FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000709945

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000709945
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5525134
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTGTATTACCAAGCTTAAGAAATAAAACAGTTGAGTGATCTCTGGCTA121418721.975702308758486No Hit
GACTGTATTACCAAGCTTCCCTCTGGTTTGTAGCTCAACATTAATGAGTT1174962.126572857780463No Hit
GACTGTATTACCAAGCTTCTTTCTCAAAACAGGGCTCAGGCCTAGAAAAA797491.4433858074754387No Hit
GACTGTATTACCAAGCTTCTTGTAGAAAAGATCTCTGGCTATCCTTGCCA607091.098778780749933No Hit
GACTGTATTACCAAGCTTCATGCAAATGTGAACTTGAACTCTGCTCTTAG234770.4249127713463601No Hit
GACTGTATTACCAAGCTTAATCCATGTAAAGCTATTATTATTGTGCTGCT213680.38674175142177547No Hit
GACTGTATTACCAAGCTTTGTGCTGTGTTAAAACCAGGAAGGTATCTACG203600.3684978500068958No Hit
GACTGTATTACCAAGCTTTCCCACCTACCTACCCAGAGCAGAAGGAAGGT200040.36205456736433905No Hit
GACTGTATTACCAAGCTTTCAGTCCCATGTTAGACAGTGTGATTAGAGGA191320.34627214471178436No Hit
GACTGTATTACCAAGCTTACCTGGGACAGTCCTAGTGAAGCCACCACTTT187970.34020894334870433No Hit
GACTGTATTACCAAGCTTACAATGAAGTTTTGTGTTGAGTTATAACATTC184510.33394665179161265No Hit
GACTGTATTACCAAGCTTAAGAAATAAAACAGTTGAGTGATCAGTGATTA178450.322978592012429No Hit
GACTGTATTACCAAGCTTGAATATTGAACTTAGGTGGGAGGTATTATTCC166460.30127776086516633No Hit
GACTGTATTACCAAGCTTAAGAAATAAAACAGTTGAGTTATCTCTGGCTA159260.28824640271168084No Hit
GACTGTATTACCAAGCTTGATGAAGACCAATGCAATATGTTTCACAGCCA157800.28560393286389074No Hit
GACTGTATTACCAAGCTTCTCTCTGCCTTCTGGGCTTCAAGGCCAGGCCT153340.2775317304521483No Hit
GACTGTATTACCAAGCTTTGGCAAGATTTGGTATCTGTAGCAGACTCCAG150180.2718124121514519No Hit
GACTGTATTACCAAGCTTTCTCTGTAGCACCCCCACTCTTCCTACACACT146690.26549582326872073No Hit
GACTGTATTACCAAGCTTCCTGTTATCAGTGGGTGGGCACTCGCAAAGAG146530.2652062375319766No Hit
GACTGTATTACCAAGCTTGACCGTGGCTCCTCATTCTGTGGAGTGACGAT144220.2610253434577333No Hit
GACTGTATTACCAAGCTTTCTCACCATTAATCCTGTGGTCTGAGGGCCTT138050.24985819348453808No Hit
GACTGTATTACCAAGCTTTTTCAGGGTTGCATCATTTGGCCCTATTCACC136110.2463469664265156No Hit
GACTGTATTACCAAGCTTAGGGTAATGTATGGGGTCACCAAGGAGATGAG135790.2457677949530274No Hit
GACTGTATTACCAAGCTTTCAGAATGGAACGCAATGAGAGCCAGGTGGCT133140.24097153118820286No Hit
GACTGTATTACCAAGCTTGGGTGTTGGATAACCCATTCAAGACACAGGTG130160.2355779968413436No Hit
GACTGTATTACCAAGCTTTTTCTATGGTCCGGTGCTGTGCAGATTATCTC127810.23132470633291427No Hit
GACTGTATTACCAAGCTTAATATAAGAGAATCTAGATTTACAGAAGAAGA124980.22620265861425262No Hit
GACTGTATTACCAAGCTTCCTGCAAAACGGGAGGAGCGTATTGATCTCTG122440.2216054850434397No Hit
GACTGTATTACCAAGCTTTTGGGGCAAAGGCAATATAAATTATGCCCTTG121060.21910780806402164No Hit
GACTGTATTACCAAGCTTTTGGGTCAAAGGCAATATAAATTATGCCCTTG119970.21713500523245227No Hit
GACTGTATTACCAAGCTTACATTCTAGCAGAGAAAAATGAATCAATTATA119070.21550608546326658No Hit
