FastQCFastQC Report
Mon 12 Sep 2022
EGAF00000710068

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000710068
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3787447
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTCAGGGAGTTAAGCTTCCCGTTTTTCTAGGTGAGTGTCCCAAAGTTA154545440.80463700218115No Hit
TTGTCAGGGAGTTAAGCTTCAGAACACCTTGGAATACAGTTTAGCTTCCT432271.1413229016802082No Hit
TTGTCAGGGAGTTAAGCTTGATTAGGTTAGGTATTTTTAAAGAAAACTCA424391.1205173300114826No Hit
TTGTCAGGGAGTTAAGCTTTTATCACAATGAAAGAAATACTCAAAGCATG307700.8124206094501124No Hit
TTGTCAGGGAGTTAAGCTTTCTTAGCACAGAAGGAGTAAACTGCAAATGA279040.736749583558529No Hit
TTGTCAGGGAGTTAAGCTTGTTTTCAGCCAGTGTAAGCGTGGTGTTCTTG258370.682174562442722No Hit
TTGTCAGGGAGTTAAGCTTTCCTAGGGTGCTACACAGATATATAAAGCTG254970.6731975391338809No Hit
TTGTCAGGGAGTTAAGCTTGCAAAAGGAGATCATAAGACAATTATTGGAT199820.5275849404625332No Hit
TTGTCAGGGAGTTAAGCTTGAGCTCCACCGAAACCAGCACGGAATTTCTA199710.5272945073554824No Hit
TTGTCAGGGAGTTAAGCTTGTCTATGAACTGAAGGAAGAAAGATGGGACC174610.4610229529284502No Hit
TTGTCAGGGAGTTAAGCTTGTAAAGAATTTTGTGAGCTGAAGTATTTTAA170420.4499600918507903No Hit
TTGTCAGGGAGTTAAGCTTATTTTAAAAAACGGATTATTGAAACCCAGAA163700.4322172693109633No Hit
TTGTCAGGGAGTTAAGCTTTTTTTATTTAAAAAAACCCAATAACTCAGTT119060.31435423386782707No Hit
TTGTCAGGGAGTTAAGCTTTGGAGGACAAGATGTGCTGATCATTATCTTA118930.31401099474131255No Hit
TTGTCAGGGAGTTAAGCTTAAGGATATAGCCATCCACTGTTAATTAACCT116480.30754225735700064No Hit
TTGTCAGGGAGTTAAGCTTATTTTGAGGGTAGCAACATGTCATATGTAAG104000.27459130121160774No Hit
TTGTCAGGGAGTTAAGCTTTACCTTTAAAGAGTCCGATGAACTAAAGGCA103650.27366719587099175No Hit
TTGTCAGGGAGTTAAGCTTTGAACCTAAGTTGAACCTTCCAACCCCGGCA92360.24385819788369317No Hit
TTGTCAGGGAGTTAAGCTTTGTGGAAAATGGTCTTTCAGCAGCAGCATCT90920.2400561644823017No Hit
TTGTCAGGGAGTTAAGCTTCCCGTTTTTCTAGGGGAGTGTCCCAAAGTTA77990.20591707289897393No Hit
TTGTCAGGGAGTTAAGCTTCTTGCAGCACCTTTTTTTCACTGTCGCATCA77260.20398965318854625No Hit
TTGTCAGGGAGTTAAGCTTAATTTTTCCAACAGCCCTGCTCATAAAAGGA76700.2025110846435607No Hit
TTGTCAGGGAGTTAAGCTTAATGCAGAAAATATACCCAAGTCAGGAGGTA73990.19535586900621976No Hit
