FastQCFastQC Report
Sun 11 Sep 2022
EGAF00000709918

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000709918
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2373728
Sequences flagged as poor quality0
Sequence length101
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAACCTAATCAAGCTTTTATCACAATGAAAGAAATACTCAAAGCATG53556922.562357607948343No Hit
ACCTAACCTAATCAAGCTTGCAAAAGGAGATCCCTATTTTACTTTTACGT697202.9371520241577804No Hit
ACCTAACCTAATCAAGCTTCCCGTTTTTCTAGGTGAGTGTCCCAAAGTTA658802.775381172569056No Hit
ACCTAACCTAATCAAGCTTTCCTAGGGTGCTACACAGATATATAAAGCTG453541.9106654174362014No Hit
ACCTAACCTAATCAAGCTTGTAAAGAATTTTGTGAGCTGAAGTATTTTAA278011.1711956888068051No Hit
ACCTAACCTAATCAAGCTTTGGAGGACAAGATGTGCTGATCCCTATTTTA270721.1404845037005082No Hit
ACCTAACCTAATCAAGCTTGAGCTCCACCGAAACCAGCACGGAATTTCTA238561.0050014154949514No Hit
ACCTAACCTAATCAAGCTTAACTCCCTGACAAAACTAGGAATTCCCTGAA220470.9287921783793257No Hit
ACCTAACCTAATCAAGCTTTTCTGCTTCTTAGGGGGCTTTAATCTAAAGA120350.507008385122474No Hit
ACCTAACCTAATCAAGCTTCAAATGGGAAGGATCCCTATTTTACTTTTAC119960.505365399911026No Hit
ACCTAACCTAATCAAGCTTACCTCATTTTGGTTTTGTCAATAAAGGGTCA109600.46172097224281805No Hit
ACCTAACCTAATCAAGCTTGATCCCTATTTTACTTTTACGTGCTTAATCC103630.43657066015988355No Hit
ACCTAACCTAATCAAGCTTGTTTTCAGCCAGTGTAAGCGTGGTGTTCTTG101980.4296195688806805No Hit
ACCTAACCTAATCAAGCTTCTTGCAGCACCTTTTTTTCACTGTCGCATCA98260.4139480176330228No Hit
ACCTAACCTAATCAAGCTTATTTTGAGGGTAGCAACATGGCGTTCCCAAC92830.3910726081505548No Hit
ACCTAACCTAATCAAGCTTCCAACTAATGAGAATGAATCTCATCAATGTC90120.379655967322288No Hit
ACCTAACCTAATCAAGCTTATCCACCAGGATCCCTATTTTACTTTTACGT80490.33908687094730317No Hit
ACCTAACCTAATCAAGCTTGCTATGATCCCTATTTTACTTTTACGTGCTT74660.31452634842745253No Hit
ACCTAACCTAATCAAGCTTATACATAGTAGAGCAAGGAATTGAACCTAAA73710.31052420496366895No Hit
ACCTAACCTAATCAAGCTTTTAGATGTCTCTGTTTACTTTGATCCCTATT70510.2970433006646086No Hit
ACCTAACCTAATCAAGCTTGCATGTCTGCCTTCTTCCTCATAGAAACACT69440.29253562328961025No Hit
ACCTAACCTAATCAAGCTTCCTAAATCATTAAAAGGATATCCTTTTAAAG67340.2836887798433519No Hit
ACCTAACCTAATCAAGCTTTTTTTATTTAAAAAAACCCAATAACTCAGTT66360.2795602529017646No Hit
ACCTAACCTAATCAAGCTTGAGAAAGTTTCTGCATTCCCTTGTTAATTTC62470.2631725286132194No Hit
