FastQCFastQC Report
Sat 3 Sep 2022
EGAF00000709944

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000709944
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences741932
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAACCTAATCAAGCTTTTATCACAATGAAAGAAATACTCAAAGCATG571467.702323123952061No Hit
ACCTAACCTAATCAAGCTTCCCGTTTTTCTAGGTGAGTGTCCCAAAGTTA189182.549829364416146No Hit
ACCTAACCTAATCAAGCTTTCCTAGGGTGCTACACAGATATATAAAGCTG175062.3595154272898324No Hit
ACCTAACCTAATCAAGCTTGCAAAAGGAGATCCCTATTTTACTTTTACGT162552.1909015920596495No Hit
ACCTAACCTAATCAAGCTTGAGCTCCACCGAAACCAGCACGGAATTTCTA154792.0863097965851316No Hit
ACCTAACCTAATCAAGCTTGTAAAGAATTTTGTGAGCTGAAGTATTTTAA131121.767277863739534No Hit
ACCTAACCTAATCAAGCTTTGGAGGACAAGATGTGCTGATCCCTATTTTA129821.7497560423327205No Hit
ACCTAACCTAATCAAGCTTAACTCCCTGACAAAACTAGGAATTCCCTGAA110151.4846374061234724No Hit
ACCTAACCTAATCAAGCTTGATCCCTATTTTACTTTTACGTGCTTAATCC52490.7074772351104953No Hit
ACCTAACCTAATCAAGCTTTTCTGCTTCTTAGGGGGCTTTAATCTAAAGA49900.672568375538459No Hit
ACCTAACCTAATCAAGCTTATACATAGTAGAGCAAGGAATTGAACCTAAA45450.6125898330305203No Hit
ACCTAACCTAATCAAGCTTATCCACCATGATCCCTATTTTACTTTTACGT45420.6121854833057476No Hit
ACCTAACCTAATCAAGCTTATCCACCACGATCCCTATTTTACTTTTACGT39350.5303720556600875No Hit
ACCTAACCTAATCAAGCTTCCAACTAATGAGAATGAATCTCATCAATGTC36260.4887240340085075No Hit
ACCTAACCTAATCAAGCTTGTTTTCAGCCAGTGTAAGCGTGGTGTTCTTG35030.4721456952928301No Hit
ACCTAACCTAATCAAGCTTACCTCATTTTGGTTTTGTCAATAAAGGGTCA33590.4527369085037443No Hit
ACCTAACCTAATCAAGCTTCTTGCAGCACCTTTTTTTCACTGTCGCATCA31060.41863674838125325No Hit
ACCTAACCTAATCAAGCTTCAAATGGGAAGGATCCCTATTTTACTTTTAC30520.4113584533353461No Hit
ACCTAACCTAATCAAGCTTAGTTTGGCTGGATATGAAATTCTGGGTTGAA29560.39841926214262224No Hit
ACCTAACCTAATCAAGCTTATCCACCAGGATCCCTATTTTACTTTTACGT27450.36997999816694793No Hit
ACCTAACCTAATCAAGCTTATTTTGAGGGTAGCAACATGGCGTTCCCAAC26030.35084077786104384No Hit
ACCTAACCTAATCAAGCTTGCATGTCTGCCTTCTTCCTCATAGAAACACT25720.34666249737172683No Hit
ACCTAACCTAATCAAGCTTTGAACCTAAGTTGAACCTTCCAACCCCGGCA24210.3263102278915049No Hit
ACCTAACCTAATCAAGCTTGCTATGATCCCTATTTTACTTTTACGTGCTT22700.305957958411283No Hit
ACCTAACCTAATCAAGCTTTTTTTATTTAAAAAAACCCAATAACTCAGTT22650.3052840422033286No Hit
ACCTAACCTAATCAAGCTTGATGCAGTGGTTCACACCTGTAATCCCATCA21550.2904578856283325No Hit
ACCTAACCTAATCAAGCTTTGAGGTCTGCACATCAGATAGCATTACTCCC20950.2823708911328801No Hit
