FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000696095

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000696095
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences390680
Sequences flagged as poor quality0
Sequence length108
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGCCCCACCAGTTTCATCGAGTTCTAGCCCAACCTTCTTGTCCATTTT270556.925104945223713No Hit
GACAGGGAAAGATCTGCTGGACCCATCTGCAGTACACACATGTCCTACCT267286.841404730213986No Hit
GGAAAAAGAAGATGTCCCTGTGGAAATGTCCAATGGTGAACCAGGTTGCC240426.1538855329169655No Hit
GAACAAAGGAACTGATGCCCTCTAGCAGTCTGGTTACCTTTCTTTTCAAT217895.577198730418758No Hit
CCTTTGCATCTCTTTCTCGTTGCCCTCACGCCGGAAGCGCTCCAGGTATT213795.472253506706256No Hit
CTCAGAAGGAGCCATCACCTTTGCTGGAAAAGAAGATCCAGTTGCTAGAA178084.558206204566397No Hit
AGCCCTTACAACAAAAACAAGAGCTTACAGTAATGTCCGGGATGGGGCGG149683.83126855738712No Hit
TGCTCCTTTAGTGATCAACCCACCGAGCTGTTTACGTTTGACGTCTTCTG114282.9251561380157676No Hit
TGGCTGTGACCTACCTTGTAAGACACCTTCACCACGGAGGGGGCCCACTT96152.461093478038292No Hit
ACTTCAGGAGAAGGATCAAAGAGTAAGCGCTTCATCGCCTCTGCTGTGCA85212.181068905498106No Hit
TGGGATTTGTACCTTCGAACAAGAAATGAGTTTGTTCAGAAAGGAGAAGC76991.9706665301525546No Hit
GAAGACGCGACGTCCATTTGTCGAAAGAACAGGAGAGCCGCCTACCCTCT59971.535015869765537No Hit
CATTGATGCGTTTGTGCTCCATGAGGTACGTGATGAGCGCGATGGTCTGG56051.4346779973379749No Hit
CATCCTGCTCCTCGTGCTCCAGGATGGCCTGCAGGAAGGCGCGCCGCTCA47341.2117333879389782No Hit
CCAGCCCCACCAGTTTCATCGAGTTCTAGCCCAACCTTCTTGTCCATTTT42091.0773523087949217No Hit
CAAAAATAGGCTGTCCGTCAACATTGAGAGATGGCACAAATAATTCAGTT37120.9501382205385482No Hit
GACAGTAAGATACAGTCTATCGGGTTTAAGTTATACAACATAGTACAGTA36560.9358042387631821No Hit
TTCTAGCAATTTCTTTGTCTTTCACAGAACAAAACTGAAGAATCAAGAAT30820.7888809255656803No Hit
ACACACCTTTTGTGTTTCATCCTTCTTCTCCTGTTTGAGAATGTCAGTTA27760.710555953721716No Hit
CGCCCTTACAACAAAAACAAGAGCTTACAGTAATGTCCGGGATGGGGCGG26060.6670420804750691No Hit
GGCTCTGAGAAGCCATAGTGCCCATCTCGGTGGAAGACCTTGATCAGCTT25910.6632026210709532No Hit
CGCTCCTTTAGTGATCAACCCACCGAGCTGTTTACGTTTGACGTCTTCTG21340.5462270912255554No Hit
CCTTCAGGAGAAGGATCAAAGAGTAAGCGCTTCATCGCCTCTGCTGTGCA15700.40186341763079764No Hit
CGGCTGTGACCTACCTTGTAAGACACCTTCACCACGGAGGGGGCCCACTT15550.39802395822668174No Hit
CTTGAAAGCCAGGAAATGCACTCTTACCTCTTCCTCTTCTTCTGAGCCAC15130.38727347189515715No Hit
GTCTTCTTCAGGGCCGTACTCTTCGACGCCTGCCTCCTCCGCGCCTACCT15070.38573768813351084No Hit
TGTTCAATGAGGTTCCCAGAAGAGAAAAAACAAACCTGGAGCTAAAGAAA14230.36423671547046177No Hit
