FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000709946

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000709946
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1001065
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAACCTAATCAAGCTTTTATCACAATGAAAGAAATACTCAAAGCATG706477.057184098934635No Hit
ACCTAACCTAATCAAGCTTCCCGTTTTTCTAGGTGAGTGTCCCAAAGTTA269312.690234899831679No Hit
ACCTAACCTAATCAAGCTTTCCTAGGGTGCTACACAGATATATAAAGCTG246562.462976929570008No Hit
ACCTAACCTAATCAAGCTTGCAAAAGGAGATCCCTATTTTACTTTTACGT233172.329219381358853No Hit
ACCTAACCTAATCAAGCTTGAGCTCCACCGAAACCAGCACGGAATTTCTA202952.0273408819607117No Hit
ACCTAACCTAATCAAGCTTTGGAGGACAAGATGTGCTGATCCCTATTTTA186091.8589202499338204No Hit
ACCTAACCTAATCAAGCTTGTAAAGAATTTTGTGAGCTGAAGTATTTTAA182111.8191625918396908No Hit
ACCTAACCTAATCAAGCTTAACTCCCTGACAAAACTAGGAATTCCCTGAA146711.4655392007512No Hit
ACCTAACCTAATCAAGCTTGATCCCTATTTTACTTTTACGTGCTTAATCC78100.7801691198873201No Hit
ACCTAACCTAATCAAGCTTTTCTGCTTCTTAGGGGGCTTTAATCTAAAGA69990.699155399499533No Hit
ACCTAACCTAATCAAGCTTATCCACCATGATCCCTATTTTACTTTTACGT64570.6450130610899392No Hit
ACCTAACCTAATCAAGCTTATACATAGTAGAGCAAGGAATTGAACCTAAA63360.6329259338804173No Hit
ACCTAACCTAATCAAGCTTATCCACCACGATCCCTATTTTACTTTTACGT58020.5795827443772382No Hit
ACCTAACCTAATCAAGCTTCCAACTAATGAGAATGAATCTCATCAATGTC48780.487281045686344No Hit
ACCTAACCTAATCAAGCTTACCTCATTTTGGTTTTGTCAATAAAGGGTCA45660.456114238336172No Hit
ACCTAACCTAATCAAGCTTGTTTTCAGCCAGTGTAAGCGTGGTGTTCTTG44480.4443267919665556No Hit
ACCTAACCTAATCAAGCTTCAAATGGGAAGGATCCCTATTTTACTTTTAC43970.4392322176881621No Hit
ACCTAACCTAATCAAGCTTCTTGCAGCACCTTTTTTTCACTGTCGCATCA43610.43563604760929614No Hit
ACCTAACCTAATCAAGCTTAGTTTGGCTGGATATGAAATTCTGGGTTGAA41370.41325987822968535No Hit
ACCTAACCTAATCAAGCTTATCCACCAGGATCCCTATTTTACTTTTACGT39720.39677743203488286No Hit
ACCTAACCTAATCAAGCTTATTTTGAGGGTAGCAACATGGCGTTCCCAAC36410.36371264603197595No Hit
ACCTAACCTAATCAAGCTTGCATGTCTGCCTTCTTCCTCATAGAAACACT36140.3610155184728264No Hit
ACCTAACCTAATCAAGCTTGCTATGATCCCTATTTTACTTTTACGTGCTT33140.33104743448227636No Hit
ACCTAACCTAATCAAGCTTTGAACCTAAGTTGAACCTTCCAACCCCGGCA32730.3269517963369012No Hit
ACCTAACCTAATCAAGCTTTTTTTATTTAAAAAAACCCAATAACTCAGTT31420.31386573299436105No Hit
ACCTAACCTAATCAAGCTTGATGCAGTGGTTCACACCTGTAATCCCATCA30260.3022780738513483No Hit
ACCTAACCTAATCAAGCTTTGAGGTCTGCACATCAGATAGCATTACTCCC29230.29198903168125945No Hit
ACCTAACCTAATCAAGCTTCTCCACAGTGAAGGAAACAATAAACAGACTA28780.28749381908267696No Hit
