FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000710378

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000710378
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1539545
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGACCTTTAGTAAGCTTTCACTAGAGCAAAGGAAGTCCCTCAACATAA23729215.413125306502895No Hit
TCTGACCTTTAGTAAGCTTGCATGTCTGCCTTCTTCCTCATAGAAACACT168621.0952586640858175No Hit
TCTGACCTTTAGTAAGCTTTTTCAACCAGAGAAACAGTATGCTACAAGAG55550.36082089188688865No Hit
TCTGACCTTTAGTAAGCTTCCAACTAATGAGAATGAATCTCATCAATGTC52360.3401004842339782No Hit
TCTGACCTTTAGTAAGCTTGCCACTGCAGACTGGTGTTCAGAGAAAGCCA51370.3336700128934198No Hit
TCTGACCTTTAGTAAGCTTTTAGTTGGAAAGATTGTTCAATGATCTAATT48620.3158075925029798No Hit
TCTGACCTTTAGTAAGCTTAGAAAGTTTAAGAAACTGGCTTAAGATCTAA48390.3143136446157793No Hit
TCTGACCTTTAGTAAGCTTAGTCCTGAGACCCGGGGAGATTAAAAAAAAA47380.30775326476329046No Hit
TCTGACCTTTAGTAAGCTTCCCTCTACAACATCCTCTCTTTCCAGCTGTG47280.30710372220363813No Hit
TCTGACCTTTAGTAAGCTTCCCGTTTTTCTAGGTGAGTGTCCCAAAGTTA47040.3055448200604724No Hit
TCTGACCTTTAGTAAGCTTAGTTTGGCTGGATATGAAATTCTGGGTTGAA46520.3021671987502801No Hit
TCTGACCTTTAGTAAGCTTTCTGGATTAAAAAATATATATATGCCACCAA46460.30177747321448867No Hit
TCTGACCTTTAGTAAGCTTGGAATGGCGAAGAAGAGGAAATAGCAATTGC46210.3001536168153578No Hit
TCTGACCTTTAGTAAGCTTCTATTATAAACATTGTGACTTTGTGCAATTT45650.2965161784813045No Hit
TCTGACCTTTAGTAAGCTTGCCCAGCACCAAGCAGTCATATAGGCCACAA45330.29443764229041697No Hit
TCTGACCTTTAGTAAGCTTACTGTCCCATGAATTATCATAATATGACAAA44950.291969380563738No Hit
TCTGACCTTTAGTAAGCTTGATCTAATTTAAAAATTTAAAGAAGGATTAT43480.2824211049368482No Hit
TCTGACCTTTAGTAAGCTTCTGAAAGATCTAATTAAAAAATTTAAAGAAG43060.2796930261863083No Hit
TCTGACCTTTAGTAAGCTTCTTGCAGCACCTTTTTTTCACTGTCGCATCA43050.27962807193034306No Hit
TCTGACCTTTAGTAAGCTTGGGCTTGTAAAACTTGAGCTGCAAATTTTTA42000.27280787505399323No Hit
TCTGACCTTTAGTAAGCTTAACTCCCTGACAAAACTAGGAATTCCCTGAA41890.27209337823837565No Hit
TCTGACCTTTAGTAAGCTTCTATAGATCTAATTTAAAAATTTAAAGAAGG41510.2696251165116966No Hit
TCTGACCTTTAGTAAGCTTTCCTAGGGTGCTACACAGATATATAAAGCTG41300.26826107713642666No Hit
TCTGACCTTTAGTAAGCTTATCCACCATGATCTAATTTAAAAATTTAAAG39300.2552702259433794No Hit
TCTGACCTTTAGTAAGCTTAATTAACAAGGAGTAACTTGCCCAAGATCTA38290.24870984609089047No Hit
