FastQCFastQC Report
Mon 12 Sep 2022
EGAF00000710379

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000710379
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5926823
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACAGCCACACCAGCAAGCTTATCTCAGATGATTAAATGGCAGCAGATA104846117.69010142533361No Hit
GAACAGCCACACCAGCAAGCTTAATGATACGGCGCCCAGCAAAATGTCGT2881064.861052877739052No Hit
GAACAGCCACACCAGCAAGCTTGATACGGCGCCCAGCAAAATGTCGTATG1998813.3724813445584587No Hit
GAACAGCCACACCAGCAAGCTTAATGAGACGGCGCCCAGCAAAATGTCGT1381802.3314345645213295No Hit
GAACAGCCACACCAGCAAGCTTATGATACGGCGCCCAGCAAAATGTCGTA1270292.1432899210926326No Hit
GAACAGCCACACCAGCAAGCTTGAGACGGCGCCCAGCAAAATGTCGTATG1129421.9056077767127515No Hit
GAACAGCCACACCAGCAAGCTTATGAGACGGCGCCCAGCAAAATGTCGTA803671.3559878538636974No Hit
GAACAGCCACACCAGCAAGCTTATACGGCGCCCAGCAAAATGTCGTATGC792261.3367363931738807No Hit
GAACAGCCACACCAGCAAGCTTGCTTTGAACTCCAAGTACCTCCAAGGTC666321.1242448104152933No Hit
GAACAGCCACACCAGCAAGCTTTGTCATTTTGCTCTTAACAATAAACCTT601991.0157043664033834No Hit
GAACAGCCACACCAGCAAGCTTAGACGGCGCCCAGCAAAATGTCGTATGC542350.9150770994848336No Hit
GAACAGCCACACCAGCAAGCTTCCATGTGATCTGCCTGCCTCAGCCTCCC485650.8194103316397335No Hit
GAACAGCCACACCAGCAAGCTTAGCTCAGTTAACTCAGCACACAGTTCGT462380.7801481501978379No Hit
GAACAGCCACACCAGCAAGCTTCGCTTCTGTGAGCCCCCTTGTCTGTTGG419120.7071579495456504No Hit
GAACAGCCACACCAGCAAGCTTACGGCGCCCAGCAAAATGTCGTATGCCG395100.6666303346666502No Hit
GAACAGCCACACCAGCAAGCTTCTCCCTGGGCAGGCTGTGTTGCTAACAC382830.6459278436356206No Hit
GAACAGCCACACCAGCAAGCTTACTGAATCAAGATCTTGCTTGGTGGGGT284510.4800379562541348No Hit
GAACAGCCACACCAGCAAGCTTTGCCTGGCTGACAAAGGGAGGAGGGGCA269760.4551510986577463No Hit
GAACAGCCACACCAGCAAGCTTCATGTGATCTGCCTGCCTCAGCCTCCCA256050.4320189754274761No Hit
GAACAGCCACACCAGCAAGCTTATGCTTAAAACTATTTAAATTCAAAAGT247780.4180654627276704No Hit
GAACAGCCACACCAGCAAGCTTCAACTACATCCTAATTACCCAGTGGGAA214450.3618296007827465No Hit
GAACAGCCACACCAGCAAGCTTTACGGCGCCCAGCAAAATGTCGTATGCC179900.30353530044679927No Hit
GAACAGCCACACCAGCAAGCTTTAGAGCTGAGCCTTGGAGGAGGGGTGTC172340.2907797314007859No Hit
GAACAGCCACACCAGCAAGCTTATGACATAAAGGCTTTATTTATTTATTT170210.2871859004394091No Hit
GAACAGCCACACCAGCAAGCTTGTCCGCATGTGATCTGCCTGCCTCAGCC166970.28171922799111765No Hit
GAACAGCCACACCAGCAAGCTTATGAAGTGTTACAAGATAAGGGCAACAG161850.27308053572715096No Hit
GAACAGCCACACCAGCAAGCTTTGATACGGCGCCCAGCAAAATGTCGTAT157970.2665340267458637No Hit
GAACAGCCACACCAGCAAGCTTGATTCAAAAATCCAACCACATGTGATCT147500.24886857596388487No Hit
GAACAGCCACACCAGCAAGCTTGGCCAGCCTTGGCTGTTTTCTGATCCCG143350.24186651094523998No Hit
GAACAGCCACACCAGCAAGCTTCGTCCAGGGTTCTTTATTTTTGTCTGCT140950.23781712394650556No Hit
GAACAGCCACACCAGCAAGCTTCTGGAGTGTTTGCCCAGTCTCCGGGGAC138260.23327843601875742No Hit
GAACAGCCACACCAGCAAGCTTGTCTTTTATTTTGTTACACATGTGATCT136480.2302751406613628No Hit
GAACAGCCACACCAGCAAGCTTATCATAGCATCTTAAAGTTGGAAGGGAC134100.2262594985542845No Hit