CGGGATTTGTACCTTCGAACAAGAAATGAGTTTGTTCAGAAAGGAGAAGC13750.35195044537729087No Hit
CTGCAGGCCAACTGAAATACCAGAAAATGACATTCTGCTTTGTGAGAGCC11830.30280536500460736No Hit
TTTTAGCACTTACCTGTGACTCCATAGAAAATCTTTCTCCTGCTCAGTGA11700.29947783352104024No Hit
CCTCTGCGGCCTTCTGGGCCAGAAGTTTTGCCTCCTCCTCGGTGATCTGG11700.29947783352104024No Hit
AGCCCTTACAACAAAAACAAGAGCTTACAATAATGTCCGGGATGGGGCGG9920.2539162485921982No Hit
CAGCCCCACCAGTTTCATCGAGTTCTAGCCCAACCTTCTTGTCCATTTTG9270.23727859117436265No Hit
CCACCCCACCATCTTCCGCCAGGTGTGCCTGGCCTCCCGGGCATCCCACC9070.2321593119688748No Hit
GCACTTTGTGGTTGGTTGCTCGGAGTTTGGGAAGAATTCTATCAAGAAGC8710.22294460939899663No Hit
CCGCAATGACAAATATGCTGGTGAAGAGGGAATGATAGAAGACATGAAGC8570.21936111395515512No Hit
CCTTTGCATCTCTTTCTCGTTGCCCTCACGCCGGAAGCGCTCCAGGCATT8470.21680147435241118No Hit
CGACATCAGCTCGCCCTCCGTGAACATGCCCAGCTGCTGCGGGTCCACCA7740.19811610525238046No Hit
GGCCACTGTGTCCTTGATTGTGTACTGCAGTCCAAAGAACCAGGTTTCTC7460.19094911436469744No Hit
CCTCCCTGGCTTGTACTGCACCAGATCAGGGTGGGGTGGGGCAGGGCAGA7440.19043718644414867No Hit
TGTTCAATGAGGTTCCCAGAAGAGAAAAAACAAACCTGGAGCTAAAGAGA7060.1807105559537217No Hit
GAACCAGCTCCTCTTCAGCATTCTCAGGATAAAATTTGGCCAAGAAGTGA6320.1617692228934166No Hit
ACTTACTCTTCAGTGTTTTCATTGCCCAACCACTCGTTCAACTTCTTTTG5950.15229855636326406No Hit
CTCTAGCAATTTCTTTGTCTTTCACAGAACAAAACTGAAGAATCAAGAAT5760.1474352411180506No Hit
TGGGTCCCAGTCTGCAGTTAAGGGGGCAGGAGTGGCGCTGCTCACCTCTG5510.14103614211119075No Hit
TGAAATACACTTACCAGGATGATTGGAAATGGGAGGCTGGAATGCAAGCT5270.1348930070646053No Hit
GGAAAAAGAAGATGTCCCTGTGGAAATGTCCAATGGCGAACCAGGTTGCC5100.1305416197399406No Hit
CCACACCTTTTGTGTTTCATCCTTCTTCTCCTGTTTGAGAATGTCAGTTA5080.13002969181939183No Hit
CCCACGTTCTAAGAGAGTGACAGAAAGGTAAAGAGGAGCAGCCGCAGAAA4930.12619023241527594No Hit
GCAGCCAGAACTCTTTATTTTGTGTTTTTAATTGCTCGAGCTCACCTCTC4890.12516637657417837No Hit
CCTTTGCATCTCTTTCTCGTTGCCCTCACGCCGGAAGCGCTCCAGGGATT4670.11953516944814169No Hit
GGCTCTGACCATTCTGTTCTCTCTGGCAGGTCATGATGATGGGCAGCGCC4650.11902324152759292No Hit
GGCAGCACCATCACCACATAGAGAGTACCTGGGAACAACCTACTTTGAGG4610.11799938568649534No Hit
TTCTCCACATTACCAGTCTTCTCCAGCTTACTTGAAGGAGATCCTGGAGC4540.11620763796457458No Hit
TCTTGCTTCTTTAAATAGTTCATGCTTTATGGTGTAGAACCATGTCGTCA4110.10520118767277567No Hit
GAGGTTTGAGAGAGCTTGAAGAAATGTTGTAGAACCATGTCGTCAGTGTA4090.10468925975222688No Hit
ACATAAGAGAGAAGGTTTGACTGCCATTGTAGAACCATGTCGTCAGTGTA4050.10366540391112931No Hit
GGTCCCAACACCGGAAAGGATATATTTTCTGCTTCTCTTCGTTGTATAAC3980.10187365618920854No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)