FastQCFastQC Report
Tue 13 Sep 2022
EGAF00000709920

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000709920
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7098746
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACAGCCACACCAGCAAGCTTATCTCAGATGATTAAATGGCAGCAGATA93026713.104666655209243No Hit
GAACAGCCACACCAGCAAGCTTGATACGGCGCCCAGCAAAATGTCGTATG2503333.526439740202002No Hit
GAACAGCCACACCAGCAAGCTTAATGATACGGCGCCCAGCAAAATGTCGT2180323.0714157120144883No Hit
GAACAGCCACACCAGCAAGCTTATGATACGGCGCCCAGCAAAATGTCGTA1708662.4069885019128727No Hit
GAACAGCCACACCAGCAAGCTTGAGACGGCGCCCAGCAAAATGTCGTATG1399101.9709114821124745No Hit
GAACAGCCACACCAGCAAGCTTATGAGACGGCGCCCAGCAAAATGTCGTA1052241.4822899706511543No Hit
GAACAGCCACACCAGCAAGCTTAATGAGACGGCGCCCAGCAAAATGTCGT919751.2956513727917578No Hit
GAACAGCCACACCAGCAAGCTTGCTTTGAACTCCAAGTACCTCCAAGGTC788951.1113934771014486No Hit
GAACAGCCACACCAGCAAGCTTATACGGCGCCCAGCAAAATGTCGTATGC668410.9415888383666635No Hit
GAACAGCCACACCAGCAAGCTTAGCTCAGTTAACTCAGCACACAGTTCGT615680.8673081132921223No Hit
GAACAGCCACACCAGCAAGCTTTGTCATTTTGCTCTTAACAATAAACCTT595060.8382607294302402No Hit
GAACAGCCACACCAGCAAGCTTCCATGTGATCTGCCTGCCTCAGCCTCCC569490.8022402830020965No Hit
GAACAGCCACACCAGCAAGCTTCGCTTCTGTGAGCCCCCTTGTCTGTTGG514120.7242405912255488No Hit
GAACAGCCACACCAGCAAGCTTCTCCCTGGGCAGGCTGTGTTGCTAACAC504060.7100690741716917No Hit
GAACAGCCACACCAGCAAGCTTAGACGGCGCCCAGCAAAATGTCGTATGC461410.6499880401411742No Hit
GAACAGCCACACCAGCAAGCTTCATGTGATCTGCCTGCCTCAGCCTCCCA457550.6445504600389984No Hit
GAACAGCCACACCAGCAAGCTTACTGAATCAAGATCTTGCTTGGTGGGGT348160.4904528208221565No Hit
GAACAGCCACACCAGCAAGCTTACGGCGCCCAGCAAAATGTCGTATGCCG325270.4582076890763524No Hit
GAACAGCCACACCAGCAAGCTTATGCTTAAAACTATTTAAATTCAAAAGT311010.43811963408748533No Hit
GAACAGCCACACCAGCAAGCTTGTCCGCATGTGATCTGCCTGCCTCAGCC306530.4318086602901414No Hit
GAACAGCCACACCAGCAAGCTTTAGAGCTGAGCCTTGGAGGAGGGGTGTC295980.4169468804771998No Hit
GAACAGCCACACCAGCAAGCTTTGCCTGGCTGACAAAGGGAGGAGGGGCA291530.41067816766510595No Hit
GAACAGCCACACCAGCAAGCTTCAACTACATCCTAATTACCCAGTGGGAA283890.3999157034214212No Hit
GAACAGCCACACCAGCAAGCTTATCATAGCATCTTAAAGTTGGAAGGGAC241490.3401868442679876No Hit
GAACAGCCACACCAGCAAGCTTCCCAGAATGGTTGCTGTCTCTGAAGGAT239950.33801744702515063No Hit
GAACAGCCACACCAGCAAGCTTCGCTTCTATAATGATGATGAGCTAGTCT239240.3370172703742323No Hit
GAACAGCCACACCAGCAAGCTTATGAAGTGTTACAAGATAAGGGCAACAG231250.32576176130263007No Hit
GAACAGCCACACCAGCAAGCTTTTCTTTAGTAAGACTAATGCTATCAAGG227930.3210848789349555No Hit
GAACAGCCACACCAGCAAGCTTATGACATAAAGGCTTTATTTATTTATTT224260.3159149517393635No Hit
GAACAGCCACACCAGCAAGCTTGTCTTTTATTTTGTTACACATGTGATCT216470.3049411825694285No Hit
GAACAGCCACACCAGCAAGCTTCTTGGCCCCAGGACTGCCCCAGGGGTGG213830.30122221586742226No Hit
GAACAGCCACACCAGCAAGCTTTATCCTACTGTAATAGGCTGCTCCAAAG204200.2876564396021494No Hit
GAACAGCCACACCAGCAAGCTTCACATGTGATCTGCCTGCCTCAGCCTCC202550.28533208541339555No Hit
