FastQCFastQC Report
Tue 13 Sep 2022
EGAF00000709937

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000709937
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6058300
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACAGCCACACCAGCAAGCTTATCTCAGATGATTAAATGGCAGCAGATA69160211.415776703035505No Hit
GAACAGCCACACCAGCAAGCTTGATACGGCGCCCAGCAAAATGTCGTATG1112081.8356304573890365No Hit
GAACAGCCACACCAGCAAGCTTAATGATACGGCGCCCAGCAAAATGTCGT826091.36356733737187No Hit
GAACAGCCACACCAGCAAGCTTTGTCATTTTGCTCTTAACAATAAACCTT806701.33156165921133No Hit
GAACAGCCACACCAGCAAGCTTGCTTTGAACTCCAAGTACCTCCAAGGTC791621.3066701880065363No Hit
GAACAGCCACACCAGCAAGCTTATGATACGGCGCCCAGCAAAATGTCGTA715781.181486555634419No Hit
GAACAGCCACACCAGCAAGCTTAGCTCAGTTAACTCAGCACACAGTTCGT701401.1577505240744104No Hit
GAACAGCCACACCAGCAAGCTTGAGACGGCGCCCAGCAAAATGTCGTATG599520.9895845369162967No Hit
GAACAGCCACACCAGCAAGCTTCGCTTCTGTGAGCCCCCTTGTCTGTTGG527640.8709373916775333No Hit
GAACAGCCACACCAGCAAGCTTCTCCCTGGGCAGGCTGTGTTGCTAACAC486340.8027664526352276No Hit
GAACAGCCACACCAGCAAGCTTATGAGACGGCGCCCAGCAAAATGTCGTA443990.7328623541257449No Hit
GAACAGCCACACCAGCAAGCTTAATGAGACGGCGCCCAGCAAAATGTCGT401170.6621824604261922No Hit
GAACAGCCACACCAGCAAGCTTATACGGCGCCCAGCAAAATGTCGTATGC400070.660366769555816No Hit
GAACAGCCACACCAGCAAGCTTACTGAATCAAGATCTTACTTGGTGGGGT278220.4592377399600548No Hit
GAACAGCCACACCAGCAAGCTTATGCTTAAAACTATTTAAATTCAAAAGT269390.444662694155126No Hit
GAACAGCCACACCAGCAAGCTTAGACGGCGCCCAGCAAAATGTCGTATGC266100.4392321278246372No Hit
GAACAGCCACACCAGCAAGCTTCCATGTGATCTGCCTGCCTCAGCCTCCC255860.422329696449499No Hit
GAACAGCCACACCAGCAAGCTTACGGCGCCCAGCAAAATGTCGTATGCCG237810.3925358598946899No Hit
GAACAGCCACACCAGCAAGCTTCTTGGCCCCAGGACTGCCCCAGGGGTGG203540.33596883614215206No Hit
GAACAGCCACACCAGCAAGCTTATGAAGTGTTACAAGATAAGGGCAACAG195780.3231599623656801No Hit
GAACAGCCACACCAGCAAGCTTCAACTACATCCTAATTACCCAGTGGGAA189110.31215027317894456No Hit
GAACAGCCACACCAGCAAGCTTTGCCTGGCTGACAAAGGGAGGAGGGGCA182690.30155324100820363No Hit
GAACAGCCACACCAGCAAGCTTGGCCAGCCTTGGCTGTTTTCTGATCCCG177360.29275539342719903No Hit
GAACAGCCACACCAGCAAGCTTCATGTGATCTGCCTGCCTCAGCCTCCCA171120.2824554743079742No Hit
GAACAGCCACACCAGCAAGCTTCCCAGAATGGTTGCTGTCTCTGAAGGAT170110.28078833996335606No Hit
GAACAGCCACACCAGCAAGCTTTTCTTTAGTAAGACTAATGCTATCAAGG167570.2765957446808511No Hit
GAACAGCCACACCAGCAAGCTTATGACATAAAGGCTTTATTTATTTATTT159720.26363831437862106No Hit
GAACAGCCACACCAGCAAGCTTCGTGTCCATTTACAGTCACTTTCCATTG140040.23115395407952727No Hit
GAACAGCCACACCAGCAAGCTTTAGAGCTGAGCCTTGGAGGAGGGGTGTC134330.2217288678342109No Hit
GAACAGCCACACCAGCAAGCTTCCCAGTCCCTGGATTGGATTCCCACATT129190.21324463958536222No Hit
GAACAGCCACACCAGCAAGCTTATCATAGCATCTTAAAGTTGGAAGGGAC127340.2101909776670023No Hit
GAACAGCCACACCAGCAAGCTTTTACCTGGTGAATTTAAGAAGACATAAT127120.20982783949292705No Hit
GAACAGCCACACCAGCAAGCTTGATCAGGAGAATATTGAAGGGAGACTCG124880.20613043262961558No Hit