ACCTAACCTAATCAAGCTTTCTTAGCACAGAAGGAGTAAACTGCAAATGA20530.2767099949860634No Hit
ACCTAACCTAATCAAGCTTCTCCACAGTGAAGGAAACAATAAACAGACTA20310.27374476367106415No Hit
ACCTAACCTAATCAAGCTTCCAGCACCAGACAGGGAAGAAGGAGTTCTGA19830.26727516807470225No Hit
ACCTAACCTAATCAAGCTTCGTCTTCATGAAACTTATATTTTAGAAGGGG19740.2660621189003844No Hit
ACCTAACCTAATCAAGCTTATCGACCATGATCCCTATTTTACTTTTACGT18700.25204466177493356No Hit
ACCTAACCTAATCAAGCTTTCATGCTATGTTCAGTAAGGCAGAATCAAAT18030.24301418458834503No Hit
ACCTAACCTAATCAAGCTTCATAAGTGAAGGAGAAATAAAATCCTTTACA17440.2350619733344835No Hit
ACCTAACCTAATCAAGCTTCACAGCTGCTGATGGACACAGACACTGGGAC16980.22886194422130332No Hit
ACCTAACCTAATCAAGCTTTACAGTGGCTCCATTTCACTCAGGCTCACCA16840.22697497883903106No Hit
ACCTAACCTAATCAAGCTTCAGAACACCTTGGAATACAGTTTAGCTTCCT16690.22495323021516797No Hit
ACCTAACCTAATCAAGCTTGCATGTGTTTGAAAAAGATCCCTATTTTACT16400.22104451620903265No Hit
ACCTAACCTAATCAAGCTTACTGTCCCATGAATTATCATAATATGACAAA16300.2196966837931239No Hit
ACCTAACCTAATCAAGCTTCTGAAAGATCCCTATTTTACTTTTACGTGCT16260.21915755082676042No Hit
ACCTAACCTAATCAAGCTTCTCTGTTCCCTGATGCAGTGTGGATATTTTT15910.2144401373710798No Hit
ACCTAACCTAATCAAGCTTCCAGGATAAGCCTCGGGGATCCCTATTTTAC15650.2109357730897171No Hit
ACCTAACCTAATCAAGCTTCCAGAGAAAGGATCCCTATTTTACTTTTACG15430.2079705417747179No Hit
ACCTAACCTAATCAAGCTTTAGAAAACTCTTTTTTCTTATCAGAATCTAG15400.20756619204994525No Hit
ACCTAACCTAATCAAGCTTAACAGGCAGGACATTAGAGTCCAGGGGGGCA15340.20675749260040005No Hit
ACCTAACCTAATCAAGCTTAATGCAGAAAATATACCCAAGTCAGGAGGTA15290.20608357639244568No Hit
ACCTAACCTAATCAAGCTTCATAAGTGAAGGAGAAATAAAATACTTTACA14990.20204007914471944No Hit
ACCTAACCTAATCAAGCTTTGGAGTCATATCTGGGCCTGTATCTGTCTCT14840.20001833052085638No Hit
ACCTAACCTAATCAAGCTTCAGTAGCCAATTCGATAAAGTGGAAGAAAGG14840.20001833052085638No Hit
ACCTAACCTAATCAAGCTTCCTCTTGGGAGAATTAGGGAAGTTTCAAGGC14580.19651396623949366No Hit
ACCTAACCTAATCAAGCTTTGATTTTTGCAGCTCCCCTGAAGATTTGGAA14570.19637918299790277No Hit
ACCTAACCTAATCAAGCTTGAGTCTGGGGAGAAGGATGGTGGGAAAGAAA14480.19516613382358491No Hit
ACCTAACCTAATCAAGCTTGGGCCTGTGATGTTCATCTCAGCCCCATGTG14130.19044872036790433No Hit
ACCTAACCTAATCAAGCTTCCCTCTACAACATCCTCTCTTTCCAGCTGTG13930.18775305553608687No Hit
ACCTAACCTAATCAAGCTTTAGTGTGCTCAGAATGACCAGAGAACCTTGT13880.1870791393281325No Hit
ACCTAACCTAATCAAGCTTTGGCTCTCCTGATCCCTATTTTACTTTTACG13760.185461740429042No Hit
ACCTAACCTAATCAAGCTTTCTATTTTGTTAAATCTTTTTATGCTTGGCT13560.18276607559722455No Hit