ACCTAACCTAATCAAGCTTCCAGCACCAGACAGGGAAGAAGGAGTTCTGA28640.28609530849645126No Hit
ACCTAACCTAATCAAGCTTATCGACCATGATCCCTATTTTACTTTTACGT26730.26701562835580106No Hit
ACCTAACCTAATCAAGCTTCGTCTTCATGAAACTTATATTTTAGAAGGGG26680.26651616028929187No Hit
ACCTAACCTAATCAAGCTTTCTTAGCACAGAAGGAGTAAACTGCAAATGA25480.25452892669307187No Hit
ACCTAACCTAATCAAGCTTTCATGCTATGTTCAGTAAGGCAGAATCAAAT24930.24903477796147105No Hit
ACCTAACCTAATCAAGCTTGCATGTGTTTGAAAAAGATCCCTATTTTACT24300.24274148032345555No Hit
ACCTAACCTAATCAAGCTTCACAGCTGCTGATGGACACAGACACTGGGAC24010.23984456553770234No Hit
ACCTAACCTAATCAAGCTTCTGAAAGATCCCTATTTTACTTTTACGTGCT23690.23664796991204368No Hit
ACCTAACCTAATCAAGCTTTACAGTGGCTCCATTTCACTCAGGCTCACCA23660.23634828907213817No Hit
ACCTAACCTAATCAAGCTTCCAGGATAAGCCTCGGGGATCCCTATTTTAC23450.23425052319279968No Hit
ACCTAACCTAATCAAGCTTCATAAGTGAAGGAGAAATAAAATCCTTTACA22950.22925584252770798No Hit
ACCTAACCTAATCAAGCTTACTGTCCCATGAATTATCATAATATGACAAA22440.22416126824931448No Hit
ACCTAACCTAATCAAGCTTAACAGGCAGGACATTAGAGTCCAGGGGGGCA22280.22256297043648515No Hit
ACCTAACCTAATCAAGCTTCCAGAGAAAGGATCCCTATTTTACTTTTACG22130.22106456623695764No Hit
ACCTAACCTAATCAAGCTTTAGAAAACTCTTTTTTCTTATCAGAATCTAG21000.20977658793385046No Hit
ACCTAACCTAATCAAGCTTCAGAACACCTTGGAATACAGTTTAGCTTCCT20980.20957680070724677No Hit
ACCTAACCTAATCAAGCTTAATGCAGAAAATATACCCAAGTCAGGAGGTA20900.2087776518008321No Hit
ACCTAACCTAATCAAGCTTTGGAGTCATATCTGGGCCTGTATCTGTCTCT20530.20508158810866425No Hit
ACCTAACCTAATCAAGCTTTGATTTTTGCAGCTCCCCTGAAGATTTGGAA20530.20508158810866425No Hit
ACCTAACCTAATCAAGCTTCTCTGTTCCCTGATGCAGTGTGGATATTTTT20390.20368307752243858No Hit
ACCTAACCTAATCAAGCTTCAGTAGCCAATTCGATAAAGTGGAAGAAAGG20100.20078616273668543No Hit
ACCTAACCTAATCAAGCTTCCTCTTGGGAGAATTAGGGAAGTTTCAAGGC19630.19609116291149925No Hit
ACCTAACCTAATCAAGCTTCCCTCTACAACATCCTCTCTTTCCAGCTGTG19400.19379360980555707No Hit
ACCTAACCTAATCAAGCTTTGGCTCTCCTGATCCCTATTTTACTTTTACG19370.1934939289656516No Hit
ACCTAACCTAATCAAGCTTCATAAGTGAAGGAGAAATAAAATACTTTACA19350.1932941417390479No Hit
ACCTAACCTAATCAAGCTTGAGTCTGGGGAGAAGGATGGTGGGAAAGAAA19280.19259488644593506No Hit
ACCTAACCTAATCAAGCTTTTAGATGTCTCTGTTTACTTTGATCCCTATT19180.19159595031291676No Hit
ACCTAACCTAATCAAGCTTTAGTGTGCTCAGAATGACCAGAGAACCTTGT19030.19009754611338925No Hit
ACCTAACCTAATCAAGCTTGGGCCTGTGATGTTCATCTCAGCCCCATGTG19020.1899976525000874No Hit
ACCTAACCTAATCAAGCTTTCTATTTTGTTAAATCTTTTTATGCTTGGCT18610.18590201435471224No Hit
ACCTAACCTAATCAAGCTTCAATTATAAAATGAATGACAGATGCACAAGA17820.17801041890386737No Hit
