FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000709936

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000709936
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1811979
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGACCTTTAGTAAGCTTTCACTAGAGCAAAGGAAGTCCCTCAACATAA22822112.595123894923727No Hit
TCTGACCTTTAGTAAGCTTGCATGTCTGCCTTCTTCCTCATAGAAACACT265311.4642001921655825No Hit
TCTGACCTTTAGTAAGCTTTTTCAACCAGAGAAACAGTATGCTACAAGAG89750.49531479117583593No Hit
TCTGACCTTTAGTAAGCTTGCCCAGCACCAAGCAGTCATATAGGCCACAA74220.4096073961122066No Hit
TCTGACCTTTAGTAAGCTTGGGCTTGTAAAACTTGAGCTGCAAATTTTTA64640.3567370262017385No Hit
TCTGACCTTTAGTAAGCTTTCTGGATTAAAAAATATATATATGCCACCAA63450.3501696211711063No Hit
TCTGACCTTTAGTAAGCTTACTGTCCCATGAATTATCATAATATGACAAA61590.33990460154339536No Hit
TCTGACCTTTAGTAAGCTTCCAACTAATGAGAATGAATCTCATCAATGTC61580.339849413265827No Hit
TCTGACCTTTAGTAAGCTTCCTGAAGATGATGTCTGGTTTGGTTTGTTGT61380.33874564771446025No Hit
TCTGACCTTTAGTAAGCTTGCCACTGCAGACTGGTGTTCAGAGAAAGCCA58060.320423139561772No Hit
TCTGACCTTTAGTAAGCTTCTGTTGAAGGCAATCCTACTGGAACTTTTAG57870.3193745622879735No Hit
TCTGACCTTTAGTAAGCTTAGTCCTGAGACCCGGGGAGATTAAAAAAAAA55290.30513598667534225No Hit
TCTGACCTTTAGTAAGCTTCCCTCTACAACATCCTCTCTTTCCAGCTGTG54520.3008864893025802No Hit
TCTGACCTTTAGTAAGCTTCCAATTGGAACTCAGAATACAAAGGAAATGG54070.298403016812005No Hit
TCTGACCTTTAGTAAGCTTGATCTAATTAAAAAATTTAAAGAAGGATTAT50350.2778729775565832No Hit
TCTGACCTTTAGTAAGCTTAGTTTGGCTGGATATGAAATTCTGGGTTGAA49920.27549988162114464No Hit
TCTGACCTTTAGTAAGCTTATCCACCATGATCTAATTAAAAAATTTAAAG48990.27036737180728915No Hit
TCTGACCTTTAGTAAGCTTGGTGGACCTCCAGAACCATTTGAGGGTACTT46350.25579766652924785No Hit
TCTGACCTTTAGTAAGCTTTTATGCTCTGCTTCCCTTTTAAATATAAGTT46210.25502503064329113No Hit
TCTGACCTTTAGTAAGCTTTTAAAGTATATTAGAGTTGATTGGGTTAGAC45610.2517137339891908No Hit
TCTGACCTTTAGTAAGCTTCTATTATAAACATTGTGACTTTGTGCAATTT45540.25132741604621245No Hit
TCTGACCTTTAGTAAGCTTCTGTCCGAAGAGAGCAGTGTGCTCCTCCTCC44800.2472434835061554No Hit
TCTGACCTTTAGTAAGCTTCTGTGTTCCGAATTAGACACATCTTTATTTA42120.23245302511784074No Hit
TCTGACCTTTAGTAAGCTTAGCAAGAATTTTAAATTACTGAGTAAATGAA41290.2278723980796687No Hit
TCTGACCTTTAGTAAGCTTGAACAAAGCACAGTGGATATTGAAAGAAAGA40970.22610637319748186No Hit