TCTGACCTTTAGTAAGCTTTTCTGCTTCTTAGGGGGCTTTAATCTAAAGA36590.23766762257680027No Hit
TCTGACCTTTAGTAAGCTTTTAAAGTATATTAGAGTTGATTGGGTTAGAC36450.23675826299328698No Hit
TCTGACCTTTAGTAAGCTTTAGTGTGCTCAGAATGACCAGAGAACCTTGT36200.23513440659415608No Hit
TCTGACCTTTAGTAAGCTTCAAAGAAAGTTGGGGTTAGTTTTTTTGCCAA36030.23403018424274705No Hit
TCTGACCTTTAGTAAGCTTCTGTCCGAAGAGAGCAGTGTGCTCCTCCTCC35930.2333806416830947No Hit
TCTGACCTTTAGTAAGCTTCTGTGTTCCGAATTAGACACATCTTTATTTA35270.22909366078938906No Hit
TCTGACCTTTAGTAAGCTTCAAATGACGCTGATGCCCTGGTCTGCCACCC35180.22850907248570196No Hit
TCTGACCTTTAGTAAGCTTCCTGAAGAGTCATCAGCAGCTGGAGACCATG34190.22207860114514355No Hit
TCTGACCTTTAGTAAGCTTGCAAAAGGAGATCTAATTTAAAAATTTAAAG33260.21603785534037653No Hit
TCTGACCTTTAGTAAGCTTAGGCAAATGATGAGACAATATTTCATCAAAA32350.21012701804754003No Hit
TCTGACCTTTAGTAAGCTTGATCTAATTAAAAAATTTAAAGAAGGATTAT32160.20889288718420052No Hit
TCTGACCTTTAGTAAGCTTATCCACCACGATCTAATTTAAAAATTTAAAG31810.20661948822541723No Hit
TCTGACCTTTAGTAAGCTTGCATGTGTTTGAAAAAGATCTAATTTAAAAA31020.20148810200416356No Hit
TCTGACCTTTAGTAAGCTTCCTGAAGATGATGTCTGGTTTGGTTTGTTGT30880.20057874242065027No Hit
TCTGACCTTTAGTAAGCTTCTGTTGAAGGCAATCCTACTGGAACTTTTAG30500.19811048069397125No Hit
TCTGACCTTTAGTAAGCTTGGTGGACCTCCAGAACCATTTGAGGGTACTT30250.19648662429484037No Hit
TCTGACCTTTAGTAAGCTTCCTTTTCAAGTCTCAAGTACATATTTAAAGC30100.1955123104553618No Hit
TCTGACCTTTAGTAAGCTTGCCCAAGGATGATCTAATTTAAAAATTTAAA29990.19479781363974422No Hit
TCTGACCTTTAGTAAGCTTCATAGAAACCTAAAGGAGTTAGAGAAGGAAG29910.19427817959202232No Hit
TCTGACCTTTAGTAAGCTTAATAATCAACATAGAAGTGTTATGAGCTTTA29900.1942132253360571No Hit
TCTGACCTTTAGTAAGCTTACCTTAAGCCTAAGAGTTATATACAATTATT29880.1940833168241266No Hit
TCTGACCTTTAGTAAGCTTTCCCATCAATTCCAGTTCCCAGAACAAAAAA29720.19304404872868283No Hit
TCTGACCTTTAGTAAGCTTCATTATGTAGGCATGATTGATTAAATCATTA29590.19219964340113474No Hit
TCTGACCTTTAGTAAGCTTTTTTTATTTAAAAAAACCCAATAACTCAGTT29200.18966642741849052No Hit
TCTGACCTTTAGTAAGCTTGCAAAAGGAGATCTAATTAAAAAATTTAAAG28890.1876528454835682No Hit
TCTGACCTTTAGTAAGCTTTGGAGGACAAGATGTGCTGATCTAATTTAAA28860.1874579827156725No Hit
TCTGACCTTTAGTAAGCTTCCAATTGGAACTCAGAATACAAAGGAAATGG28370.18427522417337588No Hit
TCTGACCTTTAGTAAGCTTCTGAAAGATCTAATTTAAAAATTTAAAGAAG28200.18317100182196688No Hit