ACCTAACCTAATCAAGCTTAGCGTAGCACTATTTGTGGTCAGATCCCTAT17280.17261616378556838No Hit
ACCTAACCTAATCAAGCTTCTAGGGAGCAGAGATGATGGTGTAATTCCTT16710.16692222782736388No Hit
ACCTAACCTAATCAAGCTTAAAGTGTCAATTTTTATACTTATCTGGTTTG16570.16552371724113818No Hit
ACCTAACCTAATCAAGCTTAGCTTATTAATTATTTCTTTCATGGATCCCT16460.164424887494818No Hit
ACCTAACCTAATCAAGCTTTTAGTTGGAAAGATTGTTCAATGATCCCTAT16390.1637256322017052No Hit
ACCTAACCTAATCAAGCTTAGCAACATATTCTGAGTCATCATGGGAGAAT16270.16252690884208318No Hit
ACCTAACCTAATCAAGCTTCAATATCTGAGGGCAGGAGAAGGTAGATATC15480.15463531339123832No Hit
ACCTAACCTAATCAAGCTTTACAATACCCTGATATATAGGAATTATACTA15460.15443552616463466No Hit
ACCTAACCTAATCAAGCTTGTCAAGTGTTTAGCTCTGTGCCTGGGAAATA15300.15283722835180533No Hit
ACCTAACCTAATCAAGCTTTCCCCAGGCCAAGGCACCAGCTGCTGCTCCA15260.152437653898598No Hit
ACCTAACCTAATCAAGCTTACCCAGTGTGTTAGGCATAATACTAGCCCTC14780.14764276046011No Hit
ACCTAACCTAATCAAGCTTGTCTATGAACTGAAGGAAGAAAGATGGGACC14770.14754286684680815No Hit
ACCTAACCTAATCAAGCTTCCAACTTACAGTTCAATATCCAGAGAAAATA14070.14055031391567982No Hit
ACCTAACCTAATCAAGCTTCACTTCACTAAACAGAGCTAGTTTGATTTCT14070.14055031391567982No Hit
ACCTAACCTAATCAAGCTTTAAACCTTTTGGAATGTAAAGGCATATTTGT13940.13925169694275596No Hit
ACCTAACCTAATCAAGCTTTTAACATAGGGAGCCCCTTATTCACCATAAG13790.13775329274322848No Hit
ACCTAACCTAATCAAGCTTGGATTATCCAGGAGGGAAACCTGACAATATA13690.13675435661021013No Hit
ACCTAACCTAATCAAGCTTAGTCCTGAGACCCGGGGAGATTAAAAAAAAA13490.13475648434417345No Hit
ACCTAACCTAATCAAGCTTTTGCTTTCACTCCAATTAAAACAACAATGGT13340.13325808014464594No Hit
ACCTAACCTAATCAAGCTTAGCAAGAATTTTAAATTACTGAGTAAATGAA13130.13116031426530744No Hit
ACCTAACCTAATCAAGCTTCTGTTGAAGGCAATCCTACTGGAACTTTTAG12980.12966191006577996No Hit
ACCTAACCTAATCAAGCTTCATCTGTATTTAGCCACTCCCCATGGCTTGC12880.1286629739327616No Hit
ACCTAACCTAATCAAGCTTAGAGGACATTGTGGAGATGTTAACTGGCCTA12860.12846318670615794No Hit
ACCTAACCTAATCAAGCTTCCCCCACCTGCTATGGTGATGACATCGATCC12740.12726446334653593No Hit
ACCTAACCTAATCAAGCTTAATCAGGCAGGACATTAGAGTCCAGGGGGGC12710.12696478250663043No Hit
ACCTAACCTAATCAAGCTTGAACAACAGGGTCTTCCAACCAGGGGTTTGA12700.1268648888933286No Hit
ACCTAACCTAATCAAGCTTTATACCAGATGAAGTAAATAGAAAAGAAGAA12690.12676499528002677No Hit
ACCTAACCTAATCAAGCTTAGTCATCTGACTTAGAAAGGACAGAAACTAT12360.12346850604106627No Hit
ACCTAACCTAATCAAGCTTTGGTCTGGAACACAGTTATCTGGAAATAGTT12240.12226978268144427No Hit
ACCTAACCTAATCAAGCTTTTTGATGTGCTGCTGGATTCGGTTTGCCAGT12220.1220699954548406No Hit
ACCTAACCTAATCAAGCTTTCAGGACCCCTCACTTACACTGACACCTTCT12110.12097116570852041No Hit
ACCTAACCTAATCAAGCTTCCAGAGGAAGGATCCCTATTTTACTTTTACG12110.12097116570852041No Hit