TCTGACCTTTAGTAAGCTTCTTGCAGCACCTTTTTTTCACTGTCGCATCA40200.22185687582471983No Hit
TCTGACCTTTAGTAAGCTTGGAATGGCGAAGAAGAGGAAATAGCAATTGC40070.22113942821633142No Hit
TCTGACCTTTAGTAAGCTTTTTCTATATTTTGGATACAAGTCCTTCGTCA39620.2186559557257562No Hit
TCTGACCTTTAGTAAGCTTGATTATGGGTGATGCTAGGCCCAATTTTCAC39520.2181040729500728No Hit
TCTGACCTTTAGTAAGCTTTGAAACATTAAGTATAGTCTTTATTCATGTT39360.2172210605089794No Hit
TCTGACCTTTAGTAAGCTTCCTGAAGAGTCATCAGCAGCTGGAGACCATG39340.21711068395384275No Hit
TCTGACCTTTAGTAAGCTTACCTTAAGCCTAAGAGTTATATACAATTATT39280.2167795542884327No Hit
TCTGACCTTTAGTAAGCTTCAGGCCCTATACAACCTAGATTCATTCCTGC38920.21479277629597254No Hit
TCTGACCTTTAGTAAGCTTAGGCAAATGATGAGACAATATTTCATCAAAA38400.21192298586241892No Hit
TCTGACCTTTAGTAAGCTTCCTTTTCAAGTCTCAAGTACATATTTAAAGC38250.21109516169889384No Hit
TCTGACCTTTAGTAAGCTTTTAGCCTTCAAGAAATGACATTTTGCTTTCA37410.20645934638315344No Hit
TCTGACCTTTAGTAAGCTTCCCGTTTTTCTAGGTGAGTGTCCCAAAGTTA36770.2029272966187798No Hit
TCTGACCTTTAGTAAGCTTCAAATGACGCTGATGCCCTGGTCTGCCACCC35400.19536650259191746No Hit
TCTGACCTTTAGTAAGCTTTAATTCTCACAGAAGAACTGTGATAGTTTTT35050.1934349128770256No Hit
TCTGACCTTTAGTAAGCTTTAGGCCTGGGGCAAGGTCTGAGTTCCATGTG34960.19293821837891056No Hit
TCTGACCTTTAGTAAGCTTCTATGTAGCAACTAGTTCATTTCTCCATTCG34490.19034436933319868No Hit
TCTGACCTTTAGTAAGCTTCACGGTGCAGTACATTTCCATATGGACAGGG34390.1897924865575153No Hit
TCTGACCTTTAGTAAGCTTCTAGGGAGCAGAGATGATGGTGTAATTCCTT33740.1862052485155733No Hit
TCTGACCTTTAGTAAGCTTTAAACCTTTTGGAATGTAAAGGCATATTTGT33380.18421847052311313No Hit
TCTGACCTTTAGTAAGCTTGAGGCCTGTCTTTTTTCCTTCTTCTTTTTTT32760.18079679731387616No Hit
TCTGACCTTTAGTAAGCTTTTCTGCTTCTTAGGGGGCTTTAATCTAAAGA31800.17549872266731567No Hit
TCTGACCTTTAGTAAGCTTTGGTCTGGAACACAGTTATCTGGAAATAGTT31750.175222781279474No Hit
TCTGACCTTTAGTAAGCTTTCCTAGGGTGCTACACAGATATATAAAGCTG31220.17229780256835206No Hit
TCTGACCTTTAGTAAGCTTAATAATCAACATAGAAGTGTTATGAGCTTTA31190.17213223773564704No Hit
TCTGACCTTTAGTAAGCTTTTAGTTGGAAAGATTGTTCAATGATCTAATT30670.16926244730209344No Hit
TCTGACCTTTAGTAAGCTTCAACTACGAATAAATAGCACCCATCTGCTTG30300.16722048103206494No Hit
TCTGACCTTTAGTAAGCTTTCTAGTCTTTCTCAAGGAAACCTGTCCAAAG30160.16644784514610822No Hit
TCTGACCTTTAGTAAGCTTCATTATGTAGGCATGATTGATTAAATCATTA30140.16633746859097154No Hit
