FastQCFastQC Report
Sun 11 Sep 2022
EGAF00000709940

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000709940
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3344240
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGGTTCTCGCAAGCTTCAGGAATACAAAGCTAAAAGACAAGGTATCCC156166746.69721670693491No Hit
CGAGGTTCTCGCAAGCTTCACAGTCTGGGGAGGTGAAAGGGAAGCAAACC388711.1623268664928355No Hit
CGAGGTTCTCGCAAGCTTACAAATTTCTAGGCAAAAAGCACACTTCAGGA355261.0623041408511351No Hit
CGAGGTTCTCGCAAGCTTTCCTTTTATCACCTTTATTTTTTACCTTCCCT289870.8667739157476736No Hit
CGAGGTTCTCGCAAGCTTACAAGCAGAGAAGGAGCAGAGTGTCCGCAATC215510.6444214530057651No Hit
CGAGGTTCTCGCAAGCTTTTCACATTTTTTATATCAAGAAATAAAGCAAG193280.5779489510322225No Hit
CGAGGTTCTCGCAAGCTTGTGCCACCATGCCCAGCCAATTTTGTGTGTGT168640.5042700284668564No Hit
CGAGGTTCTCGCAAGCTTCAGGAATACAAAGCTAAAAGACAAGGAATCCC164810.49281750113628214No Hit
CGAGGTTCTCGCAAGCTTACCTATTTTTAGGATCAATCAATGTGAGGAAT134630.4025727818577614No Hit
CGAGGTTCTCGCAAGCTTATCCACCAAGATCAATCAATGTGAGGAATGAC132920.39745951247518124No Hit
CGAGGTTCTCGCAAGCTTGTAATCTCAGCTACTCAGGAGGCTGAGGCAGG113930.34067531038442217No Hit
CGAGGTTCTCGCAAGCTTTCACCTCTTGTTAAAGCAGTAAATGGTCTTTG99790.2983936559577064No Hit
CGAGGTTCTCGCAAGCTTCAGGAATACAAAGCTAAAGGACAAGGTATCCC76110.22758534076501688No Hit
CGAGGTTCTCGCAAGCTTCAGGAATACAAGGCTAAAAGACAAGGTATCCC72090.2155646723919336No Hit
CGAGGTTCTCGCAAGCTTCAGGAATACAAAGCTAAAAGGCAAGGTATCCC71240.21302298878070952No Hit
CGAGGTTCTCGCAAGCTTCAAAAATGCATAGAATTATGTAACCATTTCCA66340.19837093031600603No Hit
CGAGGTTCTCGCAAGCTTCAGGAGTACAAAGCTAAAAGACAAGGTATCCC65540.1959787575054422No Hit
CGAGGTTCTCGCAAGCTTCAGGAATACAAAGCTAAAAGACAAGGCATCCC65010.19439394301844365No Hit
CGAGGTTCTCGCAAGCTTGTGGCCAAATGTAGACAAATTTTATGCATTAT64770.1936762911752745No Hit
CGAGGTTCTCGCAAGCTTCTAGAAATAATAAGTGAGTTTAGCAAGTTAAC64770.1936762911752745No Hit
CGAGGTTCTCGCAAGCTTCAGGAATACAAAGCTAAAAGACAGGGTATCCC64390.19254000909025668No Hit
CGAGGTTCTCGCAAGCTTCAGGGATACAAAGCTAAAAGACAAGGTATCCC62080.1856326100997536No Hit
CGAGGTTCTCGCAAGCTTCAGGAATACGAAGCTAAAAGACAAGGTATCCC61520.18395808913235892No Hit
CGAGGTTCTCGCAAGCTTCAGGAATACAAAGCTAAAAGACGAGGTATCCC60240.18013061263545677No Hit
CGAGGTTCTCGCAAGCTTCAGGAATACAAAGCTAAGAGACAAGGTATCCC58260.1742099849293113No Hit
CGAGGTTCTCGCAAGCTTCGGGAATACAAAGCTAAAAGACAAGGTATCCC57050.1705918235533335No Hit
CGAGGTTCTCGCAAGCTTATCCACCATGATCAATCAATGTGAGGAATGAC56380.1685883788244863No Hit
CGAGGTTCTCGCAAGCTTGAGGAGCAAGGTAATAAAGTGGCACCTTAAAA56270.16825945506303375No Hit
CGAGGTTCTCGCAAGCTTCAGGAATACAGAGCTAAAAGACAAGGTATCCC54900.1641628591249432No Hit
CGAGGTTCTCGCAAGCTTTTTGATGTGCTGCTGGATTCGGTTTGCCAGTA54880.16410305480467907No Hit
CGAGGTTCTCGCAAGCTTGTGGGGGTTATATCCTACTGCTCAAGGTCATC53510.1600064588665885No Hit
CGAGGTTCTCGCAAGCTTCAGGAATACAAAGCTAAAAGACAAGGTACCCC53360.1595579264646078No Hit
CGAGGTTCTCGCAAGCTTAGCGTTCCAATAACGGAACACTAGGCATAATG53300.15937851350381552No Hit
CGAGGTTCTCGCAAGCTTTGGTGGCCGAAATTCTCGTATGCCGTCTTCTG52990.15845154653972202TruSeq Adapter, Index 19 (95% over 24bp)
CGAGGTTCTCGCAAGCTTCACGAATACAAAGCTAAAAGACAAGGTATCCC52240.15620888452981843No Hit
CGAGGTTCTCGCAAGCTTTTTGAAAGATGGGATACCTTGTCTTTTAGCTT49240.14723823649020407No Hit
CGAGGTTCTCGCAAGCTTCAGGAATACTAAGCTAAAAGACAAGGTATCCC47870.14314164055211348No Hit
CGAGGTTCTCGCAAGCTTTTGTAGATGCCTTAAAGTTTTGTATGTAGACA47730.14272301031026483No Hit
CGAGGTTCTCGCAAGCTTTCCTTGCTCCAGAGTGAGACACTATCTCCATC44560.13324402554840561No Hit
CGAGGTTCTCGCAAGCTTCAGGAATACAAAGCTAGAAGACAAGGTATCCC44460.13294500394708514No Hit
CGAGGTTCTCGCAAGCTTCAGGAATTCAAAGCTAAAAGACAAGGTATCCC41800.1249910293519604No Hit
CGAGGTTCTCGCAAGCTTTTACTCAAGGTACAGTTACATGATAAGCAAAA40250.12035619453149296No Hit
CGAGGTTCTCGCAAGCTTTCTGGAAAATTGATGAATATTTGAAAATTAAT38830.11611008779274215No Hit
CGAGGTTCTCGCAAGCTTCAGGAATACAAAGCTGAAAGACAAGGTATCCC37160.11111642705069014No Hit
CGAGGTTCTCGCAAGCTTCAGGAATGCAAAGCTAAAAGACAAGGTATCCC36130.10803650455708921No Hit
CGAGGTTCTCGCAAGCTTAGTTTGGCTGGATATGAAATTCTGGGTTGAAA35850.10719924407339186No Hit
CGAGGTTCTCGCAAGCTTCAGGAATACAAAGCTAAAAGACAAGGTATCCA34700.10376049565820634No Hit
CGAGGTTCTCGCAAGCTTCAGGAATACAAAGCCAAAAGACAAGGTATCCC34650.1036109848575461No Hit
CGAGGTTCTCGCAAGCTTCAGGAATACAAAGCTAAAAGACAAGGTGTCCC34110.10199626821041552No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)