ACCTAACCTAATCAAGCTTACCTTAAGCCTAAGAGTTATATACAATTATT12040.12027191041540759No Hit
ACCTAACCTAATCAAGCTTAGAAGGTCCCACTTTACATGTGGAGGCTCAG11950.11937286789569108No Hit
ACCTAACCTAATCAAGCTTCTGTGTTCCGAATTAGACACATCTTTATTTA11950.11937286789569108No Hit
ACCTAACCTAATCAAGCTTAGTTTGGCTGGATATGAAATTCTGAGTTGAA11880.11867361260257825No Hit
ACCTAACCTAATCAAGCTTGGGTACAGCCTGCAGCCAGTGTCCCATCTGA11840.11827403814937093No Hit
ACCTAACCTAATCAAGCTTCCTAAATCATTAAAAGGATATCCTTTTAAAG11810.11797435730946543No Hit
ACCTAACCTAATCAAGCTTACCCTAAGATCCCTATTTTACTTTTACGTGC11800.11787446369616358No Hit
ACCTAACCTAATCAAGCTTCATTATGTAGGCATGATTGATTAAATCATTA11760.11747488924295625No Hit
ACCTAACCTAATCAAGCTTTAATGGATTTTAGTCCACATTATTTTAGTAA11630.11617627227003241No Hit
ACCTAACCTAATCAAGCTTGAATCTAGGATCCCTATTTTACTTTTACGTG11520.11507744252371224No Hit
ACCTAACCTAATCAAGCTTTCAAAACAGAAAGCACCAGCCCAAATGAATT11420.1140785063906939No Hit
ACCTAACCTAATCAAGCTTTCCATATAGGGATCCCTATTTTACTTTTACG11380.11367893193748657No Hit
ACCTAACCTAATCAAGCTTGCCCAGCACCAAGCAGTCATATAGGCCACAA11330.11317946387097741No Hit
ACCTAACCTAATCAAGCTTACAGGCTTGCCTTCTGAATTTTTTCCACCTC11300.1128797830310719No Hit
ACCTAACCTAATCAAGCTTAGAGAGCACTGAACGGGATAAATGGCCAAAG11250.11238031496456273No Hit
ACCTAACCTAATCAAGCTTTCTTCATTCTTTTTTATTCTGTTTTCTCCTC11180.1116810596714499No Hit
ACCTAACCTAATCAAGCTTAACCTGTGCTTAGACATGCTTTTAAAAAGGT11170.11158116605814808No Hit
ACCTAACCTAATCAAGCTTGAGAAAGTTTCTGCATTCCCTTGTTAATTTC10810.10798499597928207No Hit
ACCTAACCTAATCAAGCTTCAGTAGGCCAGGCGCAGTGGCTCATGCCTGT10750.10738563429947105No Hit
ACCTAACCTAATCAAGCTTTCCCATCAATTCCAGTTCCCAGAACAAAAAA10750.10738563429947105No Hit
ACCTAACCTAATCAAGCTTGGAATGGCGAAGAAGAGGAAATAGCAATTGC10550.10538776203343439No Hit
ACCTAACCTAATCAAGCTTCATTAAAATTAAATCCTTTTTCTCTGGGGAA10550.10538776203343439No Hit
ACCTAACCTAATCAAGCTTTTGGATTCAACTGTAAAAACGGCAACTCCTC10460.1044887195137179No Hit
ACCTAACCTAATCAAGCTTTCAAAAGTAGGAAGCTGGTCAATGAGAATTT10420.10408914506051055No Hit
ACCTAACCTAATCAAGCTTGCCTGGTTCTTAGGCCTTCAGATCCCTATTT10360.10348978338069956No Hit
ACCTAACCTAATCAAGCTTTGGTAACTGAGCTCCTCACTGCTCCAAGTTC10290.10279052808758672No Hit
ACCTAACCTAATCAAGCTTCCACAAGCACAGGAGCCAATTCCTTAAAATA10240.10229106002107756No Hit
ACCTAACCTAATCAAGCTTTTTGATGTGTTACTGGATTTGGCATTTGGAA10230.10219116640777572No Hit
ACCTAACCTAATCAAGCTTGATTATGGGTGATGCTAGGCCCAATTTTCAC10090.10079265582155005No Hit
ACCTAACCTAATCAAGCTTAAGAGCTTCTACACAGCAAAAGAAACTATTA10050.10039308136834271No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)