TCTGACCTTTAGTAAGCTTTTTCTATATTTTGGATACAAGTCCTTCGTCA28050.18219668798248834No Hit
TCTGACCTTTAGTAAGCTTAGCAAGAATTTTAAATTACTGAGTAAATGAA27850.1808976028631836No Hit
TCTGACCTTTAGTAAGCTTAGAAAAGAGAACCTAGGAATGAAGACACCAA27710.17998824327967028No Hit
TCTGACCTTTAGTAAGCTTAGGTTTATAATCAGATAAATCCAATTCATTA27110.17609098792175612No Hit
TCTGACCTTTAGTAAGCTTGATTAGGTTAGGTATTTTTAAAGAAAACTCA26990.17531153685017326No Hit
TCTGACCTTTAGTAAGCTTTCATAGCCTTTAAAATGTCTCTATTCTGTAT26910.17479190280245138No Hit
TCTGACCTTTAGTAAGCTTTAAACCTTTTGGAATGTAAAGGCATATTTGT26800.17407740598683377No Hit
TCTGACCTTTAGTAAGCTTCCTTGTGTTTCAAAGTATATCAACCCTAACA26080.16940069955733675No Hit
TCTGACCTTTAGTAAGCTTAATGCAGAAAATATACCCAAGTCAGGAGGTA25760.1673221633664492No Hit
TCTGACCTTTAGTAAGCTTGAACAACAGGGTCTTCCAACCAGGGGTTTGA25610.16634784952697063No Hit
TCTGACCTTTAGTAAGCTTGAGGCCTGTCTTTTTTCCTTCTTCTTTTTTT25170.1634898622645002No Hit
TCTGACCTTTAGTAAGCTTGATTATGGGTGATGCTAGGCCCAATTTTCAC25150.16335995375256976No Hit
TCTGACCTTTAGTAAGCTTTCAAACAGAATTTAAAGAGTTAACGCTGGCC24670.1602421494662384No Hit
TCTGACCTTTAGTAAGCTTTTCAAATATGTAAGATCTAATTTAAAAATTT24640.1600472866983427No Hit
TCTGACCTTTAGTAAGCTTATTTTTGACATATGACAACCATATTTTGGAT24600.15978746967448174No Hit
TCTGACCTTTAGTAAGCTTCTCTGTTCCCTGATGCAGTGTGGATATTTTT24440.15874820157903796No Hit
TCTGACCTTTAGTAAGCTTCTACCACCTCTTCTGGAATGGTTCTAGCTAG24240.15744911645973325No Hit
TCTGACCTTTAGTAAGCTTCCAACTTACAGTTCAATATCCAGAGAAAATA24210.15725425369183754No Hit
TCTGACCTTTAGTAAGCTTGTTGTCATCAAATAAAACGCACAGCAAAGCT23980.1557603058046371No Hit
TCTGACCTTTAGTAAGCTTTGATATTCTTGTGTTTTCTAGGATTTTCTAA23930.1554355345248109No Hit
TCTGACCTTTAGTAAGCTTTATTAAGTCATAGTTTCTCTTTCAATGGTAC23890.15517571750094994No Hit
TCTGACCTTTAGTAAGCTTTGCATTGTTTACCTTGCTGAGTGCCTGCTGC23510.15270745577427097No Hit
TCTGACCTTTAGTAAGCTTTGAAACATTAAGTATAGTCTTTATTCATGTT23430.15218782172654907No Hit
TCTGACCTTTAGTAAGCTTTGAAAAAGGTCAAGGTTAGTTAGTGTCTACA23420.15212286747058384No Hit
TCTGACCTTTAGTAAGCTTTAATTCTCACAGAAGAACTGTGATAGTTTTT23230.15088873660724433No Hit
TCTGACCTTTAGTAAGCTTCCAGGATAAGCCTCGGGGATCTAATTAAAAA22170.14400358547492928No Hit
TCTGACCTTTAGTAAGCTTCTATGTAGCAACTAGTTCATTTCTCCATTCG22100.14354890568317263No Hit
