FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000709928

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000709928
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1641483
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGACCTTTAGTAAGCTTTCACTAGAGCAAAGGAAGTCCCTCAACATAA20962712.770586110242993No Hit
TCTGACCTTTAGTAAGCTTGCATGTCTGCCTTCTTCCTCATAGAAACACT233811.4243827075881992No Hit
TCTGACCTTTAGTAAGCTTTTTCAACCAGAGAAACAGTATGCTACAAGAG78790.4799927870102828No Hit
TCTGACCTTTAGTAAGCTTGCCCAGCACCAAGCAGTCATATAGGCCACAA64870.39519142141587815No Hit
TCTGACCTTTAGTAAGCTTGGGCTTGTAAAACTTGAGCTGCAAATTTTTA59170.3604667242974798No Hit
TCTGACCTTTAGTAAGCTTACTGTCCCATGAATTATCATAATATGACAAA56570.34462738876978927No Hit
TCTGACCTTTAGTAAGCTTTCTGGATTAAAAAATATATATATGCCACCAA55870.3403629522815649No Hit
TCTGACCTTTAGTAAGCTTCCAACTAATGAGAATGAATCTCATCAATGTC54570.33244328451771965No Hit
TCTGACCTTTAGTAAGCTTCCTGAAGATGATGTCTGGTTTGGTTTGTTGT53940.3286052916783177No Hit
TCTGACCTTTAGTAAGCTTCTGTTGAAGGCAATCCTACTGGAACTTTTAG50410.3071003476734148No Hit
TCTGACCTTTAGTAAGCTTGCCACTGCAGACTGGTGTTCAGAGAAAGCCA50130.30539457307812506No Hit
TCTGACCTTTAGTAAGCTTCCAATTGGAACTCAGAATACAAAGGAAATGG49310.3003990903347765No Hit
TCTGACCTTTAGTAAGCTTCCCTCTACAACATCCTCTCTTTCCAGCTGTG49240.2999726466859541No Hit
TCTGACCTTTAGTAAGCTTAGTCCTGAGACCCGGGGAGATTAAAAAAAAA48690.29662201801663496No Hit
TCTGACCTTTAGTAAGCTTAGTTTGGCTGGATATGAAATTCTGGGTTGAA46230.2816355697865893No Hit
TCTGACCTTTAGTAAGCTTATCCACCATGATCTAATTAAAAAATTTAAAG44620.2718273658636733No Hit
TCTGACCTTTAGTAAGCTTGATCTAATTAAAAAATTTAAAGAAGGATTAT43650.2659180753014195No Hit
TCTGACCTTTAGTAAGCTTTTATGCTCTGCTTCCCTTTTAAATATAAGTT41460.25257648114540326No Hit
TCTGACCTTTAGTAAGCTTCTATTATAAACATTGTGACTTTGTGCAATTT41380.2520891169753205No Hit
TCTGACCTTTAGTAAGCTTCTGTCCGAAGAGAGCAGTGTGCTCCTCCTCC40750.24825112413591854No Hit
TCTGACCTTTAGTAAGCTTGGTGGACCTCCAGAACCATTTGAGGGTACTT40610.24739823683827367No Hit
TCTGACCTTTAGTAAGCTTTTAAAGTATATTAGAGTTGATTGGGTTAGAC40190.2448395749453391No Hit
TCTGACCTTTAGTAAGCTTAGCAAGAATTTTAAATTACTGAGTAAATGAA37800.23027957036411587No Hit
TCTGACCTTTAGTAAGCTTCTGTGTTCCGAATTAGACACATCTTTATTTA36780.22406567719556036No Hit
TCTGACCTTTAGTAAGCTTACCTTAAGCCTAAGAGTTATATACAATTATT36750.2238829156317793No Hit