GAACAGCCACACCAGCAAGCTTACAAGTTCGAGACCAGCCTGGGTAACAT121720.200914447947444No Hit
GAACAGCCACACCAGCAAGCTTCATAAGTGAAGGAGAAATAAAATACTTT118680.19589653863294984No Hit
GAACAGCCACACCAGCAAGCTTTCAAAAGGAGTACAAGTTTCATAGAGCA116250.19188551243748245No Hit
GAACAGCCACACCAGCAAGCTTTACGGCGCCCAGCAAAATGTCGTATGCC114250.18858425630952577No Hit
GAACAGCCACACCAGCAAGCTTAAGCAATGTGGCCTCCCAAAATGCTGGG114080.18830364953864945No Hit
GAACAGCCACACCAGCAAGCTTGACCACTTCTAGCCAGGGCCTCGGTGGC112260.18529950646220888No Hit
GAACAGCCACACCAGCAAGCTTCAGTCTCCCAAAGTGCTGGGATTACAGG110260.18199825033425218No Hit
GAACAGCCACACCAGCAAGCTTTGATACGGCGCCCAGCAAAATGTCGTAT109580.18087582325074691No Hit
GAACAGCCACACCAGCAAGCTTTTTTGGGGGATGATGGAAATTTATTTAA109550.18082630440882758No Hit
GAACAGCCACACCAGCAAGCTTACTGAATCAAGATCTTGCTTGGTGGGGT108360.17886205701269334No Hit
GAACAGCCACACCAGCAAGCTTCAGTGGCCTGCTCCATCCCCTAATGACT104900.17315088391132827No Hit
GAACAGCCACACCAGCAAGCTTCGCTTCTATAATGATGATGAGCTAGTCT103200.17034481620256506No Hit
GAACAGCCACACCAGCAAGCTTCTACCTAAGGTCAACATTATTCTCCCAT103120.1702127659574468No Hit
GAACAGCCACACCAGCAAGCTTAAAATCAAAGGGGATGAGTTGGGTCTTT101060.1668124721456514No Hit
GAACAGCCACACCAGCAAGCTTCTTCAGGATAGGGAGGGGCCCCTGTCAC100170.1653434131687107No Hit
GAACAGCCACACCAGCAAGCTTGGCTGCGTGCCTTTCTCCAGGTTTCCCT99270.16385784791113017No Hit
GAACAGCCACACCAGCAAGCTTTGTTCTCTTCATCTGGCCTCAGAGTTAG98470.1625373454599475No Hit
GAACAGCCACACCAGCAAGCTTGTTGACACCGGGGTAACATCCACCCAGG97000.16011092220589934No Hit
GAACAGCCACACCAGCAAGCTTGAGAAGGGAGGCTGGTCTTGGGAGAGGC95700.1579651057227275No Hit
GAACAGCCACACCAGCAAGCTTTGTGCTTGGGTTTGGGCACTGCAGCTGT93340.1540696234917386No Hit
GAACAGCCACACCAGCAAGCTTCGTCCAGGGTTCTTTATTTTTGTCTGCT91410.1508839113282604No Hit
GAACAGCCACACCAGCAAGCTTCCCTTCACAGCCCTGCTGCAACCTCTCA88310.14576696432992753No Hit
GAACAGCCACACCAGCAAGCTTGGCTGGTGATCAAATTGCCTGGGGAGCT88100.14542033243649208No Hit
GAACAGCCACACCAGCAAGCTTGGGAACCACTAGCTTTGGAGCTTCAAAG87790.1449086377366588No Hit
GAACAGCCACACCAGCAAGCTTGGAGGCTGAAGCAGCTCCACTGTGAATG87560.14452899328194377No Hit
GAACAGCCACACCAGCAAGCTTTGGGTATATGACAGGAGGCAGCGTTTGA86310.14246570820197085No Hit
GAACAGCCACACCAGCAAGCTTGGCACTATTCCAGACCCCGTGGGGCACT85970.14190449466021823No Hit
GAACAGCCACACCAGCAAGCTTATCCACCATGTGATCTGCCTGCCTCAGC85610.141310268557186No Hit
GAACAGCCACACCAGCAAGCTTGCATTCTAGTCCGAGGAGCAGACAGAAT85310.14081508013799252No Hit
GAACAGCCACACCAGCAAGCTTCTAGAAAATTCCAACTAAGAAACTGGAG84310.13916445207401415No Hit
GAACAGCCACACCAGCAAGCTTTATCCTACTGTAATAGGCTGCTCCAAAG83650.13807503755178846No Hit
GAACAGCCACACCAGCAAGCTTCCTGAGAGCAGAAAGTTTTGCTACTTTT83200.1373322549229982No Hit
GAACAGCCACACCAGCAAGCTTGGTTTCCTCACCTTGAAAGTGGGAATTA83130.1372167109585197No Hit
GAACAGCCACACCAGCAAGCTTGGAGACCAGTCCTAGACCTACACGCTAC83130.1372167109585197No Hit
GAACAGCCACACCAGCAAGCTTGAAAATACCTGTAGGGAGTAGGAAACTA82440.13607777759437467No Hit