TCTGACCTTTAGTAAGCTTTCTATGACTCACCAAGGCTGGGTTGGGTGCT22060.1432890886593117No Hit
TCTGACCTTTAGTAAGCTTCCAGCACCAGACAGGGAAGAAGGAGTTCTGA22010.1429643173794855No Hit
TCTGACCTTTAGTAAGCTTGCAAAATTTTCAGCCTGACCACGTGGTAGAA21790.14153532374825029No Hit
TCTGACCTTTAGTAAGCTTATTGCAGACATTTCCATCCATTTTAGAGTTC21650.14062596416473697No Hit
TCTGACCTTTAGTAAGCTTACCCAGTGTGTTAGGCATAATACTAGCCCTC21590.14023623862894555No Hit
TCTGACCTTTAGTAAGCTTTCTAGTCTTTCTCAAGGAAACCTGTCCAAAG21500.13965165032525845No Hit
TCTGACCTTTAGTAAGCTTAAATTTAAGACAATTTCTGATAAACCTGAAA21460.13939183330139748No Hit
TCTGACCTTTAGTAAGCTTTAGAATTGTAGTTCCAATGCTATCAAGGAAG21330.13854742797384942No Hit
TCTGACCTTTAGTAAGCTTTCTGCAGCCCACGCTGTACAGAAATAATTAA21290.13828761094998848No Hit
TCTGACCTTTAGTAAGCTTAAGGAGGGGAGATGAAGAGCAAGTGATTATG21130.13724834285454468No Hit
TCTGACCTTTAGTAAGCTTGAACAAAGCACAGTGGATATTGAAAGAAAGA20880.13562448645541378No Hit
TCTGACCTTTAGTAAGCTTATCCACCATGATCTAATTAAAAAATTTAAAG20710.13452026410400475No Hit
TCTGACCTTTAGTAAGCTTTTATGCTCTGCTTCCCTTTTAAATATAAGTT20530.1333510874966305No Hit
TCTGACCTTTAGTAAGCTTTACAATACCCTGATATATAGGAATTATACTA20320.13198704812136053No Hit
TCTGACCTTTAGTAAGCTTAGATTTGATATTTGAGAAAATGGGAATCTTT20300.13185713960943005No Hit
TCTGACCTTTAGTAAGCTTTCCCCAGGCCAAGGCACCAGCTGCTGCTCCA20250.13153236832960388No Hit
TCTGACCTTTAGTAAGCTTTCACAAGTACTAAAAATTTTTTTCTATCTTC20210.13127255130574292No Hit
TCTGACCTTTAGTAAGCTTGTAAAGAATTTTGTGAGCTGAAGTATTTTAA20120.1306879630020558No Hit
TCTGACCTTTAGTAAGCTTTATGAAAAAGAGGCCAGAGGAGGGAGACTGG20050.13023328321029914No Hit
TCTGACCTTTAGTAAGCTTCTAGGGAGCAGAGATGATGGTGTAATTCCTT19970.12971364916257727No Hit
TCTGACCTTTAGTAAGCTTCTGCGTCAGCAATTCACAATCAATTTGCTTT19740.12821970127537682No Hit
TCTGACCTTTAGTAAGCTTGGTGCTGGTGAATCAATGCTCTGTGGATTGA19660.12770006722765492No Hit
TCTGACCTTTAGTAAGCTTGGGTACAGCCTGCAGCCAGTGTCCCATCTGA19630.1275052044597592No Hit
TCTGACCTTTAGTAAGCTTAAGAGCAGTGGGTGCAATTATCCCAAATATC19610.12737529594782873No Hit
TCTGACCTTTAGTAAGCTTGCTTGAGGCTCATAAATCTTGCATTATTTGG19610.12737529594782873No Hit
TCTGACCTTTAGTAAGCTTTGGAGGACAAGATGTGCTGATCTAATTAAAA19560.12705052466800257No Hit
TCTGACCTTTAGTAAGCTTTTCCCTCAGAATTCTGATTTTTATTCTGAAA19500.12666079913221112No Hit
TCTGACCTTTAGTAAGCTTTCTCTTGATGTCCAATCACAGAATAACAGTA19500.12666079913221112No Hit
