FastQCFastQC Report
Mon 12 Sep 2022
EGAF00000709917

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000709917
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3607516
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTCAGGGAGTTAAGCTTCCCGTTTTTCTAGGTGAGTGTCCCAAAGTTA145663040.37764489471426No Hit
TTGTCAGGGAGTTAAGCTTCAGAACACCTTGGAATACAGTTTAGCTTCCT407411.1293366405027725No Hit
TTGTCAGGGAGTTAAGCTTGATTAGGTTAGGTATTTTTAAAGAAAACTCA402081.1145619312568538No Hit
TTGTCAGGGAGTTAAGCTTTTATCACAATGAAAGAAATACTCAAAGCATG289170.8015764864244539No Hit
TTGTCAGGGAGTTAAGCTTTCTTAGCACAGAAGGAGTAAACTGCAAATGA262970.7289503359098061No Hit
TTGTCAGGGAGTTAAGCTTTCCTAGGGTGCTACACAGATATATAAAGCTG241620.6697683392118011No Hit
TTGTCAGGGAGTTAAGCTTGTTTTCAGCCAGTGTAAGCGTGGTGTTCTTG229090.6350352985267425No Hit
TTGTCAGGGAGTTAAGCTTGCAAAAGGAGATCATAAGACAATTATTGGAT190020.5267336305646323No Hit
TTGTCAGGGAGTTAAGCTTGAGCTCCACCGAAACCAGCACGGAATTTCTA181990.504474547029036No Hit
TTGTCAGGGAGTTAAGCTTGTCTATGAACTGAAGGAAGAAAGATGGGACC161440.4475101427131578No Hit
TTGTCAGGGAGTTAAGCTTGTAAAGAATTTTGTGAGCTGAAGTATTTTAA154940.4294922046083787No Hit
TTGTCAGGGAGTTAAGCTTATTTTAAAAAACGGATTATTGAAACCCAGAA154010.42691425346415646No Hit
TTGTCAGGGAGTTAAGCTTTTTTTATTTAAAAAAACCCAATAACTCAGTT115090.31902838407369505No Hit
TTGTCAGGGAGTTAAGCTTTGGAGGACAAGATGTGCTGATCATTATCTTA111330.3086056998776998No Hit
TTGTCAGGGAGTTAAGCTTAAGGATATAGCCATCCACTGTTAATTAACCT107740.29865425406290647No Hit
TTGTCAGGGAGTTAAGCTTTACCTTTAAAGAGTCCGATGAACTAAAGGCA98700.27359546014487535No Hit
TTGTCAGGGAGTTAAGCTTATTTTGAGGGTAGCAACATGTCATATGTAAG98270.27240350423948223No Hit
TTGTCAGGGAGTTAAGCTTTGTGGAAAATGGTCTTTCAGCAGCAGCATCT85830.23791994269741287No Hit
TTGTCAGGGAGTTAAGCTTTGAACCTAAGTTGAACCTTCCAACCCCGGCA84590.23448267450511653No Hit
TTGTCAGGGAGTTAAGCTTCCCGTTTTTCTAGGGGAGTGTCCCAAAGTTA77740.2154945397331571No Hit
TTGTCAGGGAGTTAAGCTTCTTGCAGCACCTTTTTTTCACTGTCGCATCA72150.19999911296304712No Hit
TTGTCAGGGAGTTAAGCTTAATTTTTCCAACAGCCCTGCTCATAAAAGGA72070.1997773537248345No Hit
TTGTCAGGGAGTTAAGCTTAATGCAGAAAATATACCCAAGTCAGGAGGTA68660.19032486619601965No Hit
TTGTCAGGGAGTTAAGCTTCCCGTTTTTCTAGGTGGGTGTCCCAAAGTTA67380.186776718384617No Hit
TTGTCAGGGAGTTAAGCTTTATGATATTGGGCAAGTGAATAACTTCTAGC67040.18583424162221318No Hit
TTGTCAGGGAGTTAAGCTTCCCGTTTTTCTAGGTGAGTGTCCCAAGGTTA66910.1854738828601176No Hit
TTGTCAGGGAGTTAAGCTTCCCGTTTTTCTAGGCGAGTGTCCCAAAGTTA63960.17729651095102558No Hit
TTGTCAGGGAGTTAAGCTTTTTCCTTCGCAAGTACTGCTACATCTACATC63570.17621543466473885No Hit
TTGTCAGGGAGTTAAGCTTGTATTGTTTCTTGGACCCATCCCCTACACTC60080.16654118789771133No Hit
TTGTCAGGGAGTTAAGCTTATACATAGTAGAGCAAGGAATTGAACCTAAA59680.165432391706648No Hit
TTGTCAGGGAGTTAAGCTTCTGCACTTTCAACAAAACTGTAACCAGGGGC59410.16468395427768026No Hit
TTGTCAGGGAGTTAAGCTTTAATCAGTAATCTTCAAGTAGGAAGTTATTC57970.1606922879898523No Hit
TTGTCAGGGAGTTAAGCTTCCCGTTCTTCTAGGTGAGTGTCCCAAAGTTA56540.1567283416068009No Hit
TTGTCAGGGAGTTAAGCTTCCCGTTTTCCTAGGTGAGTGTCCCAAAGTTA56340.15617394351126926No Hit
TTGTCAGGGAGTTAAGCTTCCAACTAATGAGAATGAATCTCATCAATGTC55670.1543167098912382No Hit