ACCTAACCTAATCAAGCTTAAAGTGTCAATTTTTATACTTATCTGGTTTG12930.1742747313769995No Hit
ACCTAACCTAATCAAGCTTTTAGATGTCTCTGTTTACTTTGATCCCTATT12830.17292689896109076No Hit
ACCTAACCTAATCAAGCTTCTAGGGAGCAGAGATGATGGTGTAATTCCTT12540.16901818495495544No Hit
ACCTAACCTAATCAAGCTTGTCTATGAACTGAAGGAAGAAAGATGGGACC12330.1661877368815471No Hit
ACCTAACCTAATCAAGCTTCAATTATAAAATGAATGACAGATGCACAAGA12280.16551382067359272No Hit
ACCTAACCTAATCAAGCTTAGCGTAGCACTATTTGTGGTCAGATCCCTAT12150.16376163853291137No Hit
ACCTAACCTAATCAAGCTTAGCAACATATTCTGAGTCATCATGGGAGAAT11800.1590442250772308No Hit
ACCTAACCTAATCAAGCTTTTAGTTGGAAAGATTGTTCAATGATCCCTAT11730.15810074238609467No Hit
ACCTAACCTAATCAAGCTTGTCAAGTGTTTAGCTCTGTGCCTGGGAAATA11590.15621377700382244No Hit
ACCTAACCTAATCAAGCTTCAATATCTGAGGGCAGGAGAAGGTAGATATC11340.1528441959640506No Hit
ACCTAACCTAATCAAGCTTTCCCCAGGCCAAGGCACCAGCTGCTGCTCCA11240.1514963635481419No Hit
ACCTAACCTAATCAAGCTTACCCAGTGTGTTAGGCATAATACTAGCCCTC11160.1504180976154149No Hit
ACCTAACCTAATCAAGCTTTACAATACCCTGATATATAGGAATTATACTA11000.1482615657499609No Hit
ACCTAACCTAATCAAGCTTGGATTATCCAGGAGGGAAACCTGACAATATA10700.1442180685022347No Hit
ACCTAACCTAATCAAGCTTCACTTCACTAAACAGAGCTAGTTTGATTTCT10470.1411180539456446No Hit
ACCTAACCTAATCAAGCTTAGCTTATTAATTATTTCTTTCATGGATCCCT10350.13950065504655412No Hit
ACCTAACCTAATCAAGCTTCTGTTGAAGGCAATCCTACTGGAACTTTTAG10180.13720933993950926No Hit
ACCTAACCTAATCAAGCTTTAAACCTTTTGGAATGTAAAGGCATATTTGT10000.13478324159087357No Hit
ACCTAACCTAATCAAGCTTAGTCCTGAGACCCGGGGAGATTAAAAAAAAA10000.13478324159087357No Hit
ACCTAACCTAATCAAGCTTTTAACATAGGGAGCCCCTTATTCACCATAAG9860.13289627620860134No Hit
ACCTAACCTAATCAAGCTTTTGCTTTCACTCCAATTAAAACAACAATGGT9620.12966147841042036No Hit
ACCTAACCTAATCAAGCTTCCAACTTACAGTTCAATATCCAGAGAAAATA9530.1284484292361025No Hit
ACCTAACCTAATCAAGCTTCCCCCACCTGCTATGGTGATGACATCGATCC9260.12480928171314891No Hit
ACCTAACCTAATCAAGCTTAGCAAGAATTTTAAATTACTGAGTAAATGAA8980.12103535094860444No Hit
ACCTAACCTAATCAAGCTTGAACAACAGGGTCTTCCAACCAGGGGTTTGA8980.12103535094860444No Hit
ACCTAACCTAATCAAGCTTTATACCAGATGAAGTAAATAGAAAAGAAGAA8980.12103535094860444No Hit
ACCTAACCTAATCAAGCTTCATCTGTATTTAGCCACTCCCCATGGCTTGC8950.12063100122383183No Hit
ACCTAACCTAATCAAGCTTCTGTGTTCCGAATTAGACACATCTTTATTTA8940.12049621798224096No Hit
ACCTAACCTAATCAAGCTTGGGTACAGCCTGCAGCCAGTGTCCCATCTGA8850.11928316880792311No Hit
ACCTAACCTAATCAAGCTTTCTTCATTCTTTTTTATTCTGTTTTCTCCTC8820.11887881908315048No Hit
