGAAGGATCAAAGAGTAAGCGCTTCATCGCCTTTGCTGTGCA3080.11496398044119295No Hit
CTGCTGAACTTCCAAAGAATCGGCTTTCCTTCTCCTCATAAATAGATCAT3040.11347094173416446No Hit
CGCTTTGGTTCGCAAATCCCCGGCCAGGGACCCGGGAAGGTTTTCAAGTT2900.10824530625956477No Hit
ACTTACTCTTCAGTGTTTTCATTGCCCAACCACTCGTTCAACTTCTTTTG2830.10563248852226495No Hit
TCAGCCCCACCAGTTTCATCGAATTCTAGCCCAACCTTCTTGTCCATTTT2830.10563248852226495No Hit
TTTTAGCACTTACCTGTGACTCCATAGAAAATCTTTCTCCTGCTCAGTGA2820.10525922884550781No Hit
GGAAAAAGAAGATGTCCCTGTGGAAATGTCCAATGGCGAACCAGGTTGCC2800.10451270949199357No Hit
CCTCTGCGGCCTTCTGGGCCAGAAGTTTTGCCTCCTCCTCGGTGATCTGG2720.10152663207793663No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)