FastQCFastQC Report
Mon 12 Sep 2022
EGAF00000709933

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000709933
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5529224
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTGCGTGCAGATTCAAGCTTTTGAAATGGTTTCAGCTCTGTTCAATTG1342642.4282611809541446No Hit
AAGTGCGTGCAGATTCAAGCTTATTGAAGTTTAGGGAAAGAAGGTTGCTG783031.4161661744939253No Hit
AAGTGCGTGCAGATTCAAGCTTCAGTGTGTCCAGAGTTTTTATTGGGGCT546400.9882037696429011No Hit
AAGTGCGTGCAGATTCAAGCTTTGATCCTTGGTGATTGAGAGGATAGTGA334640.6052205517446934No Hit
AAGTGCGTGCAGATTCAAGCTTGCCAGAAATATATAAACACCTACCCCAG283060.5119344052619319No Hit
AAGTGCGTGCAGATTCAAGCTTTTCTGGAATATTATTTAAATTAAAATCT267070.4830153381378653No Hit
AAGTGCGTGCAGATTCAAGCTTGGATGAACGTCCGTGGTTGGCAGTACTC224670.40633188309968993No Hit
AAGTGCGTGCAGATTCAAGCTTAGGACAAAGGATTGACAATTGGTTGCAG187160.3384923453996438No Hit
AAGTGCGTGCAGATTCAAGCTTAGGGAACACTGTATGTGTAATTCCAATG184170.3330847149618102No Hit
AAGTGCGTGCAGATTCAAGCTTATTTTTGTTAACAAGCAGTCTGTGTGTG165030.2984686458714641No Hit
AAGTGCGTGCAGATTCAAGCTTAGGTGTCCAATTTGGGGCTGCCTGATTA163110.2949961875301127No Hit
AAGTGCGTGCAGATTCAAGCTTCATCCAATACCAAGTTTTAGCACAAGTC155330.28092549695942864No Hit
AAGTGCGTGCAGATTCAAGCTTCATAAGTGAAGGAGAAATAAAATACTTT152570.2759338380937361No Hit
AAGTGCGTGCAGATTCAAGCTTCACAGTCTGGGGAGGTGAAAGGGAAGCA146180.26437706267642624No Hit
AAGTGCGTGCAGATTCAAGCTTCTCTACTTTTACTTCAAGGCACTCTTAA144860.26198974756674714No Hit
AAGTGCGTGCAGATTCAAGCTTGAGGGGTGAGGAAAAAAAACCTAGAGGG138580.25063191507524385No Hit
AAGTGCGTGCAGATTCAAGCTTAAATCTTATCACTCATTTGGCACATAGC136000.24596579917905295No Hit
AAGTGCGTGCAGATTCAAGCTTGCGAGAACCTCGCACAGGGTTGGTATTT129590.2343728523206873No Hit
AAGTGCGTGCAGATTCAAGCTTAATAAATTATTAAAGAGCTATGTTACTC117890.21321255930307764No Hit
AAGTGCGTGCAGATTCAAGCTTCTAGTGCCTGATGCTCTATTATTAGTTT107590.1945842671593699No Hit
AAGTGCGTGCAGATTCAAGCTTTGTTATTGATTCCAAGTTTAATTCTGTT101740.18400412065056507No Hit
AAGTGCGTGCAGATTCAAGCTTATCCACCATGTCAATAAACGCATCTTGG101180.18299132030100426No Hit
AAGTGCGTGCAGATTCAAGCTTCCACTGCGAAAGCCATCACCACACACGA98010.17725814689366898No Hit
AAGTGCGTGCAGATTCAAGCTTGCAAAATACTTATTAAATTAAAAAACAG93240.16863125820187425No Hit
AAGTGCGTGCAGATTCAAGCTTCCTCTCCTTTCTTGGCCTTTGCCCACTT91410.16532157134527375No Hit
AAGTGCGTGCAGATTCAAGCTTATATAAATGTGAGGGGCACCAGTGCAGT89560.16197571304761754No Hit
AAGTGCGTGCAGATTCAAGCTTACAAGCAGAGAAGGAGCAGAGTGTCCGC89040.1610352555801682No Hit
AAGTGCGTGCAGATTCAAGCTTATGGAGGCCAGATATGGCAAAGTTGTGT85250.1541807675001049No Hit
AAGTGCGTGCAGATTCAAGCTTGCTCCCAAGGGACTGAAATCCTAGCACT85180.1540541674564098No Hit
AAGTGCGTGCAGATTCAAGCTTCTTCTGTTCTCTTTAACTCATCTACGTG83530.151070023569311No Hit