TTGTCAGGGAGTTAAGCTTCCCGTTTTTCTAGGTGGGTGTCCCAAAGTTA71670.18923037074842233No Hit
TTGTCAGGGAGTTAAGCTTTATGATATTGGGCAAGTGAATAACTTCTAGC71420.18857029550512522No Hit
TTGTCAGGGAGTTAAGCTTCCCGTTTTTCTAGGTGAGTGTCCCAAGGTTA67300.17769225549558845No Hit
TTGTCAGGGAGTTAAGCTTCCCGTTTTTCTAGGCGAGTGTCCCAAAGTTA66790.1763457019992623No Hit
TTGTCAGGGAGTTAAGCTTTTTCCTTCGCAAGTACTGCTACATCTACATC66450.17544799966837818No Hit
TTGTCAGGGAGTTAAGCTTATACATAGTAGAGCAAGGAATTGAACCTAAA66400.17531598461971876No Hit
TTGTCAGGGAGTTAAGCTTGTATTGTTTCTTGGACCCATCCCCTACACTC63020.16639176733034153No Hit
TTGTCAGGGAGTTAAGCTTCCCGTTCTTCTAGGTGAGTGTCCCAAAGTTA62220.1642795265517907No Hit
TTGTCAGGGAGTTAAGCTTCTGCACTTTCAACAAAACTGTAACCAGGGGC62090.1639362874252762No Hit
TTGTCAGGGAGTTAAGCTTTAATCAGTAATCTTCAAGTAGGAAGTTATTC60640.1601078510141528No Hit
TTGTCAGGGAGTTAAGCTTCATAAAAGTATAAGCAATTCCAGATTCAAAA60340.15931576072219625No Hit
TTGTCAGGGAGTTAAGCTTCCCGTTTTTCTAGGTGAGTGCCCCAAAGTTA59760.1577843861577469No Hit
TTGTCAGGGAGTTAAGCTTCCAACTAATGAGAATGAATCTCATCAATGTC58690.15495926411643518No Hit
TTGTCAGGGAGTTAAGCTTCCCGCTTTTCTAGGTGAGTGTCCCAAAGTTA56310.14867534780024644No Hit
TTGTCAGGGAGTTAAGCTTCTAGGGAAAGACTAAATGTTCAAATTATAAT54620.1442132391555578No Hit
TTGTCAGGGAGTTAAGCTTCAGGCATCACGATATTCCCATATGTCTACTG53970.14249704352298528No Hit
TTGTCAGGGAGTTAAGCTTCCCGTTTTCCTAGGTGAGTGTCCCAAAGTTA52620.13893263720918075No Hit
TTGTCAGGGAGTTAAGCTTATCCACCAGGATCAAGTGGGCTTCATCCCTG52610.13890623419944886No Hit
TTGTCAGGGAGTTAAGCTTCCCGTTTCTCTAGGTGAGTGTCCCAAAGTTA52050.1374276656544633No Hit
TTGTCAGGGAGTTAAGCTTCCCGTTTTTCTAGGTGAGCGTCCCAAAGTTA51920.13708442652794878No Hit
TTGTCAGGGAGTTAAGCTTCTCCACAGTGAAGGAAACAATAAACAGACTA51620.1362923362359922No Hit
TTGTCAGGGAGTTAAGCTTTTAACATAGGGAGCCCCTTATTCACCATAAG49640.1310645403090789No Hit
TTGTCAGGGAGTTAAGCTTCCCGTCTTTCTAGGTGAGTGTCCCAAAGTTA49620.13101173428961513No Hit
TTGTCAGGGAGTTAAGCTTATCCCTTTTCCCTGAAAAACCTCTGTTGTAC48560.1282130152580353No Hit
TTGTCAGGGAGTTAAGCTTCACTCATTTTTTCAACCAGTTGGCTGCAATT47310.12491263904154963No Hit
TTGTCAGGGAGTTAAGCTTTAAACCTTTTGGAATGTAAAGGCATATTTGT47300.12488623603181773No Hit