TTGTCAGGGAGTTAAGCTTCATAAAAGTATAAGCAATTCCAGATTCAAAA55100.15273667531897295No Hit
TTGTCAGGGAGTTAAGCTTCCCGTTTTTCTAGGTGAGTGCCCCAAAGTTA54070.14988152512698488No Hit
TTGTCAGGGAGTTAAGCTTCTAGGGAAAGACTAAATGTTCAAATTATAAT52960.14680461569678416No Hit
TTGTCAGGGAGTTAAGCTTCCCGCTTTTCTAGGTGAGTGTCCCAAAGTTA51700.14331190769493468No Hit
TTGTCAGGGAGTTAAGCTTCAGGCATCACGATATTCCCATATGTCTACTG51410.14250803045641378No Hit
TTGTCAGGGAGTTAAGCTTCCCGTTTCTCTAGGTGAGTGTCCCAAAGTTA49500.13721352864408642No Hit
TTGTCAGGGAGTTAAGCTTCCCGTTTTTCTAGGTGAGCGTCCCAAAGTTA49190.13635421159601233No Hit
TTGTCAGGGAGTTAAGCTTCCCGTCTTTCTAGGTGAGTGTCCCAAAGTTA47980.13300010311804578No Hit
TTGTCAGGGAGTTAAGCTTCTCCACAGTGAAGGAAACAATAAACAGACTA47870.13269518416550335No Hit
TTGTCAGGGAGTTAAGCTTATCCACCAGGATCAAGTGGGCTTCATCCCTG47620.13200218654608878No Hit
TTGTCAGGGAGTTAAGCTTCCCGTTTTTCTAGGTGAGTGTCCCAGAGTTA46910.13003407330695138No Hit
TTGTCAGGGAGTTAAGCTTATCCCTTTTCCCTGAAAAACCTCTGTTGTAC46610.12920247616365388No Hit
TTGTCAGGGAGTTAAGCTTTTAACATAGGGAGCCCCTTATTCACCATAAG46300.1283431591155798No Hit
TTGTCAGGGAGTTAAGCTTTAAACCTTTTGGAATGTAAAGGCATATTTGT46210.12809367997259055No Hit
TTGTCAGGGAGTTAAGCTTCACTCATTTTTTCAACCAGTTGGCTGCAATT44680.12385253454177333No Hit
TTGTCAGGGAGTTAAGCTTCCCGTTTTTCTAGGTGAGTGTCCCGAAGTTA43780.12135774311188086No Hit
TTGTCAGGGAGTTAAGCTTCCCGTTTTTCTGGGTGAGTGTCCCAAAGTTA43010.11922331044408396No Hit
TTGTCAGGGAGTTAAGCTTTGTTGGGAGGATGCAGTGAGGTGATATATGG42380.11747695644315921No Hit
TTGTCAGGGAGTTAAGCTTCACGTTTTTCTAGGTGAGTGTCCCAAAGTTA42260.11714431758584024No Hit
TTGTCAGGGAGTTAAGCTTTATATTATTTCTTTCAGTCCTCACAAGAGTC41520.11509304463237309No Hit
TTGTCAGGGAGTTAAGCTTGGGCCTGTGATGTTCATCTCAGCCCCATGTC41290.11445548682251167No Hit
TTGTCAGGGAGTTAAGCTTCCCGTTTTTCTAGGTGAGTGTCCCAAAGCTA41200.11420600767952241No Hit
TTGTCAGGGAGTTAAGCTTACCTCATTTTGGTTTTGTCAATAAAGGGTCA40900.11337441053622492No Hit
TTGTCAGGGAGTTAAGCTTCATGTCATATGTAAGCACAATTTGGGGGGGT40480.11221017453560843No Hit
TTGTCAGGGAGTTAAGCTTAGTCCTGAGACCCGGGGAGATTAAAAAAAAA40400.11198841529739577No Hit
TTGTCAGGGAGTTAAGCTTCATAAGTGAAGGAGAAATAAAATCCTTTACA40160.11132313758275778No Hit
TTGTCAGGGAGTTAAGCTTTCAAACAGAATTTAAAGAGTTAACGCTGGCC39880.11054698024901344No Hit
TTGTCAGGGAGTTAAGCTTGATGCAGTGGTTCACACCTGTAATCCCATCA39800.11032522101080079No Hit
TTGTCAGGGAGTTAAGCTTTAGAATTGTAGTTCCAATGCTATCAAGGAAG39540.10960450348660962No Hit
TTGTCAGGGAGTTAAGCTTCTGGTGTACTGAATCTTTTCCATTTAACAAA38550.1068602329137279No Hit
TTGTCAGGGAGTTAAGCTTTTTTTCTTGTCTTTTTGTATGTTTCATAATA37220.10317348557844234No Hit
TTGTCAGGGAGTTAAGCTTTTTAGCTGAAGGAGGCTTTGAGCCAAATTTA37040.10267452729246385No Hit
TTGTCAGGGAGTTAAGCTTCTGAAACACCTTATTTCAGCTTATCTTCAAT36870.10220328891126192No Hit
TTGTCAGGGAGTTAAGCTTACTAAAGGTCAGAAAATTATCCATTTTACCC36810.10203696948260244No Hit
TTGTCAGGGAGTTAAGCTTTTCTGCCAGACTACTCAGAAGTAAAGTGATT36730.10181521024438976No Hit
TTGTCAGGGAGTTAAGCTTTAGTTGGCAAGGCAGTAGCAATTCTGGGTGC36400.10090045338676253No Hit
TTGTCAGGGAGTTAAGCTTTACCTAAAACCTTGAGATATTGTTAAATAAA36310.10065097424377328No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)