AAGTGCGTGCAGATTCAAGCTTCCCTTTTAGGGCAAAGCGTGGCGGATCA83220.15050936623294697No Hit
AAGTGCGTGCAGATTCAAGCTTCCTCAGATCACTGAGATAGGAATGAAAA83190.15045510907136336No Hit
AAGTGCGTGCAGATTCAAGCTTTTTAAAGTAAACTAAATACTTAAATTAT83120.15032850902766826No Hit
AAGTGCGTGCAGATTCAAGCTTAACTAAATTAGGCTTATAGGAAAATGAT80370.1453549358825036No Hit
AAGTGCGTGCAGATTCAAGCTTAGTCCTCCGTTATACTAGTGGGAACAAT79680.14410702116608046No Hit
AAGTGCGTGCAGATTCAAGCTTTAATATTTAATACTGCTATCTTTTAGTA78390.14177396321798502No Hit
AAGTGCGTGCAGATTCAAGCTTATCCACCAAGATCAAGTTGGCTTCATCC72190.13056081649070467No Hit
AAGTGCGTGCAGATTCAAGCTTTCCTTACCTAGGTTCACAGCCACTGACA71260.1288788444816126No Hit
AAGTGCGTGCAGATTCAAGCTTTTCTTCTAAATTAGGCTATTCGAAAAAA71130.12864373011475028No Hit
AAGTGCGTGCAGATTCAAGCTTTTTTTGCCTGTAATGTTTTATTTTAAAA70850.12813732993996987No Hit
AAGTGCGTGCAGATTCAAGCTTAGGGTATAGGGACGCTAGTTCAGACTAA70630.12773944408835672No Hit
AAGTGCGTGCAGATTCAAGCTTTACAGAACCATCTCCACCCCCACTCCTA68800.12442975723175621No Hit
AAGTGCGTGCAGATTCAAGCTTGAAAGCAAGGCCTGTACTCCACTTGAAA67840.12269352806108054No Hit
AAGTGCGTGCAGATTCAAGCTTGAAGTTGGTGAGCAGAAATACAATTTAA67470.1220243564015493No Hit
AAGTGCGTGCAGATTCAAGCTTGTGCCACCATGTCAATAAACGCATCTTG66510.12028812723087363No Hit
AAGTGCGTGCAGATTCAAGCTTCTTGGATACCCTCTACACCATCCCAAGT66480.12023387006929No Hit
AAGTGCGTGCAGATTCAAGCTTGGCCTATATGTTGAGTTTTATTCCTCCC64960.11748484054905353No Hit
AAGTGCGTGCAGATTCAAGCTTGGTCTTCGGAAGCTCGTATGCCGTCTTC64340.11636352587632551No Hit
AAGTGCGTGCAGATTCAAGCTTCATGTCAATAAACGCATCTTGGTGTCTG64310.1163092687147419No Hit
AAGTGCGTGCAGATTCAAGCTTTGAGAGTTGTTGGAAGAGAGCCTAGTGG64140.11600181146576807No Hit
AAGTGCGTGCAGATTCAAGCTTTCCATTAGTTTTAATAGGGGATTTTGTA62400.11285489609391841No Hit
AAGTGCGTGCAGATTCAAGCTTCAATGTCTCCATTCCTTCACTGCTAAAA62180.11245701024230526No Hit
AAGTGCGTGCAGATTCAAGCTTGGTCAATATGGTATGATAGGATCCCATT62140.11238466736019376No Hit
AAGTGCGTGCAGATTCAAGCTTTCAGGGCTTCCACAAACAATTTTGTTTT61370.11099206687954766No Hit
AAGTGCGTGCAGATTCAAGCTTGGTCACAGCATATAACAAGTGTCTAAAC60950.11023246661737704No Hit
AAGTGCGTGCAGATTCAAGCTTCCATTTCTTTCATTTTTACTAAGACAAC60550.10950903779626218No Hit
AAGTGCGTGCAGATTCAAGCTTAATGATAATCTAAAGGATGATCTGGTCC59680.10793558011033737No Hit
AAGTGCGTGCAGATTCAAGCTTCTACAGTTACCTTTATTTTGCATGTCAA59430.10748343709714057No Hit
AAGTGCGTGCAGATTCAAGCTTAACAGTTTGCAGAGTGTGCACGTTTTAA59290.10723023700975037No Hit
AAGTGCGTGCAGATTCAAGCTTCTAGAATAATTTATATGTGATAGAGGAT57600.10417375024054007No Hit
AAGTGCGTGCAGATTCAAGCTTCATAAGTGAAGGAGAAATAAAATCCTTT57010.10310669272939567No Hit
AAGTGCGTGCAGATTCAAGCTTCTATGTGGATCCCAGCTAGAGCTCATAC56620.10240134962880866No Hit
AAGTGCGTGCAGATTCAAGCTTTTTAATTGCTTCTGGGAAAAGGACATAG56580.10232900674669719No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)