ACCTAACCTAATCAAGCTTTAGTGTGCTCAGAATGACCAGAGAACCTTGT60970.25685335472303483No Hit
ACCTAACCTAATCAAGCTTGATTAGGTCATCTTTCGGAGACATCGTTCGT59810.2519665269146254No Hit
ACCTAACCTAATCAAGCTTAGTCCTGAGACCCGGGGAGATTAAAAAAAAA58900.24813289475458014No Hit
ACCTAACCTAATCAAGCTTAGTTTGGCTGGATATGAAATTCTGGGTTGAA58130.24488905215761872No Hit
ACCTAACCTAATCAAGCTTGAACAAAGCACAGTGGATATTGAAAGAAAGA57730.24320393912023616No Hit
ACCTAACCTAATCAAGCTTGATGCAGTGGTTCACACCTGTAATCCCATCA56400.2376009382709392No Hit
ACCTAACCTAATCAAGCTTTGAACCTAAGTTGAACCTTCCAACCCCGGCA51560.2172110705186104No Hit
ACCTAACCTAATCAAGCTTCATAGAAACCTAAAGGAGTTAGAGAAGGAAG50990.21480978444034024No Hit
ACCTAACCTAATCAAGCTTGCCTTTGTCAGCTGATCCCTATTTTACTTTT50880.21434637835506007No Hit
ACCTAACCTAATCAAGCTTGATTATGGGTGATGCTAGGCCCAATTTTCAC50550.21295616009921947No Hit
ACCTAACCTAATCAAGCTTCAGAACACCTTGGAATACAGTTTAGCTTCCT49930.21034423489127652No Hit
ACCTAACCTAATCAAGCTTGAGGAATAACCAATGATCCCTATTTTACTTT48760.20541527925693254No Hit
ACCTAACCTAATCAAGCTTAGCGTAGCACTATTTGTGGTCAGATCCCTAT47750.20116036883754163No Hit
ACCTAACCTAATCAAGCTTAGAGAGCACTGAACGGGATAAATGGCCAAAG46620.19639992450693594No Hit
ACCTAACCTAATCAAGCTTCCCAGTCACTTTGTTTACGTACTCAAGCCTC46300.1950518340770299No Hit
ACCTAACCTAATCAAGCTTCAGTAGCCAATTCGATAAAGTGGAAGAAAGG45830.1930718262581054No Hit
ACCTAACCTAATCAAGCTTCCAGAGAAAGGATCCCTATTTTACTTTTACG45620.19218714191347955No Hit
ACCTAACCTAATCAAGCTTAGCAACATATTCTGAGTCATCATGGGAGAAT45450.19147096887259196No Hit
ACCTAACCTAATCAAGCTTAATGCAGAAAATATACCCAAGTCAGGAGGTA45070.18987011148707855No Hit
ACCTAACCTAATCAAGCTTGCAGGTACCACCGCTGGGCTTGGCAAGTGGA43520.1833402984672212No Hit
ACCTAACCTAATCAAGCTTTAAACCTTTTGGAATGTAAAGGCATATTTGT43390.18279263673007187No Hit
ACCTAACCTAATCAAGCTTGTGTATGTGTCACGTAGTTCTCGTGCCAGGG43290.18237135847072622No Hit
ACCTAACCTAATCAAGCTTGTGCTGTTTTTCTGATTTTAAAATTAGAATA43110.18161305760390406No Hit
ACCTAACCTAATCAAGCTTTACAGTGGCTCCATTTCACTCAGGCTCACCA41830.1762206958842799No Hit
ACCTAACCTAATCAAGCTTTCTTAGCACAGAAGGAGTAAACTGCAAATGA41750.1758836732768034No Hit
ACCTAACCTAATCAAGCTTTGGAGTCATATCTGGGCCTGTATCTGTCTCT40130.16905896547540408No Hit
ACCTAACCTAATCAAGCTTATCGACCATGATCCCTATTTTACTTTTACGT40120.16901683764946954No Hit
ACCTAACCTAATCAAGCTTCAACTACGAATAAATAGCACCCATCTGCTTG39600.1668261907008722No Hit