TCTGACCTTTAGTAAGCTTTGAAACATTAAGTATAGTCTTTATTCATGTT36640.2232127898979155No Hit
TCTGACCTTTAGTAAGCTTGGAATGGCGAAGAAGAGGAAATAGCAATTGC36210.22059320748372052No Hit
TCTGACCTTTAGTAAGCTTCCTTTTCAAGTCTCAAGTACATATTTAAAGC36190.2204713664411998No Hit
TCTGACCTTTAGTAAGCTTGAACAAAGCACAGTGGATATTGAAAGAAAGA36160.22028860487741878No Hit
TCTGACCTTTAGTAAGCTTCTTGCAGCACCTTTTTTTCACTGTCGCATCA35510.21632877099549613No Hit
TCTGACCTTTAGTAAGCTTCCTGAAGAGTCATCAGCAGCTGGAGACCATG34810.2120643345072718No Hit
TCTGACCTTTAGTAAGCTTAGGCAAATGATGAGACAATATTTCATCAAAA34560.2105413214757631No Hit
TCTGACCTTTAGTAAGCTTTTTCTATATTTTGGATACAAGTCCTTCGTCA34510.21023671886946133No Hit
TCTGACCTTTAGTAAGCTTGATTATGGGTGATGCTAGGCCCAATTTTCAC34330.20914014948677506No Hit
TCTGACCTTTAGTAAGCTTCAGGCCCTATACAACCTAGATTCATTCCTGC34170.20816542114660952No Hit
TCTGACCTTTAGTAAGCTTTTAGCCTTCAAGAAATGACATTTTGCTTTCA33010.2010986406804091No Hit
TCTGACCTTTAGTAAGCTTCACGGTGCAGTACATTTCCATATGGACAGGG32590.1985399787874745No Hit
TCTGACCTTTAGTAAGCTTTAATTCTCACAGAAGAACTGTGATAGTTTTT32580.19847905826621415No Hit
TCTGACCTTTAGTAAGCTTTAGGCCTGGGGCAAGGTCTGAGTTCCATGTG31780.1936054165653863No Hit
TCTGACCTTTAGTAAGCTTCCCGTTTTTCTAGGTGAGTGTCCCAAAGTTA31740.1933617344803449No Hit
TCTGACCTTTAGTAAGCTTCAAATGACGCTGATGCCCTGGTCTGCCACCC31140.18970650320472401No Hit
TCTGACCTTTAGTAAGCTTCTATGTAGCAACTAGTTCATTTCTCCATTCG30460.18556390775902035No Hit
TCTGACCTTTAGTAAGCTTCTAGGGAGCAGAGATGATGGTGTAATTCCTT30050.18306616638734607No Hit
TCTGACCTTTAGTAAGCTTTTCTGCTTCTTAGGGGGCTTTAATCTAAAGA29250.17819252468651822No Hit
TCTGACCTTTAGTAAGCTTTAAACCTTTTGGAATGTAAAGGCATATTTGT29240.17813160416525786No Hit
TCTGACCTTTAGTAAGCTTGAGGCCTGTCTTTTTTCCTTCTTCTTTTTTT28660.1745982139321577No Hit
TCTGACCTTTAGTAAGCTTTGGTCTGGAACACAGTTATCTGGAAATAGTT28130.17136942630535923No Hit
TCTGACCTTTAGTAAGCTTTCTAGTCTTTCTCAAGGAAACCTGTCCAAAG27810.1694199696250281No Hit
TCTGACCTTTAGTAAGCTTTCCTAGGGTGCTACACAGATATATAAAGCTG27730.16893260545494532No Hit
TCTGACCTTTAGTAAGCTTAATAATCAACATAGAAGTGTTATGAGCTTTA27620.1682624797210815No Hit
TCTGACCTTTAGTAAGCTTCATTATGTAGGCATGATTGATTAAATCATTA27040.1647290894879813No Hit
TCTGACCTTTAGTAAGCTTCAACTACGAATAAATAGCACCCATCTGCTTG26290.1601600503934552No Hit
TCTGACCTTTAGTAAGCTTACTACTGAAGTTTGAATAAAAACTATTTGTC26290.1601600503934552No Hit
