FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000709919

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000709919
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1868237
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGACCTTTAGTAAGCTTTCACTAGAGCAAAGGAAGTCCCTCAACATAA83436944.66076841428577No Hit
TCTGACCTTTAGTAAGCTTGCATGTCTGCCTTCTTCCTCATAGAAACACT120040.6425308994522644No Hit
TCTGACCTTTAGTAAGCTTTAACTAGAGCAAAGGAAGTCCCTCAACATAA78670.42109218477099No Hit
TCTGACCTTTAGTAAGCTTTTTCAACCAGAGAAACAGTATGCTACAAGAG62750.33587815678631777No Hit
TCTGACCTTTAGTAAGCTTCTATTATAAACATTGTGACTTTGTGCAATTT55090.2948769347786175No Hit
TCTGACCTTTAGTAAGCTTAGTCCTGAGACCCGGGGAGATTAAAAAAAAA50470.2701477382152265No Hit
TCTGACCTTTAGTAAGCTTCCTGAAGATGATGTCTGGTTTGGTTTGTTGT41080.21988644909612648No Hit
TCTGACCTTTAGTAAGCTTTTTCTATATTTTGGATACAAGTCCTTCGTCA40340.21592549553402485No Hit
TCTGACCTTTAGTAAGCTTTTAGTTGGAAAGATTGTTCAATGATCTAATT39040.20896706360060313No Hit
TCTGACCTTTAGTAAGCTTTCACTAGAGCAAAGGAAGTCCCTCAGCATAA38190.2044173196441351No Hit
TCTGACCTTTAGTAAGCTTTCACTAGAGCAAAGGAGGTCCCTCAACATAA37870.2027044748605236No Hit
TCTGACCTTTAGTAAGCTTGCCACTGCAGACTGGTGTTCAGAGAAAGCCA37530.20088457727793635No Hit
TCTGACCTTTAGTAAGCTTTCACTAGAGCAAGGGAAGTCCCTCAACATAA37140.19879704769790985No Hit
TCTGACCTTTAGTAAGCTTCCAACTAATGAGAATGAATCTCATCAATGTC36970.19788709890661624No Hit
TCTGACCTTTAGTAAGCTTTCACTAGGGCAAAGGAAGTCCCTCAACATAA36030.19285561735475745No Hit
TCTGACCTTTAGTAAGCTTTCACTAGAGCGAAGGAAGTCCCTCAACATAA35210.188466452596753No Hit
TCTGACCTTTAGTAAGCTTAGCAAGAATTTTAAATTACTGAGTAAATGAA35010.1873959246069958No Hit
TCTGACCTTTAGTAAGCTTTCCTAGGGTGCTACACAGATATATAAAGCTG34580.18509428942901784No Hit
TCTGACCTTTAGTAAGCTTGCATGTGTTTGAAAAAGATCTAATTAAAAAA34170.1828997070500156No Hit
TCTGACCTTTAGTAAGCTTTCACTAGAGCAAAGGGAGTCCCTCAACATAA33300.17824291029457184No Hit
TCTGACCTTTAGTAAGCTTGCCCAGCACCAAGCAGTCATATAGGCCACAA33140.17738648790276607No Hit
TCTGACCTTTAGTAAGCTTACTGTCCCATGAATTATCATAATATGACAAA32930.17626243351352103No Hit
TCTGACCTTTAGTAAGCTTTTAAAGTATATTAGAGTTGATTGGGTTAGAC32170.17219442715244374No Hit
TCTGACCTTTAGTAAGCTTAATTAACAAGGAGTAACTTGCCCAAGATCTA32120.17192679515500442No Hit
TCTGACCTTTAGTAAGCTTCTGTCCGAAGAGAGCAGTGTGCTCCTCCTCC31840.17042805596934435No Hit