TCTGACCTTTAGTAAGCTTACAGGCTTGCCTTCTGAATTTTTTCCACCTC19430.12620611934045448No Hit
TCTGACCTTTAGTAAGCTTTTGCTTTCACTCCAATTAAAACAACAATGGT19290.12529675975694118No Hit
TCTGACCTTTAGTAAGCTTTCAGGACCCCTCACTTACACTGACACCTTCT19170.12451730868535833No Hit
TCTGACCTTTAGTAAGCTTCCAAATCAGGTGACTTTCAGTTAATAAAAGA19030.12360794910184503No Hit
TCTGACCTTTAGTAAGCTTCCTAAATCATTAAAAGGATATCCTTTTAAAG18800.12211400121464458No Hit
TCTGACCTTTAGTAAGCTTCAAATGGGAAGGATCTAATTAAAAAATTTAA18590.12074996183937463No Hit
TCTGACCTTTAGTAAGCTTTGGGATTCATTGAAGAGCTACACCCCAGAAA18570.12062005332744415No Hit
TCTGACCTTTAGTAAGCTTGGCAAAATTTCTCAGCACTAAGACTTCCTCT18470.11997051076779179No Hit
TCTGACCTTTAGTAAGCTTCTGTTCTGAATTTTCCAGACCCATAATTTTG18320.11899619692831323No Hit
TCTGACCTTTAGTAAGCTTATTTTGAGGGTAGCAACATGGCGTTCCCAAC18310.118931242672348No Hit
TCTGACCTTTAGTAAGCTTTTCTTTCAAGGGTGAATAATATTAATACTTG18240.11847656288059136No Hit
TCTGACCTTTAGTAAGCTTATAAAAATTTAAATAGTAATACAAGACAGAA18190.11815179160076517No Hit
TCTGACCTTTAGTAAGCTTTCCATATAGGGATCTAATTAAAAAATTTAAA18130.11776206606497375No Hit
TCTGACCTTTAGTAAGCTTCTGGATAAATCTGACAGCAATGTCTTACATG18110.11763215755304327No Hit
TCTGACCTTTAGTAAGCTTTATAAAAATTTTTTTTGCCAAAACATACATA18020.11704756924935614No Hit
TCTGACCTTTAGTAAGCTTTGAACCTAAGTTGAACCTTCCAACCCCGGCA18010.11698261499339091No Hit
TCTGACCTTTAGTAAGCTTATACATAGTAGAGCAAGGAATTGAACCTAAA17990.11685270648146044No Hit
TCTGACCTTTAGTAAGCTTCACGGTGCAGTACATTTCCATATGGACAGGG17940.11652793520163425No Hit
TCTGACCTTTAGTAAGCTTAGTGAGGAAGGCATGTATAATGCTGAAAGCT17930.11646298094566902No Hit
TCTGACCTTTAGTAAGCTTGCCCAAGGATGATCTAATTAAAAAATTTAAA17840.1158783926419819No Hit
TCTGACCTTTAGTAAGCTTTCAAAACAGAAAGCACCAGCCCAAATGAATT17800.11561857561812093No Hit
TCTGACCTTTAGTAAGCTTTTCAAATATGTAAGATCTAATTAAAAAATTT17750.11529380433829475No Hit
TCTGACCTTTAGTAAGCTTTATAGCAGCCAACAAGGACAGGAAAATATAA17740.11522885008232951No Hit
TCTGACCTTTAGTAAGCTTTCTCCTCAAACTGGGAAGCAAGAGACCCCAG17610.11438444475478146No Hit
TCTGACCTTTAGTAAGCTTTTAGATGTCTCTGTTTACTTTGATCTAATTT17580.11418958198688575No Hit
TCTGACCTTTAGTAAGCTTCATTATACATATGCACGTGCATGCATGCTAA17530.11386481070705956No Hit
TCTGACCTTTAGTAAGCTTCCATAAAAACCTAAAATGACTGGGTTCAAGA17500.11366994793916385No Hit
TCTGACCTTTAGTAAGCTTTGATTTTTGCAGCTCCCCTGAAGATTTGGAA17470.11347508517126814No Hit