ACCTAACCTAATCAAGCTTGCCCAAGGATGATCCCTATTTTACTTTTACG38950.16408788201512559No Hit
ACCTAACCTAATCAAGCTTTAGGCCTGGGGCAAGGTCTGAGTTCCATGTG38830.1635823481039108No Hit
ACCTAACCTAATCAAGCTTAATCAGGCAGGACATTAGAGTCCAGGGGGGC38820.16354022027797624No Hit
ACCTAACCTAATCAAGCTTCTCCACAGTGAAGGAAACAATAAACAGACTA38620.16269766375928496No Hit
ACCTAACCTAATCAAGCTTATCCACCACGATCCCTATTTTACTTTTACGT37770.15911679855484706No Hit
ACCTAACCTAATCAAGCTTTTCCCTCAGAATTCTGATTTTTATTCTGAAA36350.15313464727213902No Hit
ACCTAACCTAATCAAGCTTGCAATTTATCAGATCCCTATTTTACTTTTAC35290.14866909772307527No Hit
ACCTAACCTAATCAAGCTTTACAATACCCTGATATATAGGAATTATACTA34690.14614142816700143No Hit
ACCTAACCTAATCAAGCTTTTTAAAAGCAAGATCCCTATTTTACTTTTAC34600.14576227773359038No Hit
ACCTAACCTAATCAAGCTTTCTATATAGGGATCCCTATTTTACTTTTACG33190.13982225427681688No Hit
ACCTAACCTAATCAAGCTTAAAGTGTCAATTTTTATACTTATCTGGTTTG32520.1369996899392011No Hit
ACCTAACCTAATCAAGCTTTTGCTTTCACTCCAATTAAAACAACAATGGT32350.13628351689831353No Hit
ACCTAACCTAATCAAGCTTCCAAATCAGGTGACTTTCAGTTAATAAAAGA31910.13442989255719273No Hit
ACCTAACCTAATCAAGCTTTCCCCAGGCCAAGGCACCAGCTGCTGCTCCA31910.13442989255719273No Hit
ACCTAACCTAATCAAGCTTTCATGCTATGTTCAGTAAGGCAGAATCAAAT31860.13421925342751992No Hit
ACCTAACCTAATCAAGCTTGGGTACAGCCTGCAGCCAGTGTCCCATCTGA31700.13354520821256688No Hit
ACCTAACCTAATCAAGCTTTTAAAGTATATTAGAGTTGATTGGGTTAGAC31570.13299754647541756No Hit
ACCTAACCTAATCAAGCTTATCCACCATGATCCCTATTTTACTTTTACGT31560.13295541864948301No Hit
ACCTAACCTAATCAAGCTTTAGAAAACTCTTTTTTCTTATCAGAATCTAG31460.1325341403901374No Hit
ACCTAACCTAATCAAGCTTGAGTCTGGGGAGAAGGATGGTGGGAAAGAAA31250.13164945604551154No Hit
ACCTAACCTAATCAAGCTTGCCTGGTTCTTAGGCCTTCAGATCCCTATTT31140.13118604996023134No Hit
ACCTAACCTAATCAAGCTTACCCAGTGTGTTAGGCATAATACTAGCCCTC30900.1301749821378018No Hit
ACCTAACCTAATCAAGCTTTCCCATCAATTCCAGTTCCCAGAACAAAAAA30820.1298379595303253No Hit
ACCTAACCTAATCAAGCTTTGAGGTCTGCACATCAGATAGCATTACTCCC30670.12920604214130682No Hit
ACCTAACCTAATCAAGCTTCACAGCTGCTGATGGACACAGACACTGGGAC30530.12861625257822293No Hit
ACCTAACCTAATCAAGCTTATTCACCATGATCCCTATTTTACTTTTACGT30120.12688901171490585No Hit
ACCTAACCTAATCAAGCTTTTCTTTCAAGGGTGAATAATATTAATACTTG29600.12469836476630852No Hit