TCTGACCTTTAGTAAGCTTTCACTAGAGCAAAGGAAGTCCCTCAACGTAA31820.17032100317036863No Hit
TCTGACCTTTAGTAAGCTTTAAACCTTTTGGAATGTAAAGGCATATTTGT31730.16983926557497792No Hit
TCTGACCTTTAGTAAGCTTTCTGGATTAAAAAATATATATATGCCACCAA30000.16057919846357824No Hit
TCTGACCTTTAGTAAGCTTAGGCAAATGATGAGACAATATTTCATCAAAA29940.16025804006665106No Hit
TCTGACCTTTAGTAAGCTTGGTGGACCTCCAGAACCATTTGAGGGTACTT29320.15693940329840378No Hit
TCTGACCTTTAGTAAGCTTTCAAACAGAATTTAAAGAGTTAACGCTGGCC29090.15570829611018303No Hit
TCTGACCTTTAGTAAGCTTCCACTAGAGCAAAGGAAGTCCCTCAACATAA28910.15474482091940156No Hit
TCTGACCTTTAGTAAGCTTTCACTAGAGCAAAGGAAGCCCCTCAACATAA28870.1545307153214501No Hit
TCTGACCTTTAGTAAGCTTCCTGAAGAGTCATCAGCAGCTGGAGACCATG28790.15410250412554724No Hit
TCTGACCTTTAGTAAGCTTTCACTAGAGCAGAGGAAGTCCCTCAACATAA28730.1537813457286201No Hit
TCTGACCTTTAGTAAGCTTTCACTAGAGCAAAGGAAGTCCCCCAACATAA28660.15340666093220506No Hit
TCTGACCTTTAGTAAGCTTTAATTCTCACAGAAGAACTGTGATAGTTTTT28280.1513726577516664No Hit
TCTGACCTTTAGTAAGCTTCTGTTGAAGGCAATCCTACTGGAACTTTTAG27920.14944570737010346No Hit
TCTGACCTTTAGTAAGCTTTCACTGGAGCAAAGGAAGTCCCTCAACATAA27290.14607354420236834No Hit
TCTGACCTTTAGTAAGCTTTCACTAGAGCAAAGGAAGTCCCTCAACATAG27250.14585943860441689No Hit
TCTGACCTTTAGTAAGCTTTCAGGACCCCTCACTTACACTGACACCTTCT26800.14345075062746324No Hit
TCTGACCTTTAGTAAGCTTTCACTAGAGCAAAGGAAGTCCCTCGACATAA26700.14291548663258463No Hit
TCTGACCTTTAGTAAGCTTGTGCTGTTTTTCTGATTTTAAAATTAGAATA25940.1388474802715073No Hit
TCTGACCTTTAGTAAGCTTGATCTAATTAAAAAATTTAAAGAAGGATTAT25820.138205163477653No Hit
TCTGACCTTTAGTAAGCTTACCTTAAGCCTAAGAGTTATATACAATTATT25640.13724168828687153No Hit
TCTGACCTTTAGTAAGCTTTCGCTAGAGCAAAGGAAGTCCCTCAACATAA25440.13617116029711435No Hit
TCTGACCTTTAGTAAGCTTAGTTTGGCTGGATATGAAATTCTGGGTTGAA25210.1349400531088936No Hit
TCTGACCTTTAGTAAGCTTAATAATCAACATAGAAGTGTTATGAGCTTTA25070.13419068351606356No Hit
TCTGACCTTTAGTAAGCTTTAAGGCTAAATAAAAATGATATTAACACGCA24950.1335483667222092No Hit
TCTGACCTTTAGTAAGCTTCTGTGTTCCGAATTAGACACATCTTTATTTA24790.13269194433040346No Hit
TCTGACCTTTAGTAAGCTTCCTTTTCAAGTCTCAAGTACATATTTAAAGC24720.13231725953398846No Hit
TCTGACCTTTAGTAAGCTTATCCACCATGATCTAATTAAAAAATTTAAAG24320.1301762035544741No Hit
TCTGACCTTTAGTAAGCTTTAGTGTGCTCAGAATGACCAGAGAACCTTGT24190.12948036036113192No Hit