TCTGACCTTTAGTAAGCTTCTGGGATTATTTTTTGATTGTTGATTGTGAT17420.11315031389144195No Hit
TCTGACCTTTAGTAAGCTTCATGAGGTCAGGACATATCTCCTTTTTGTTT17350.1126956340996853No Hit
TCTGACCTTTAGTAAGCTTCTCGCACTTTTTCATGCGATTTTCATGGTTC17250.11204609154003294No Hit
TCTGACCTTTAGTAAGCTTGGTGGAGTTATTTAGCAGCCTGCTCACAGAT17140.11133159472441533No Hit
TCTGACCTTTAGTAAGCTTATGGACATTATGCTCCTGCATAACATTGATT17110.11113673195651962No Hit
TCTGACCTTTAGTAAGCTTCAATATCTGAGGGCAGGAGAAGGTAGATATC17070.11087691493265868No Hit
TCTGACCTTTAGTAAGCTTACCAAATTTAAGGCACCGTGCTAAAAGTTGG16970.11022737237300631No Hit
TCTGACCTTTAGTAAGCTTAGAGGACATTGTGGAGATGTTAACTGGCCTA16920.10990260109318013No Hit
TCTGACCTTTAGTAAGCTTATCAAGGAATCTCAACCCATGCAGTACTCTT16600.10782406490229256No Hit
TCTGACCTTTAGTAAGCTTCTGATGTAAAATTAGTCATCAATTTAGCTAT16590.10775911064632732No Hit
TCTGACCTTTAGTAAGCTTATCCACCAGGATCTAATTTAAAAATTTAAAG16510.10723947659860543No Hit
TCTGACCTTTAGTAAGCTTCCAAGAGGCAAAACAATCATCAGAACCAGAC16450.10684975106281401No Hit
TCTGACCTTTAGTAAGCTTTGTACCACTGCCTTGATCTAATTAAAAAATT16430.10671984255088353No Hit
TCTGACCTTTAGTAAGCTTTATTCCACTGCTGTCAAAGTGATGACACACC16320.10600534573526595No Hit
TCTGACCTTTAGTAAGCTTCCTCTAAGTATGAAATTTATGTATTTGGGCT16260.10561562019947453No Hit
TCTGACCTTTAGTAAGCTTAGCTTATTAATTATTTCTTTCATGGATCTAA16180.10509598615175264No Hit
TCTGACCTTTAGTAAGCTTTTAGATGTCTCTGTTTACTTTGATCTAATTA16120.10470626061596122No Hit
TCTGACCTTTAGTAAGCTTGCCTTTGTCAGCTGATCTAATTTAAAAATTT15990.10386185528841313No Hit
TCTGACCTTTAGTAAGCTTGCAATTTATCAGATCTAATTAAAAAATTTAA15980.1037969010324479No Hit
TCTGACCTTTAGTAAGCTTTTCCATCTTCTCAACATTGCCTTCACTTGCA15940.10353708400858694No Hit
TCTGACCTTTAGTAAGCTTTGCAGAAGAATTCACAATAGTTTTCCTATAA15840.10288754144893458No Hit
TCTGACCTTTAGTAAGCTTTGAGGTCTGCACATCAGATAGCATTACTCCC15780.10249781591314318No Hit
TCTGACCTTTAGTAAGCTTTTAGCCTTCAAGAAATGACATTTTGCTTTCA15750.10230295314524747No Hit
TCTGACCTTTAGTAAGCTTCATAAGTGAAGGAGAAATAAAATCCTTTACA15720.10210809037735176No Hit
TCTGACCTTTAGTAAGCTTACTAAATTTTTCAGTTAATGATGGTGGCATT15650.10165341058559509No Hit
TCTGACCTTTAGTAAGCTTCCTCCTTGGCCCACATTCCCTAACTTTGCAC15630.10152350207366463No Hit
TCTGACCTTTAGTAAGCTTTGCTAGAAGCTCTGATCTAATTAAAAAATTT15610.10139359356173415No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)