ACCTAACCTAATCAAGCTTAGAGGACATTGTGGAGATGTTAACTGGCCTA8820.11887881908315048No Hit
ACCTAACCTAATCAAGCTTTTTGATGTGCTGCTGGATTCGGTTTGCCAGT8690.11712663694246911No Hit
ACCTAACCTAATCAAGCTTTCAAAACAGAAAGCACCAGCCCAAATGAATT8680.11699185370087825No Hit
ACCTAACCTAATCAAGCTTAGTTTGGCTGGATATGAAATTCTGAGTTGAA8640.11645272073451476No Hit
ACCTAACCTAATCAAGCTTAGAAGGTCCCACTTTACATGTGGAGGCTCAG8600.11591358776815126No Hit
ACCTAACCTAATCAAGCTTAATCAGGCAGGACATTAGAGTCCAGGGGGGC8600.11591358776815126No Hit
ACCTAACCTAATCAAGCTTTCAGGACCCCTCACTTACACTGACACCTTCT8590.11577880452656039No Hit
ACCTAACCTAATCAAGCTTACCTTAAGCCTAAGAGTTATATACAATTATT8550.11523967156019689No Hit
ACCTAACCTAATCAAGCTTCATTATGTAGGCATGATTGATTAAATCATTA8520.11483532183542426No Hit
ACCTAACCTAATCAAGCTTCCAGAGGAAGGATCCCTATTTTACTTTTACG8460.11402662238587903No Hit
ACCTAACCTAATCAAGCTTTGGTCTGGAACACAGTTATCTGGAAATAGTT8450.11389183914428816No Hit
ACCTAACCTAATCAAGCTTAGTCATCTGACTTAGAAAGGACAGAAACTAT8340.11240922348678854No Hit
ACCTAACCTAATCAAGCTTTAAGCCAAGCAAAAGAGTTTGGATTTTTTCC8280.11160052403724331No Hit
ACCTAACCTAATCAAGCTTACCCTAAGATCCCTATTTTACTTTTACGTGC8210.11065704134610718No Hit
ACCTAACCTAATCAAGCTTGAATCTAGGATCCCTATTTTACTTTTACGTG8140.10971355865497107No Hit
ACCTAACCTAATCAAGCTTCCTAAATCATTAAAAGGATATCCTTTTAAAG8120.10944399217178932No Hit
ACCTAACCTAATCAAGCTTACAGGCTTGCCTTCTGAATTTTTTCCACCTC8030.10823094299747148No Hit
ACCTAACCTAATCAAGCTTGCCAACCTCTCCTCTAATCTAGTCTGCCTTG8020.10809615975588059No Hit
ACCTAACCTAATCAAGCTTTAATGGATTTTAGTCCACATTATTTTAGTAA7960.10728746030633535No Hit
ACCTAACCTAATCAAGCTTGCCCAGCACCAAGCAGTCATATAGGCCACAA7860.10593962789042662No Hit
ACCTAACCTAATCAAGCTTACTAAATTTTTCAGTTAATGATGGTGGCATT7840.10567006140724487No Hit
ACCTAACCTAATCAAGCTTCAGTAGGCCAGGCGCAGTGGCTCATGCCTGT7810.10526571168247224No Hit
ACCTAACCTAATCAAGCTTGTTGTCATCAAATAAAACGCACAGCAAAGCT7770.10472657871610876No Hit
ACCTAACCTAATCAAGCTTAGAGAGCACTGAACGGGATAAATGGCCAAAG7760.10459179547451788No Hit
ACCTAACCTAATCAAGCTTTTTGATGTGTTACTGGATTTGGCATTTGGAA7710.10391787926656351No Hit
ACCTAACCTAATCAAGCTTCATTAAAATTAAATCCTTTTTCTCTGGGGAA7690.10364831278338177No Hit
ACCTAACCTAATCAAGCTTCCAAATCAGGTGACTTTCAGTTAATAAAAGA7570.1020309138842913No Hit
ACCTAACCTAATCAAGCTTAAGAGCTTCTACACAGCAAAAGAAACTATTA7510.10122221443474604No Hit
ACCTAACCTAATCAAGCTTTGGTAACTGAGCTCCTCACTGCTCCAAGTTC7480.10081786470997342No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)