GAACAGCCACACCAGCAAGCTTGATTCAAAAATCCAACCACATGTGATCT199300.28075381201130456No Hit
GAACAGCCACACCAGCAAGCTTGGCCAGCCTTGGCTGTTTTCTGATCCCG199260.28069746403097107No Hit
GAACAGCCACACCAGCAAGCTTGGTTTCCTCACCTTGAAAGTGGGAATTA195140.274893622056628No Hit
GAACAGCCACACCAGCAAGCTTTGATACGGCGCCCAGCAAAATGTCGTAT193040.27193535308912303No Hit
GAACAGCCACACCAGCAAGCTTATCCACCATGTGATCTGCCTGCCTCAGC186680.26297602421610805No Hit
GAACAGCCACACCAGCAAGCTTCGTCCAGGGTTCTTTATTTTTGTCTGCT183910.25907392657801814No Hit
GAACAGCCACACCAGCAAGCTTTGTGCTTGGGTTTGGGCACTGCAGCTGT183530.25853862076485057No Hit
GAACAGCCACACCAGCAAGCTTGGTGTACATTGACATTTGAATTCGATGT181070.25507321997434473No Hit
GAACAGCCACACCAGCAAGCTTGGCACTATTCCAGACCCCGTGGGGCACT171720.241901879571406No Hit
GAACAGCCACACCAGCAAGCTTTACGGCGCCCAGCAAAATGTCGTATGCC163870.23084358843097078No Hit
GAACAGCCACACCAGCAAGCTTCCAGGGCCCGACTGCCCAGCACAGCCCA162890.22946306291280177No Hit
GAACAGCCACACCAGCAAGCTTTTACCTGGTGAATTTAAGAAGACATAAT162310.22864601719796707No Hit
GAACAGCCACACCAGCAAGCTTCAAGATGGTATCTTTAGGATAATTACAT158850.22377191689912554No Hit
GAACAGCCACACCAGCAAGCTTCTGGAGTGTTTGCCCAGTCTCCGGGGAC156180.22001068921186925No Hit
GAACAGCCACACCAGCAAGCTTCCCAGTCCCTGGATTGGATTCCCACATT153500.21623537452952957No Hit
GAACAGCCACACCAGCAAGCTTTGGGTATATGACAGGAGGCAGCATTTGA149000.20989622674201897No Hit
GAACAGCCACACCAGCAAGCTTCGTGTCCATTTACAGTCACTTTCCATTG148820.2096426608305185No Hit
GAACAGCCACACCAGCAAGCTTTTCATGTGATCTGCCTGCCTCAGCCTCC146320.20612091205967925No Hit
GAACAGCCACACCAGCAAGCTTTTCCATTTTTTCTTGCACAAAGCAGCTG142640.20093689786900393No Hit
GAACAGCCACACCAGCAAGCTTCCAACCCCCTCCCCTCAGTCTCACACCC141660.19955637235083493No Hit
GAACAGCCACACCAGCAAGCTTTGTTCTCTTCATCTGGCCTCAGAGTTAG136280.1919775689959889No Hit
GAACAGCCACACCAGCAAGCTTGCATTCTAGTCCGAGGAGCAGACAGAAT133720.1883712982546495No Hit
GAACAGCCACACCAGCAAGCTTCAGGAAAGAATAGGCTGCACAGGGGCGC126180.17774970396179832No Hit
GAACAGCCACACCAGCAAGCTTGATCAGGAGAATATTGAAGGGAGACTCG125940.17741161607979777No Hit
GAACAGCCACACCAGCAAGCTTGGGGCTTATCCCATTTTTGGCTTGGTGA124410.17525630583204413No Hit
GAACAGCCACACCAGCAAGCTTGTGCAATACATGTGATCTGCCTGCCTCA124410.17525630583204413No Hit
GAACAGCCACACCAGCAAGCTTTGAGACGGCGCCCAGCAAAATGTCGTAT124300.17510134888612722No Hit
GAACAGCCACACCAGCAAGCTTTCATGTGATCTGCCTGCCTCAGCCTCCC120520.16977646474461827No Hit
GAACAGCCACACCAGCAAGCTTTAACCGCTGTCCCTCATGTGATCTGCCT118520.1669590657279469No Hit
GAACAGCCACACCAGCAAGCTTCCTGAGAGCAGAAACTTTTGCTACTTTT118220.16653645587544616No Hit
GAACAGCCACACCAGCAAGCTTGACCACTTCTAGCCAGGGCCTCGGTGGC117670.16576167114586154No Hit
GAACAGCCACACCAGCAAGCTTAGGAGAGGCTTTCTCTGTGAGGGAGGAG117020.16484601646544333No Hit
GAACAGCCACACCAGCAAGCTTAAGCAATGTGGCCTCCCAAAATGCTGGG116930.1647192335096931No Hit
GAACAGCCACACCAGCAAGCTTCTGCCCTATTTCTGGCCCCACGTCCCAA116410.16398670976535856No Hit
GAACAGCCACACCAGCAAGCTTGAAAATACCTGTAGGGAGTAGGAAACTA112980.1591548704517671No Hit
