FastQCFastQC Report
Sun 11 Sep 2022
EGAF00000709921

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000709921
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3242681
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGGTTCTCGCAAGCTTCAGGAATACAAAGCTAAAAGACAAGGTATCCC223629268.96429220142221No Hit
CGAGGTTCTCGCAAGCTTCACAGTCTGGGGAGGTGAAAGGGAAGCAAACC246750.7609444160557267No Hit
CGAGGTTCTCGCAAGCTTACAAATTTCTAGGCAAAAAGCACACTTCAGGA176110.5430999842414348No Hit
CGAGGTTCTCGCAAGCTTTCCTTTTATCACCTTTATTTTTTACCTTCCCT152710.4709374742689768No Hit
CGAGGTTCTCGCAAGCTTACAAGCAGAGAAGGAGCAGAGTGTCCGCAATC112290.34628753182937205No Hit
CGAGGTTCTCGCAAGCTTCAGGAATACAAAGCTAAAGGACAAGGTATCCC101710.3136602089443889No Hit
CGAGGTTCTCGCAAGCTTCAGGAATACAAAGCTAAAAGACAAGGAATCCC101650.31347517686753645No Hit
CGAGGTTCTCGCAAGCTTCAGGAGTACAAAGCTAAAAGACAAGGTATCCC94970.292874938977963No Hit
CGAGGTTCTCGCAAGCTTCAGGAATACAAAGCTAAAAGGCAAGGTATCCC93880.2895135229151434No Hit
CGAGGTTCTCGCAAGCTTCAGGAATACGAAGCTAAAAGACAAGGTATCCC85640.264102451027406No Hit
CGAGGTTCTCGCAAGCTTCAGGGATACAAAGCTAAAAGACAAGGTATCCC84980.262067098182029No Hit
CGAGGTTCTCGCAAGCTTCAGGAATACAAAGCTAAAAGACGAGGTATCCC83260.2567628453122586No Hit
CGAGGTTCTCGCAAGCTTCAGGAATACAAAGCTAAAAGACAGGGTATCCC83080.25620774908170124No Hit
CGAGGTTCTCGCAAGCTTCGGGAATACAAAGCTAAAAGACAAGGTATCCC80570.24846724053337346No Hit
CGAGGTTCTCGCAAGCTTCAGGAATACAAAGCTAAGAGACAAGGTATCCC78670.24260789143304567No Hit
CGAGGTTCTCGCAAGCTTCAGGAATACAGAGCTAAAAGACAAGGTATCCC78510.24211447256143911No Hit
CGAGGTTCTCGCAAGCTTCAGGAATACAAGGCTAAAAGACAAGGTATCCC78380.2417135697282588No Hit
CGAGGTTCTCGCAAGCTTCAGGAATACAAAGCTAAAAGACAAGGTACCCC73870.22780532528484917No Hit
CGAGGTTCTCGCAAGCTTGTGCCACCATGCCCAGCCAATTTTGTGTGTGT72610.22391965167094757No Hit
CGAGGTTCTCGCAAGCTTCAGGAATACAAAGCTAAAAGACAAGGCATCCC71170.21947888182648864No Hit
CGAGGTTCTCGCAAGCTTTTCACATTTTTTATATCAAGAAATAAAGCAAG65030.20054393262858727No Hit
CGAGGTTCTCGCAAGCTTCAGGAATACAAAGCTAGAAGACAAGGTATCCC57610.1776616324578335No Hit
CGAGGTTCTCGCAAGCTTCAGGAATACAAAGCTGAAAGACAAGGTATCCC50830.15675300777350595No Hit
CGAGGTTCTCGCAAGCTTATCCACCAAGATCAATCAATGTGAGGAATGAC50080.15444010681285023No Hit
CGAGGTTCTCGCAAGCTTCAGGAATGCAAAGCTAAAAGACAAGGTATCCC49480.15258978604432566No Hit
CGAGGTTCTCGCAAGCTTCAGGAATACAAAGCCAAAAGACAAGGTATCCC48040.1481490161998667No Hit
CGAGGTTCTCGCAAGCTTACCTATTTTTAGGATCAATCAATGTGAGGAAT47380.1461136633544897No Hit
CGAGGTTCTCGCAAGCTTCAGGAATACAAAGCTAAAAGACAAGGTGTCCC47200.14555856712393234No Hit
CGAGGTTCTCGCAAGCTTTCACCTCTTGTTAAAGCAGTAAATGGTCTTTG46860.1445100520217684No Hit
CGAGGTTCTCGCAAGCTTCAGGAACACAAAGCTAAAAGACAAGGTATCCC43080.13285303118006367No Hit
CGAGGTTCTCGCAAGCTTGTGGGGGTTATATCCTACTGCTCAAGGTCATC40680.1254517481059654No Hit
CGAGGTTCTCGCAAGCTTGTAATCTCAGCTACTCAGGAGGCTGAGGCAGG38290.11808130371134255No Hit
CGAGGTTCTCGCAAGCTTTGGTGGCCGAAATTCTCGTATGCCGTCTTCTG37030.11419563009744099TruSeq Adapter, Index 19 (95% over 24bp)
CGAGGTTCTCGCAAGCTTAGCGTTCCAATAACGGAACACTAGGCATAATG36660.11305459895685083No Hit
CGAGGTTCTCGCAAGCTTGTGGCCAAATGTAGACAAATTTTATGCATTAT36210.11166685838045741No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)