TCTGACCTTTAGTAAGCTTTCACAAGTACTAAAAATTTTTTTCTATCTTC30050.16584077409285647No Hit
TCTGACCTTTAGTAAGCTTACTACTGAAGTTTGAATAAAAACTATTTGTC29900.1650129499293314No Hit
TCTGACCTTTAGTAAGCTTCTCTGTTCCCTGATGCAGTGTGGATATTTTT29620.16346767815741794No Hit
TCTGACCTTTAGTAAGCTTTAGTGTGCTCAGAATGACCAGAGAACCTTGT28760.15872148628654084No Hit
TCTGACCTTTAGTAAGCTTATTGCAGACATTTCCATCCATTTTAGAGTTC28690.15833516834356248No Hit
TCTGACCTTTAGTAAGCTTCATAAGTGAAGGAGAAATAAAATCCTTTACA28320.15629320207353395No Hit
TCTGACCTTTAGTAAGCTTAGTCATCTGACTTAGAAAGGACAGAAACTAT28310.15623801379596564No Hit
TCTGACCTTTAGTAAGCTTAAGAGCAGTGGGTGCAATTATCCCAAATATC28290.15612763724082895No Hit
TCTGACCTTTAGTAAGCTTTCATAGCCTTTAAAATGTCTCTATTCTGTAT28230.15579650757541894No Hit
TCTGACCTTTAGTAAGCTTTATTAAGTCATAGTTTCTCTTTCAATGGTAC27870.15380972958295874No Hit
TCTGACCTTTAGTAAGCTTTCAAACAGAATTTAAAGAGTTAACGCTGGCC27200.15011211498588006No Hit
TCTGACCTTTAGTAAGCTTCAAAGAAAGTTGGGGTTAGTTTTTTTGCCAA27180.15000173843074338No Hit
TCTGACCTTTAGTAAGCTTCAGGGCCTCTCACTTGCATAGGCTCTTTTAG27000.1490083494345133No Hit
TCTGACCTTTAGTAAGCTTAACCTGTGCTTAGACATGCTTTTAAAAAGGT26940.14867721976910328No Hit
TCTGACCTTTAGTAAGCTTTGGTAACTGAGCTCCTCACTGCTCCAAGTTC26690.14729751282989484No Hit
TCTGACCTTTAGTAAGCTTAGATTTGATATTTGAGAAAATGGGAATCTTT26680.1472423245523265No Hit
TCTGACCTTTAGTAAGCTTTCAGGACCCCTCACTTACACTGACACCTTCT26300.14514517000472962No Hit
TCTGACCTTTAGTAAGCTTATCCACCACGATCTAATTAAAAAATTTAAAG26290.14508998172716128No Hit
TCTGACCTTTAGTAAGCTTTAAAGATTGTTCTGTCCACTGTGTTGAGAAT26190.14453809895147793No Hit
TCTGACCTTTAGTAAGCTTCATTTTCCTTGAGTGTAAAATGGTAATGATA26120.14415178100849954No Hit
TCTGACCTTTAGTAAGCTTTCTATGACTCACCAAGGCTGGGTTGGGTGCT25880.1428272623468594No Hit
TCTGACCTTTAGTAAGCTTCAATATCTGAGGGCAGGAGAAGGTAGATATC25820.1424961326814494No Hit
TCTGACCTTTAGTAAGCTTGCAAAATTTTCAGCCTGACCACGTGGTAGAA25470.14056454296655754No Hit
TCTGACCTTTAGTAAGCTTTGATATTCTTGTGTTTTCTAGGATTTTCTAA25300.1396263422478958No Hit
TCTGACCTTTAGTAAGCTTCATCCTTACCCCTTTCAGTTGATGTCACAAA25190.1390192711946441No Hit
TCTGACCTTTAGTAAGCTTGCTTGAGGCTCATAAATCTTGCATTATTTGG25090.1384673884189607No Hit
TCTGACCTTTAGTAAGCTTATCAAGGAATCTCAACCCATGCAGTACTCTT24970.13780512908814066No Hit
TCTGACCTTTAGTAAGCTTTGGGATTCATTGAAGAGCTACACCCCAGAAA24910.13747399942273061No Hit