TCTGACCTTTAGTAAGCTTTCATAGCCTTTAAAATGTCTCTATTCTGTAT26170.15942900413833103No Hit
TCTGACCTTTAGTAAGCTTTTAGTTGGAAAGATTGTTCAATGATCTAATT26020.1585151963194258No Hit
TCTGACCTTTAGTAAGCTTATTGCAGACATTTCCATCCATTTTAGAGTTC26020.1585151963194258No Hit
TCTGACCTTTAGTAAGCTTTCACAAGTACTAAAAATTTTTTTCTATCTTC25980.1582715142343844No Hit
TCTGACCTTTAGTAAGCTTCTCTGTTCCCTGATGCAGTGTGGATATTTTT25760.15693126276665673No Hit
TCTGACCTTTAGTAAGCTTAGTCATCTGACTTAGAAAGGACAGAAACTAT25710.15662666016035498No Hit
TCTGACCTTTAGTAAGCTTCATAAGTGAAGGAGAAATAAAATCCTTTACA24880.1515702568957461No Hit
TCTGACCTTTAGTAAGCTTAAGAGCAGTGGGTGCAATTATCCCAAATATC24590.14980356177919602No Hit
TCTGACCTTTAGTAAGCTTCAGGGCCTCTCACTTGCATAGGCTCTTTTAG24590.14980356177919602No Hit
TCTGACCTTTAGTAAGCTTTCAAACAGAATTTAAAGAGTTAACGCTGGCC24420.14876791291777008No Hit
TCTGACCTTTAGTAAGCTTTATTAAGTCATAGTTTCTCTTTCAATGGTAC24360.148402389790208No Hit
TCTGACCTTTAGTAAGCTTTAGTGTGCTCAGAATGACCAGAGAACCTTGT24230.1476104230138235No Hit
TCTGACCTTTAGTAAGCTTTGGTAACTGAGCTCCTCACTGCTCCAAGTTC24060.14657477415239756No Hit
TCTGACCTTTAGTAAGCTTCAAAGAAAGTTGGGGTTAGTTTTTTTGCCAA24030.1463920125886165No Hit
TCTGACCTTTAGTAAGCTTAACCTGTGCTTAGACATGCTTTTAAAAAGGT23890.14553912529097165No Hit
TCTGACCTTTAGTAAGCTTCAATATCTGAGGGCAGGAGAAGGTAGATATC23830.14517360216340955No Hit
TCTGACCTTTAGTAAGCTTAGATTTGATATTTGAGAAAATGGGAATCTTT23710.14444255590828536No Hit
TCTGACCTTTAGTAAGCTTTCAGGACCCCTCACTTACACTGACACCTTCT23680.14425979434450434No Hit
TCTGACCTTTAGTAAGCTTCATTTTCCTTGAGTGTAAAATGGTAATGATA23630.1439551917382026No Hit
TCTGACCTTTAGTAAGCTTATCCACCACGATCTAATTAAAAAATTTAAAG23380.1424321787066939No Hit
TCTGACCTTTAGTAAGCTTTCTATGACTCACCAAGGCTGGGTTGGGTGCT23360.1423103376641732No Hit
TCTGACCTTTAGTAAGCTTGCAAAATTTTCAGCCTGACCACGTGGTAGAA23300.14194481453661112No Hit
TCTGACCTTTAGTAAGCTTTAAAGATTGTTCTGTCCACTGTGTTGAGAAT23200.14133560932400763No Hit
TCTGACCTTTAGTAAGCTTCATCCTTACCCCTTTCAGTTGATGTCACAAA22860.1392643116011558No Hit
TCTGACCTTTAGTAAGCTTGCTTGAGGCTCATAAATCTTGCATTATTTGG22830.13908155003737474No Hit
TCTGACCTTTAGTAAGCTTTTCTTTCAAGGGTGAATAATATTAATACTTG22680.13816774221846953No Hit
TCTGACCTTTAGTAAGCTTCCTCCTTGGCCCACATTCCCTAACTTTGCAC22650.13798498065468848No Hit