ACCTAACCTAATCAAGCTTCTCATTCAGATCCCTATTTTACTTTTACGTG29240.12318176303266423No Hit
ACCTAACCTAATCAAGCTTTCTAGTCTTTCTCAAGGAAACCTGTCCAAAG29000.1221706952102347No Hit
ACCTAACCTAATCAAGCTTCTGCGTCAGCAATTCACAATCAATTTGCTTT28870.12162303347308537No Hit
ACCTAACCTAATCAAGCTTACCTTAAGCCTAAGAGTTATATACAATTATT28630.12061196565065584No Hit
ACCTAACCTAATCAAGCTTTCATCACAATGAAAGAAATACTCAAAGCATG28530.1201906873913102No Hit
ACCTAACCTAATCAAGCTTATTGCAGACATTTCCATCCATTTTAGAGTTC28510.12010643173944108No Hit
ACCTAACCTAATCAAGCTTGGGCCTGTGATGTTCATCTCAGCCCCATGTG27180.11450343089014411No Hit
ACCTAACCTAATCAAGCTTGGATTATCCAGGAGGGAAACCTGACAATATA26960.1135766187195837No Hit
ACCTAACCTAATCAAGCTTAGTCATCTGACTTAGAAAGGACAGAAACTAT26940.11349236306771458No Hit
ACCTAACCTAATCAAGCTTCTCTGTTCCCTGATGCAGTGTGGATATTTTT26940.11349236306771458No Hit
ACCTAACCTAATCAAGCTTTTCTGTTATCCTGACTAGAACTTACCATCTT26890.11328172393804176No Hit
ACCTAACCTAATCAAGCTTCAATATCTGAGGGCAGGAGAAGGTAGATATC26790.11286044567869612No Hit
ACCTAACCTAATCAAGCTTCTGTTGAAGGCAATCCTACTGGAACTTTTAG26650.11227065611561224No Hit
ACCTAACCTAATCAAGCTTTCAGGACCCCTCACTTACACTGACACCTTCT25980.10944809177799647No Hit
ACCTAACCTAATCAAGCTTTCAAACAGAATTTAAAGAGTTAACGCTGGCC25720.1083527683036978No Hit
ACCTAACCTAATCAAGCTTTTCTCTCACTATCAACTTTTCTTTCCACTTG25630.10797361787028674No Hit
ACCTAACCTAATCAAGCTTGCTTTCCTAGCTAGCTGCCCCCTCCACTACC25580.10776297874061393No Hit
ACCTAACCTAATCAAGCTTAGTTATTGAGAAGCTTCTTGCAGCACCTTTT25420.10708893352566089No Hit
ACCTAACCTAATCAAGCTTTTAGTTGGAAAGATTGTTCAATGATCCCTAT25010.1053616926623438No Hit
ACCTAACCTAATCAAGCTTTTATCACAATGAAGGAAATACTCAAAGCATG24960.10515105353267097No Hit
ACCTAACCTAATCAAGCTTCATAAGTGAAGGAGAAATAAAATCCTTTACA24610.10367657962496125No Hit
ACCTAACCTAATCAAGCTTTCAAAAGTAGGAAGCTGGTCAATGAGAATTT24590.1035923239730921No Hit
ACCTAACCTAATCAAGCTTCTATAGATCCCTATTTTACTTTTACGTGCTT24330.10249700049879346No Hit
ACCTAACCTAATCAAGCTTTTATCACAATGAGAGAAATACTCAAAGCATG24320.10245487267285888No Hit
ACCTAACCTAATCAAGCTTCAATTATAAAATGAATGACAGATGCACAAGA24200.10194933876164414No Hit
ACCTAACCTAATCAAGCTTGTCTATGAACTGAAGGAAGAAAGATGGGACC23910.10072763180954179No Hit
ACCTAACCTAATCAAGCTTTTATCACAATGAAAGAAATACTCAAGGCATG23880.10060124833173809No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)