GAACAGCCACACCAGCAAGCTTAGGAGAGGCTTTCTCTGTGAGGGAGGAG82440.13607777759437467No Hit
GAACAGCCACACCAGCAAGCTTGGTGTACATTGACATTTGAATTCAATGT81400.13436112440783718No Hit
GAACAGCCACACCAGCAAGCTTGATGCCTACATTATAAATGAGAAAATGG77870.12853440734199362No Hit
GAACAGCCACACCAGCAAGCTTGTAGTGGCCCTCCGTATATCTGCCCTGA77680.12822078800983774No Hit
GAACAGCCACACCAGCAAGCTTACATCATTACCAAAGAAAAAAGTAAAAT77660.12818777544855817No Hit
GAACAGCCACACCAGCAAGCTTAATTAATGCTTACAAAGTGCTCTGAGGC77220.12746149910040772No Hit
GAACAGCCACACCAGCAAGCTTGTTTATGGTGGCAAAAGATAGACACATC76800.1267682353135368No Hit
GAACAGCCACACCAGCAAGCTTACCTGTAATCCCTGCTGCTCAAACTTCA76120.12564580823003152No Hit
GAACAGCCACACCAGCAAGCTTCTGGAGGCCCTCAGCAGAAGCGAATGCC74640.12320287869534358No Hit
GAACAGCCACACCAGCAAGCTTATAACAAAAGATATAACAGAGAGTTTAT73270.12094151824769325No Hit
GAACAGCCACACCAGCAAGCTTCTGCCCTATTTCTGGCCCCACGTCCCAA72380.11947245927075253No Hit
GAACAGCCACACCAGCAAGCTTCAGGGTGGATTATTACCTGACACAAAAA71980.11881220804516117No Hit
GAACAGCCACACCAGCAAGCTTCCAGGGCCCGACTGCCCAGCACAGCCCA70980.11716157998118283No Hit
GAACAGCCACACCAGCAAGCTTCCATGGTGTTGAAGGGGACCACCGCTGC70850.11694699833286566No Hit
GAACAGCCACACCAGCAAGCTTGCTTCCTCTTGGAGCAAAAGCATTTTAG69700.11504877605929056No Hit
GAACAGCCACACCAGCAAGCTTTAACCTGAAAAATGAAATTTAGCTGCTA69580.11485070069161316No Hit
GAACAGCCACACCAGCAAGCTTCTTTGAGCCAGGCTTCTGCAAGGCTGGC68960.11382731129194659No Hit
GAACAGCCACACCAGCAAGCTTAGAGAGATTGAGGCCAATGAGAGGTGGC68930.11377779245002723No Hit
GAACAGCCACACCAGCAAGCTTCAAGATGGTATCTTTAGGATAATTACAT68270.11268837792780154No Hit
GAACAGCCACACCAGCAAGCTTGGGGCTTATCCCATTTTTGGCTTGGTGA68020.11227572091180694No Hit
GAACAGCCACACCAGCAAGCTTTGAGACGGCGCCCAGCAAAATGTCGTAT66760.11019592955119424No Hit
GAACAGCCACACCAGCAAGCTTCAGGAAAGAATAGGCTGCACAGGGGCGC66740.11016291698991466No Hit
GAACAGCCACACCAGCAAGCTTTAAAAAGCAGCGTGCAACGCAATTTCCC66690.11008038558671576No Hit
GAACAGCCACACCAGCAAGCTTTACCCTGGATGGAGAGTGTATCTCATTT66380.10956869088688247No Hit
GAACAGCCACACCAGCAAGCTTTCCAGTTTCACATCGGAAAATGGGAACA66120.1091395275902481No Hit
GAACAGCCACACCAGCAAGCTTTACTAGAAAGATGGCTCAGTAAGTAACA64960.10722479903603321No Hit
GAACAGCCACACCAGCAAGCTTGTACCCACGCTGTGCCACTCAGAAGGAA64810.10697720482643645No Hit
GAACAGCCACACCAGCAAGCTTCCAACCCCCTCCCCTCAGTCTCACACCC64790.10694419226515689No Hit
GAACAGCCACACCAGCAAGCTTGCTAGGCAAGGGCAATGGGCCTCCCAGC63890.10545862700757638No Hit
GAACAGCCACACCAGCAAGCTTGCTTCTAGGTGCCCATCTCACCCATCCT63830.10535958932373769No Hit
GAACAGCCACACCAGCAAGCTTTTCCATTTTTTCTTGCACAAAGCAGCTG63830.10535958932373769No Hit
GAACAGCCACACCAGCAAGCTTCCTCTCAATGGCAGGGGATACTCAGGGT63370.10460030041430765No Hit
GAACAGCCACACCAGCAAGCTTCAAGGAGAAGATGTGACAAGGAGAGGCC62240.10273509070201212No Hit
GAACAGCCACACCAGCAAGCTTTGAATCCTGAACTCCCAGCCCCAGCATT61290.1011669940412327No Hit
GAACAGCCACACCAGCAAGCTTTAAAATCTATTTAACTTCCAAGGCTTAA60770.10030866744796395No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)