TCTGACCTTTAGTAAGCTTATAAAAATTTAAATAGTAATACAAGACAGAA22550.13737577544208499No Hit
TCTGACCTTTAGTAAGCTTAAAGAATAAGTATATACCCAACACCTATTCC22410.1365228881444401No Hit
TCTGACCTTTAGTAAGCTTAGAAAAGAGAACCTAGGAATGAAGACACCAA22370.13627920605939872No Hit
TCTGACCTTTAGTAAGCTTTGATATTCTTGTGTTTTCTAGGATTTTCTAA22210.13530447771923315No Hit
TCTGACCTTTAGTAAGCTTATCAAGGAATCTCAACCCATGCAGTACTCTT22140.1348780340704107No Hit
TCTGACCTTTAGTAAGCTTTGGGATTCATTGAAGAGCTACACCCCAGAAA22110.13469527250662966No Hit
TCTGACCTTTAGTAAGCTTATCCCAGGCCCAGGTCTCCGAACTATTTGTC22070.13445159042158827No Hit
TCTGACCTTTAGTAAGCTTCCTAAATCATTAAAAGGATATCCTTTTAAAG21540.13122280279478982No Hit
TCTGACCTTTAGTAAGCTTACCCAGTGTGTTAGGCATAATACTAGCCCTC21370.1301871539333639No Hit
TCTGACCTTTAGTAAGCTTCTGCGTCAGCAATTCACAATCAATTTGCTTT21320.12988255132706217No Hit
TCTGACCTTTAGTAAGCTTATGGACATTATGCTCCTGCATAACATTGATT21320.12988255132706217No Hit
TCTGACCTTTAGTAAGCTTTACTCAGAGGCACACCCAAGAGAGAATAATC21200.12915150507193798No Hit
TCTGACCTTTAGTAAGCTTCTACCACCTCTTCTGGAATGGTTCTAGCTAG21180.1290296640294173No Hit
TCTGACCTTTAGTAAGCTTCAATTATAAAATGAATGACAGATGCACAAGA20880.12720204839160687No Hit
TCTGACCTTTAGTAAGCTTTCTCTTGATGTCCAATCACAGAATAACAGTA20810.12677560474278443No Hit
TCTGACCTTTAGTAAGCTTGAAACCTCTTGCCTGTTTGACTTGTCCACTA20790.1266537637002637No Hit
TCTGACCTTTAGTAAGCTTCTCGCACTAAACAGCCAAGTTGTGAATGCAA20780.12659284317900338No Hit
TCTGACCTTTAGTAAGCTTAAGGAGGGGAGATGAAGAGCAAGTGATTATG20740.126349161093962No Hit
TCTGACCTTTAGTAAGCTTTAAGGTGTGAAACCCAAGCATCTAAAATAGC20730.12628824057270163No Hit
TCTGACCTTTAGTAAGCTTTCTCCTCAAACTGGGAAGCAAGAGACCCCAG20600.12549627379631711No Hit
TCTGACCTTTAGTAAGCTTCATCTGTATTTAGCCACTCCCCATGGCTTGC20520.12500890962623432No Hit
TCTGACCTTTAGTAAGCTTAATCAGGCAGGACATTAGAGTCCAGGGGGGC20440.12452154545615154No Hit
TCTGACCTTTAGTAAGCTTATTTGAGCCTGTTGGGGTGAGGTGGGTGCAA20280.12354681711598597No Hit
TCTGACCTTTAGTAAGCTTCCAACTTACAGTTCAATATCCAGAGAAAATA20210.12312037346716354No Hit
TCTGACCTTTAGTAAGCTTGGTGGAGTTATTTAGCAGCCTGCTCACAGAT19920.12135367835061345No Hit
TCTGACCTTTAGTAAGCTTCTGGATAAATCTGACAGCAATGTCTTACATG19800.12062263209548926No Hit
TCTGACCTTTAGTAAGCTTTTTGTCATTTGTATTTTTAAGTATCACTATC19550.11909961906398055No Hit
TCTGACCTTTAGTAAGCTTTAGAATTGTAGTTCCAATGCTATCAAGGAAG19370.11800304968129428No Hit