TTGTCAGGGAGTTAAGCTTCCCGTTTTTCTAGGTGAGTGTCCCGAAGTTA47270.12480702700262208No Hit
TTGTCAGGGAGTTAAGCTTCCCGTTTTTCTGGGTGAGTGTCCCAAAGTTA47190.12459580292476699No Hit
TTGTCAGGGAGTTAAGCTTGGGCCTGTGATGTTCATCTCAGCCCCATGTC46490.12274759224353503No Hit
TTGTCAGGGAGTTAAGCTTTATATTATTTCTTTCAGTCCTCACAAGAGTC45900.12118981466935379No Hit
TTGTCAGGGAGTTAAGCTTTGTTGGGAGGATGCAGTGAGGTGATATATGG45660.12055614243578854No Hit
TTGTCAGGGAGTTAAGCTTAGTCCTGAGACCCGGGGAGATTAAAAAAAAA45510.12016009728981025No Hit
TTGTCAGGGAGTTAAGCTTGATGCAGTGGTTCACACCTGTAATCCCATCA44230.11678051204412894No Hit
TTGTCAGGGAGTTAAGCTTCCCGTTTTTCTAGGTGAGTGTCCCAGAGTTA43950.11604122777163614No Hit
TTGTCAGGGAGTTAAGCTTCATGTCATATGTAAGCACAATTTGGGGGGGT43790.11561877961592598No Hit
TTGTCAGGGAGTTAAGCTTCCCGTTTTTCTAGGTGAGTGTCCCAAAGCTA43790.11561877961592598No Hit
TTGTCAGGGAGTTAAGCTTACCTCATTTTGGTTTTGTCAATAAAGGGTCA42180.11136789504909243No Hit
TTGTCAGGGAGTTAAGCTTCATAAGTGAAGGAGAAATAAAATCCTTTACA42170.11134149203936056No Hit
TTGTCAGGGAGTTAAGCTTTCAAACAGAATTTAAAGAGTTAACGCTGGCC41680.11004774456249816No Hit
TTGTCAGGGAGTTAAGCTTCTGGTGTACTGAATCTTTTCCATTTAACAAA41030.10833154892992561No Hit
TTGTCAGGGAGTTAAGCTTTTTAGCTGAAGGAGGCTTTGAGCCAAATTTA40800.10772427970609226No Hit
TTGTCAGGGAGTTAAGCTTTGAGGTCTGCACATCAGATAGCATTACTCCC40680.10740744358930963No Hit
TTGTCAGGGAGTTAAGCTTTAGAATTGTAGTTCCAATGCTATCAAGGAAG40510.10695859242386759No Hit
TTGTCAGGGAGTTAAGCTTTTTTTCTTGTCTTTTTGTATGTTTCATAATA39890.10532160582049069No Hit
TTGTCAGGGAGTTAAGCTTTACAGTGGCTCCATTTCACTCAGGCTCACCA39230.10357900717818626No Hit
TTGTCAGGGAGTTAAGCTTTGATTTTTGCAGCTCCCCTGAAGATTTGGAA38860.1026020958181065No Hit
TTGTCAGGGAGTTAAGCTTTAGTTGGCAAGGCAGTAGCAATTCTGGGTGC38790.10241727474998329No Hit
TTGTCAGGGAGTTAAGCTTACTAAAGGTCAGAAAATTATCCATTTTACCC38690.10215324465266445No Hit
TTGTCAGGGAGTTAAGCTTCTTTTAAGAAATCAGACTGTTTATACCCCAA38680.10212684164293256No Hit
TTGTCAGGGAGTTAAGCTTTTCTGCCAGACTACTCAGAAGTAAAGTGATT38450.10151957241909919No Hit
TTGTCAGGGAGTTAAGCTTAGAGGACATTGTGGAGATGTTAACTGGCCTA38340.10122913931204845No Hit
TTGTCAGGGAGTTAAGCTTTACCTAAAACCTTGAGATATTGTTAAATAAA37910.10009380989357738No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)