TCTGACCTTTAGTAAGCTTATAAAAATTTAAATAGTAATACAAGACAGAA24830.1370324932021839No Hit
TCTGACCTTTAGTAAGCTTTTCTTTCAAGGGTGAATAATATTAATACTTG24660.13609429248352217No Hit
TCTGACCTTTAGTAAGCTTAAAGAATAAGTATATACCCAACACCTATTCC24580.13565278626297544No Hit
TCTGACCTTTAGTAAGCTTCTACCACCTCTTCTGGAATGGTTCTAGCTAG24530.13537684487513377No Hit
TCTGACCTTTAGTAAGCTTATGGACATTATGCTCCTGCATAACATTGATT24360.134438644156472No Hit
TCTGACCTTTAGTAAGCTTCCTAAATCATTAAAAGGATATCCTTTTAAAG24110.13305893721726356No Hit
TCTGACCTTTAGTAAGCTTCTGCGTCAGCAATTCACAATCAATTTGCTTT23930.13206554822103345No Hit
TCTGACCTTTAGTAAGCTTATCCCAGGCCCAGGTCTCCGAACTATTTGTC23610.13029952333884665No Hit
TCTGACCTTTAGTAAGCTTCCTCCTTGGCCCACATTCCCTAACTTTGCAC23600.1302443350612783No Hit
TCTGACCTTTAGTAAGCTTGTTGTCATCAAATAAAACGCACAGCAAAGCT23530.12985801711829995No Hit
TCTGACCTTTAGTAAGCTTACCCAGTGTGTTAGGCATAATACTAGCCCTC23430.12930613434261656No Hit
TCTGACCTTTAGTAAGCTTCTCGCACTAAACAGCCAAGTTGTGAATGCAA23360.12891981639963818No Hit
TCTGACCTTTAGTAAGCTTAGAAAAGAGAACCTAGGAATGAAGACACCAA23290.12853349845665982No Hit
TCTGACCTTTAGTAAGCTTCTGGATAAATCTGACAGCAATGTCTTACATG23220.12814718051368146No Hit
TCTGACCTTTAGTAAGCTTTACTCAGAGGCACACCCAAGAGAGAATAATC23150.12776086257070307No Hit
TCTGACCTTTAGTAAGCTTTTTGTCATTTGTATTTTTAAGTATCACTATC22920.1264915321866313No Hit
TCTGACCTTTAGTAAGCTTTCTCCTCAAACTGGGAAGCAAGAGACCCCAG22840.12605002596608458No Hit
TCTGACCTTTAGTAAGCTTTAAGGTGTGAAACCCAAGCATCTAAAATAGC22420.12373211830821439No Hit
TCTGACCTTTAGTAAGCTTCCAACTTACAGTTCAATATCCAGAGAAAATA22350.12334580036523603No Hit
TCTGACCTTTAGTAAGCTTCATCTGTATTTAGCCACTCCCCATGGCTTGC22330.12323542381009936No Hit
TCTGACCTTTAGTAAGCTTGGTGGAGTTATTTAGCAGCCTGCTCACAGAT22290.123014670699826No Hit
TCTGACCTTTAGTAAGCTTCAATTATAAAATGAATGACAGATGCACAAGA22240.1227387293119843No Hit
TCTGACCTTTAGTAAGCTTAAGGAGGGGAGATGAAGAGCAAGTGATTATG22240.1227387293119843No Hit
TCTGACCTTTAGTAAGCTTCCATAAACTGAGCTTTTTGAAGGGGACCCGT22180.12240759964657426No Hit
TCTGACCTTTAGTAAGCTTATTTGAGCCTGTTGGGGTGAGGTGGGTGCAA22060.12174534031575421No Hit
TCTGACCTTTAGTAAGCTTGAAACCTCTTGCCTGTTTGACTTGTCCACTA21810.12036563337654575No Hit
TCTGACCTTTAGTAAGCTTTCTCTTGATGTCCAATCACAGAATAACAGTA21790.12025525682140907No Hit
TCTGACCTTTAGTAAGCTTCCATAAAAACCTAAAATGACTGGGTTCAAGA21500.11865479677192727No Hit
TCTGACCTTTAGTAAGCTTAATCAGGCAGGACATTAGAGTCCAGGGGGGC21270.11738546638785548No Hit