GACTGTATTACCAAGCTTGGGCAGAGGCAACAGGAAGTGGGAAATGAGTC117450.21257402987873236No Hit
GACTGTATTACCAAGCTTCTGCAGAGTTCTTTTGGGACACTCTCTCTGGG117260.21223014681634872No Hit
GACTGTATTACCAAGCTTAGTTTGGCTGGATATGAAATTCTGGGTTGAAA110000.19909019401158418No Hit
GACTGTATTACCAAGCTTCCTTATCTCCCATCTAAAGCTATCTGTGAACA106730.19317178551687614No Hit
GACTGTATTACCAAGCTTGTTACAAGTGCAGAACCCAACCCTATACAGTC105740.1913799737707719No Hit
GACTGTATTACCAAGCTTTTCATATTCTCAAATATTCACTGTTTAATCAA105310.19060171210327206No Hit
GACTGTATTACCAAGCTTCAAGACGATTCCAAAGGAGCCCAACGGCCAAG101840.1843213214376339No Hit
GACTGTATTACCAAGCTTAAACTAGAATTACCATATGATCTCTGGCTATC99240.1796155532155419No Hit
GACTGTATTACCAAGCTTAAAGTCTCATTGGGCTCCTATGTTCAAAAGGA98880.17896398530786764No Hit
GACTGTATTACCAAGCTTAACATCACGCTCTCTCGGTAGAAAGGAGACAT98390.17807712898908878No Hit
GACTGTATTACCAAGCTTTATCATACTAGTAACTGCAGTTATTAAAATTA98240.17780564236089114No Hit
GACTGTATTACCAAGCTTCCTACTGCTCTGAGGTCTTAAGGGATGTTCTG97610.17666539852246116No Hit
GACTGTATTACCAAGCTTATCCACCATGATCTCTGGCTATCCTTGCCATT95810.1734075589840898No Hit
GACTGTATTACCAAGCTTTCCTCTAACTTACTTTCCCAAGGCAGAGAGAA94140.17038500785682303No Hit
GACTGTATTACCAAGCTTGTTACAAGTGCAGACCCCAACCCTATACAGTC90190.1632358599809525No Hit
GACTGTATTACCAAGCTTCGTGTCTCTGCATTCTCGGAATCAGTCACACA85720.15514555846066358No Hit
GACTGTATTACCAAGCTTCATGAGGGTAAGTCCTGTTAGTTTATTTTTTT83930.15190581803083872No Hit
GACTGTATTACCAAGCTTTTAAACTTTTTGGACTTTAACCTACAATAAGA83100.15040359202147857No Hit
GACTGTATTACCAAGCTTTTATTTGTAGACATTTGTGCCTGTTCTTATAA81270.1470914551574677No Hit
GACTGTATTACCAAGCTTCATTCATTTCATGGGGAGAAATGACATATCTC80590.14586071577630516No Hit
GACTGTATTACCAAGCTTCATGAGGGTAAGGCCTGTTAGTTTATTTTTTT78510.14209610119863156No Hit
GACTGTATTACCAAGCTTAAGAAATAAAACAGTTTAGTGATCTCTGGCTA76170.13786090979874877No Hit
GACTGTATTACCAAGCTTAAGAAATAAAACAGTTTAGTTATCTCTGGCTA70320.1272729312990418No Hit
GACTGTATTACCAAGCTTCCTGGAAGCCCAGGGGAGAAGGAAAATGGGTT68260.12354451493846122No Hit
GACTGTATTACCAAGCTTGGCCATTGACCTCAGAAATCCTCGTATGCCGT66040.11952651284113651No Hit
GACTGTATTACCAAGCTTAGGGGGATCTCTGGCTATCCTTGCCATTGACC64770.11722792605573006No Hit
GACTGTATTACCAAGCTTCTTTAAGTAAGTCATGGGCTCACCATCTGGAA64340.11644966438823022No Hit
GACTGTATTACCAAGCTTAGCTTCTGGGGCAGGCAGATCTCTGGCTATCC64170.11614197954293959No Hit
GACTGTATTACCAAGCTTCAGGCTTTGAGGGTGAACTACTTGGAGTTACA62670.11342711326096346No Hit
GACTGTATTACCAAGCTTTCAGATGATACCGTTCCTGCTGGCCCATGGAT62450.11302893287294027No Hit
GACTGTATTACCAAGCTTGTTCCTTACAGATAACTAGAATGTCGATTTTA60040.10866704771323193No Hit
GACTGTATTACCAAGCTTGCCTGGTGTCCCAGAAAACGATAAAGAGGGTC59830.10828696643375527No Hit
GACTGTATTACCAAGCTTAAGAAATAAAACAGGTGAGTGATCTCTGGCTA59020.10682093864148814No Hit
GACTGTATTACCAAGCTTAAGAAATAAAACAGTTGAGTGATCTCTGTCTA57610.10426896433643057No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)