TCTGACCTTTAGTAAGCTTCTACCACCTCTTCTGGAATGGTTCTAGCTAG23990.1284098323713747No Hit
TCTGACCTTTAGTAAGCTTTCATAGCCTTTAAAATGTCTCTATTCTGTAT23770.12723225158264181No Hit
TCTGACCTTTAGTAAGCTTATCCCAGGCCCAGGTCTCCGAACTATTTGTC23760.12717872518315396No Hit
TCTGACCTTTAGTAAGCTTGGGCTTGTAAAACTTGAGCTGCAAATTTTTA23700.12685756678622678No Hit
TCTGACCTTTAGTAAGCTTTGGTCTGGAACACAGTTATCTGGAAATAGTT23700.12685756678622678No Hit
TCTGACCTTTAGTAAGCTTATCCACCACGATCTAATTAAAAAATTTAAAG23540.12600114439442106No Hit
TCTGACCTTTAGTAAGCTTTGAAACATTAAGTATAGTCTTTATTCATGTT23460.12557293319851817No Hit
TCTGACCTTTAGTAAGCTTTAGAATTGTAGTTCCAATGCTATCAAGGAAG22880.12246840202822233No Hit
TCTGACCTTTAGTAAGCTTATAAAAATTTAAATAGTAATACAAGACAGAA22750.12177255883488015No Hit
TCTGACCTTTAGTAAGCTTACTACTGAAGTTTGAATAAAAACTATTTGTC22640.1211837684405137No Hit
TCTGACCTTTAGTAAGCTTATTTTTGACATATGACAACCATATTTTGGAT22140.11850744846612074No Hit
TCTGACCTTTAGTAAGCTTCTATAGATCTAATTAAAAAATTTAAAGAAGG21990.11770455247380285No Hit
TCTGACCTTTAGTAAGCTTCCCGTTTTTCTAGGTGAGTGTCCCAAAGTTA21860.11700870928046067No Hit
TCTGACCTTTAGTAAGCTTTTTTTATTTAAAAAAACCCAATAACTCAGTT21630.11577760209223992No Hit
TCTGACCTTTAGTAAGCTTCTAGGGAGCAGAGATGATGGTGTAATTCCTT21600.11561702289377633No Hit
TCTGACCTTTAGTAAGCTTCTTGCAGCACCTTTTTTTCACTGTCGCATCA21140.1131548085173348No Hit
TCTGACCTTTAGTAAGCTTTATTAAGTCATAGTTTCTCTTTCAATGGTAC20740.11101375253782042No Hit
TCTGACCTTTAGTAAGCTTCCTTGTGTTTCAAAGTATATCAACCCTAACA20600.11026438294499039No Hit
TCTGACCTTTAGTAAGCTTGAAACCTCTTGCCTGTTTGACTTGTCCACTG20450.1094614869526725No Hit
TCTGACCTTTAGTAAGCTTCTATGTAGCAACTAGTTCATTTCTCCATTCG19640.10512584859415587No Hit
TCTGACCTTTAGTAAGCTTGATTAGGTTAGGTATTTTTAAAGAAAACTCA19630.10507232219466801No Hit
TCTGACCTTTAGTAAGCTTTTCTGCTTCTTAGGGGGCTTTAATCTAAAGA19550.10464411099876515No Hit
TCTGACCTTTAGTAAGCTTGAGTTGAACACAGTTGTACATTTTCTTAACC19300.10330595101156867No Hit
TCTGACCTTTAGTAAGCTTCAGGGCCTCTCACTTGCATAGGCTCTTTTAG19210.10282421341617792No Hit
TCTGACCTTTAGTAAGCTTCCCTCTACAACATCCTCTCTTTCCAGCTGTG19050.10196779102437219No Hit
TCTGACCTTTAGTAAGCTTTCACTAGAGCAAAGGAAGTCCCTCAACATGA19010.10175368542642074No Hit
TCTGACCTTTAGTAAGCTTAGAAAAGAGAACCTAGGAATGAAGACACCAA18860.10095078943410285No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)