GAACAGCCACACCAGCAAGCTTATAACAAAAGATATAACAGAGAGTTTAT111590.1571967781351805No Hit
GAACAGCCACACCAGCAAGCTTTACCCTGGATGGAGAGTGTATCTCATTT109410.1541258132070087No Hit
GAACAGCCACACCAGCAAGCTTAGGACTCTGATCCTTAGTGCCTGTAATA104260.14687101073907982No Hit
GAACAGCCACACCAGCAAGCTTCTTGACATGTGATCTGCCTGCCTCAGCC104250.14685692374399648No Hit
GAACAGCCACACCAGCAAGCTTCATAAGTGAAGGAGAAATAAAATACTTT103220.1454059632504107No Hit
GAACAGCCACACCAGCAAGCTTAATTAATGCTTACAAAGTGCTCTGAGGC101330.14274352117965625No Hit
GAACAGCCACACCAGCAAGCTTCAGGAGGGAATGGAACAGTGAACTGGTT99550.14023603605481869No Hit
GAACAGCCACACCAGCAAGCTTCAGTCTCCCAAAGTGCTGGGATTACAGG99540.14022194905973534No Hit
GAACAGCCACACCAGCAAGCTTCCTCTCAATGGCAGGGGATACTCAGGGT98180.1383061177283988No Hit
GAACAGCCACACCAGCAAGCTTGAGAAGGGAGGCTGGTCTTGGGAGAGGC98160.13827794373823207No Hit
GAACAGCCACACCAGCAAGCTTGTACCCACGCTGTGCCACTCAGAAGGAA97860.13785533388573137No Hit
GAACAGCCACACCAGCAAGCTTTCAAAAGGAGTACAAGTTTCATAGAGCA94830.1335869743754742No Hit
GAACAGCCACACCAGCAAGCTTCCTTCCTTAGCTTTAGTATGTCCTATTC91090.1283184382142987No Hit
GAACAGCCACACCAGCAAGCTTGGGCCAGACCCACACAGCCCCAGGGAGC91090.1283184382142987No Hit
GAACAGCCACACCAGCAAGCTTCACCACACCCTTGATGGTGGGAGCTCGT90980.12816348126838176No Hit
GAACAGCCACACCAGCAAGCTTCTAGAAAATTCCAACTAAGAAACTGGAG90760.1278535673765479No Hit
GAACAGCCACACCAGCAAGCTTAAACAAGCCCTGAAAGGATCAATCTGAA89990.1267688687551294No Hit
GAACAGCCACACCAGCAAGCTTTGAATCCTGAACTCCCAGCCCCAGCATT89210.1256700831386276No Hit
GAACAGCCACACCAGCAAGCTTGGAGACCAGTCCTAGACCTACACGCTAC89050.12544469121729387No Hit
GAACAGCCACACCAGCAAGCTTCTGGAGGCCCTCAGCAGAAGCGAATGCC88640.12486712441887624No Hit
GAACAGCCACACCAGCAAGCTTCTACCTAAGGTCAACATTATTCTCCCAT88530.12471216747295931No Hit
GAACAGCCACACCAGCAAGCTTCTGTGGGTCTCCCCACAGTGGCCTGGAG87760.12362746885154081No Hit
GAACAGCCACACCAGCAAGCTTGGAGGCTGAAGCAGCTCCACTGTGAATG87110.12271181417112262No Hit
GAACAGCCACACCAGCAAGCTTTCCCCCGAATACTCTTGGGAGATGCAGG84450.11896467347894965No Hit
GAACAGCCACACCAGCAAGCTTCAGTGGCCTGCTCCATCCCCTAATGACT82320.11596414352619462No Hit
GAACAGCCACACCAGCAAGCTTCTACTTACAATTGAAAATTGACATGTGA82170.11575283859994429No Hit
GAACAGCCACACCAGCAAGCTTGCTAGGCAAGGGCAATGGGCCTCCCAGC80090.11282274362260601No Hit
GAACAGCCACACCAGCAAGCTTTGTTTATTATTCAAGAGACTGTTCAAAT79400.11185074096185439No Hit
GAACAGCCACACCAGCAAGCTTGGGATGCACTGTCTTGAAGAATGAAAAG79360.11179439298152095No Hit
GAACAGCCACACCAGCAAGCTTACAAGTTCGAGACCAGCCTGGGTAACAT77640.10937142982718356No Hit
GAACAGCCACACCAGCAAGCTTTAAAAAGCAGCGTGCAACGCAATTTCCC77020.10849803613201543No Hit
GAACAGCCACACCAGCAAGCTTCCAATCATGTGATCTGCCTGCCTCAGCC73960.1041874156365082No Hit
GAACAGCCACACCAGCAAGCTTTTTGGGGGGATGATGGAAATTTATTTAA73660.10376480578400749No Hit
GAACAGCCACACCAGCAAGCTTCCCTTCACAGCCCTGCTGCAACCTCTCA73190.10310271701508972No Hit
GAACAGCCACACCAGCAAGCTTCAGGGTGGATTATTACCTGACACAAAAA71790.10113053770341973No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)