TCTGACCTTTAGTAAGCTTCATAAAAGTATAAGCAATTCCAGATTCAAAA20930.11550906495053198No Hit
TCTGACCTTTAGTAAGCTTCATTCTAAAGTTGATTGTCTTCAACCTCACC20760.11457086423187024No Hit
TCTGACCTTTAGTAAGCTTTTACTCATTGCAGAAGGCAAAGTGGGAGCAG20360.11236333312913671No Hit
TCTGACCTTTAGTAAGCTTCATTATACATATGCACGTGCATGCATGCTAA20310.11208739174129502No Hit
TCTGACCTTTAGTAAGCTTGAGTCTGGGGAGAAGGATGGTGGGAAAGAAA20310.11208739174129502No Hit
TCTGACCTTTAGTAAGCTTGTTAAAAATGCAGAATCCTAGACCTACCTCA20170.11131475585533829No Hit
TCTGACCTTTAGTAAGCTTTTATTTTTCTAAGTAAATGCCCAGGAGTACA19820.10938316614044644No Hit
TCTGACCTTTAGTAAGCTTAGGTTTATAATCAGATAAATCCAATTCATTA19810.1093279778628781No Hit
TCTGACCTTTAGTAAGCTTTAGAATTGTAGTTCCAATGCTATCAAGGAAG19760.1090520364750364No Hit
TCTGACCTTTAGTAAGCTTGGTGCTGGTGAATCAATGCTCTGTGGATTGA19700.10872090680962639No Hit
TCTGACCTTTAGTAAGCTTAAGCTATCCCTCCTCCTTCCCTGCCGTCATC19440.10728601159284959No Hit
TCTGACCTTTAGTAAGCTTGACATGAGAGGTTTCTCAGAGTTAGTAAACA19380.10695488192743956No Hit
TCTGACCTTTAGTAAGCTTATGCTAACCATGCTAGAGAACAGGCTCATTT19340.1067341288171662No Hit
TCTGACCTTTAGTAAGCTTTCCAGGTTCCACCTCTGGATGCTAATCACCA19310.10656856398446118No Hit
TCTGACCTTTAGTAAGCTTCATGAGGTCAGGACATATCTCCTTTTTGTTT19110.10546479843309442No Hit
TCTGACCTTTAGTAAGCTTACAGGCTTGCCTTCTGAATTTTTTCCACCTC18960.10463697426956935No Hit
TCTGACCTTTAGTAAGCTTCTGTTCTGAATTTTCCAGACCCATAATTTTG18940.10452659771443266No Hit
TCTGACCTTTAGTAAGCTTGTGTATGTGTCACGTAGTTCTCGTGCCAGGG18790.1036987735509076No Hit
TCTGACCTTTAGTAAGCTTTACAATACCCTGATATATAGGAATTATACTA18750.10347802044063424No Hit
TCTGACCTTTAGTAAGCTTGAGAAAGTTTCTGCATTCCCTTGTTAATTTC18740.1034228321630659No Hit
TCTGACCTTTAGTAAGCTTCAGTAGCCAATTCGATAAAGTGGAAGAAAGG18720.10331245560792923No Hit
TCTGACCTTTAGTAAGCTTCCTCTAAGTATGAAATTTATGTATTTGGGCT18640.10287094938738253No Hit
TCTGACCTTTAGTAAGCTTGGGTGCTAAAATCCCCTCCTGGAGTTGAGTT18640.10287094938738253No Hit
TCTGACCTTTAGTAAGCTTAAATTTAAGACAATTTCTGATAAACCTGAAA18620.10276057283224585No Hit
TCTGACCTTTAGTAAGCTTTATGAAAAAGAGGCCAGAGGAGGGAGACTGG18280.10088417139492234No Hit
TCTGACCTTTAGTAAGCTTCCTTGTGTTTCAAAGTATATCAACCCTAACA18230.10060823000708065No Hit
TCTGACCTTTAGTAAGCTTTCCCATCAATTCCAGTTCCCAGAACAACAAC18200.10044266517437565No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)