TCTGACCTTTAGTAAGCTTGTTGTCATCAAATAAAACGCACAGCAAAGCT19140.11660187769230627No Hit
TCTGACCTTTAGTAAGCTTGTTAAAAATGCAGAATCCTAGACCTACCTCA19060.1161145135222235No Hit
TCTGACCTTTAGTAAGCTTTTATTTTTCTAAGTAAATGCCCAGGAGTACA18920.11526162622457863No Hit
TCTGACCTTTAGTAAGCTTCCATAAACTGAGCTTTTTGAAGGGGACCCGT18870.11495702361827688No Hit
TCTGACCTTTAGTAAGCTTAAGCTATCCCTCCTCCTTCCCTGCCGTCATC18790.11446965944819411No Hit
TCTGACCTTTAGTAAGCTTTTACTCATTGCAGAAGGCAAAGTGGGAGCAG18600.11331216954424748No Hit
TCTGACCTTTAGTAAGCTTCATTCTAAAGTTGATTGTCTTCAACCTCACC18400.11209375911904053No Hit
TCTGACCTTTAGTAAGCTTAGGTTTATAATCAGATAAATCCAATTCATTA18300.11148455390643705No Hit
TCTGACCTTTAGTAAGCTTCATAAAAGTATAAGCAATTCCAGATTCAAAA18280.11136271286391634No Hit
TCTGACCTTTAGTAAGCTTCCATAAAAACCTAAAATGACTGGGTTCAAGA17910.10910865357728347No Hit
TCTGACCTTTAGTAAGCTTCATTATACATATGCACGTGCATGCATGCTAA17870.10886497149224209No Hit
TCTGACCTTTAGTAAGCTTATGCTAACCATGCTAGAGAACAGGCTCATTT17770.1082557662796386No Hit
TCTGACCTTTAGTAAGCTTGGTGCTGGTGAATCAATGCTCTGTGGATTGA17610.10728103793947302No Hit
TCTGACCTTTAGTAAGCTTCATGAGGTCAGGACATATCTCCTTTTTGTTT17430.10618446855678676No Hit
TCTGACCTTTAGTAAGCTTCTGTTCTGAATTTTCCAGACCCATAATTTTG17400.1060017069930057No Hit
TCTGACCTTTAGTAAGCTTGAGAAAGTTTCTGCATTCCCTTGTTAATTTC17330.10557526334418327No Hit
TCTGACCTTTAGTAAGCTTAAATTTAAGACAATTTCTGATAAACCTGAAA17320.10551434282292294No Hit
TCTGACCTTTAGTAAGCTTACAGGCTTGCCTTCTGAATTTTTTCCACCTC17310.10545342230166257No Hit
TCTGACCTTTAGTAAGCTTTCCCATCAATTCCAGTTCCCAGAACAACAAC17280.10527066073788155No Hit
TCTGACCTTTAGTAAGCTTGAGTCTGGGGAGAAGGATGGTGGGAAAGAAA17270.10520974021662119No Hit
TCTGACCTTTAGTAAGCTTTACAATACCCTGATATATAGGAATTATACTA17070.10399132979141423No Hit
TCTGACCTTTAGTAAGCTTCCTCTAAGTATGAAATTTATGTATTTGGGCT16770.10216371415360379No Hit
TCTGACCTTTAGTAAGCTTCCCAGAGGTGTCACTGTTCTTCTCTTTGCTG16660.10149358841973996No Hit
TCTGACCTTTAGTAAGCTTCCTTGTGTTTCAAAGTATATCAACCCTAACA16620.10124990633469858No Hit
TCTGACCTTTAGTAAGCTTGGGTGCTAAAATCCCCTCCTGGAGTTGAGTT16580.10100622424965716No Hit
TCTGACCTTTAGTAAGCTTGTGTATGTGTCACGTAGTTCTCGTGCCAGGG16500.10051886007957439No Hit
TCTGACCTTTAGTAAGCTTGACATGAGAGGTTTCTCAGAGTTAGTAAACA16460.10027517799453299No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)