GAACAGCCACACCAGCAAGCTTGTGCAATACATGTGATCTGCCTGCCTCA133120.2246059988631346No Hit
GAACAGCCACACCAGCAAGCTTTTACCTGGTGAATTTAAGAAGACATAAT132800.22406608059663669No Hit
GAACAGCCACACCAGCAAGCTTCACATGTGATCTGCCTGCCTCAGCCTCC126470.2133858223874747No Hit
GAACAGCCACACCAGCAAGCTTGATCAGGAGAATATTGAAGGGAGACTCG122360.20645124715214205No Hit
GAACAGCCACACCAGCAAGCTTCGCTTCTATAATGATGATGAGCTAGTCT121880.20564136975239516No Hit
GAACAGCCACACCAGCAAGCTTCTTGGCCCCAGGACTGCCCCAGGGGTGG119070.2009002124747103No Hit
GAACAGCCACACCAGCAAGCTTGGTTTCCTCACCTTGAAAGTGGGAATTA118170.1993816923501849No Hit
GAACAGCCACACCAGCAAGCTTTTCTTTAGTAAGACTAATGCTATCAAGG117370.1980318966839401No Hit
GAACAGCCACACCAGCAAGCTTCCCAGAATGGTTGCTGTCTCTGAAGGAT111880.18876892392433517No Hit
GAACAGCCACACCAGCAAGCTTTGTGCTTGGGTTTGGGCACTGCAGCTGT110340.18617056726681394No Hit
GAACAGCCACACCAGCAAGCTTCGTGTCCATTTACAGTCACTTTCCATTG107090.18068702237269443No Hit
GAACAGCCACACCAGCAAGCTTCAGGAAAGAATAGGCTGCACAGGGGCGC103390.17444421741631225No Hit
GAACAGCCACACCAGCAAGCTTTGTTCTCTTCATCTGGCCTCAGAGTTAG102780.1734149982208006No Hit
GAACAGCCACACCAGCAAGCTTTATCCTACTGTAATAGGCTGCTCCAAAG101950.1720145852170716No Hit
GAACAGCCACACCAGCAAGCTTTGAGACGGCGCCCAGCAAAATGTCGTAT98750.16661540255209242No Hit
GAACAGCCACACCAGCAAGCTTGACCACTTCTAGCCAGGGCCTCGGTGGC98420.16605861183976642No Hit
GAACAGCCACACCAGCAAGCTTCCAACCCCCTCCCCTCAGTCTCACACCC98210.16570429047737717No Hit
GAACAGCCACACCAGCAAGCTTTACCCTGGATGGAGAGTGTATCTCATTT95190.16060881183730305No Hit
GAACAGCCACACCAGCAAGCTTAATTAATGCTTACAAAGTGCTCTGAGGC94120.15880346013370064No Hit
GAACAGCCACACCAGCAAGCTTCTGCCCTATTTCTGGCCCCACGTCCCAA93890.15841539387965525No Hit
GAACAGCCACACCAGCAAGCTTCAGTGGCCTGCTCCATCCCCTAATGACT92390.15588452700544625No Hit
GAACAGCCACACCAGCAAGCTTCTAGAAAATTCCAACTAAGAAACTGGAG91850.15497341493073102No Hit
GAACAGCCACACCAGCAAGCTTTTCCATTTTTTCTTGCACAAAGCAGCTG91750.15480469047245043No Hit
GAACAGCCACACCAGCAAGCTTTGGGTATATGACAGGAGGCAGCGTTTGA91260.15397794062687548No Hit
GAACAGCCACACCAGCAAGCTTATCCACCATGTGATCTGCCTGCCTCAGC88990.15014789542390586No Hit
GAACAGCCACACCAGCAAGCTTATAACAAAAGATATAACAGAGAGTTTAT88440.14921991090336256No Hit
GAACAGCCACACCAGCAAGCTTTTCATGTGATCTGCCTGCCTCAGCCTCC86230.1454911003753613No Hit
GAACAGCCACACCAGCAAGCTTATCTCAGATTATTAAATGGCAGCAGATA83600.14105364712258153No Hit
GAACAGCCACACCAGCAAGCTTAAACAAGCCCTGAAAGGATCAATCTGAA82730.1395857443355403No Hit
GAACAGCCACACCAGCAAGCTTCCAGGGCCCGACTGCCCAGCACAGCCCA81620.13771290284862564No Hit
GAACAGCCACACCAGCAAGCTTCTTGACATGTGATCTGCCTGCCTCAGCC78770.13290425578762854No Hit
GAACAGCCACACCAGCAAGCTTGTACCCACGCTGTGCCACTCAGAAGGAA78050.13168943968800823No Hit
GAACAGCCACACCAGCAAGCTTCATAAGTGAAGGAGAAATAAAATACTTT76630.1292935523804237No Hit
GAACAGCCACACCAGCAAGCTTGACGGCGCCCAGCAAAATGTCGTATGCC73920.12472111956101944No Hit
GAACAGCCACACCAGCAAGCTTCAAGATGGTATCTTTAGGATAATTACAT73500.12401247683624093No Hit
GAACAGCCACACCAGCAAGCTTACATCATTACCAAAGAAAAAAGTAAAAT73440.12391124216127257No Hit
GAACAGCCACACCAGCAAGCTTGAGAAGGGAGGCTGGTCTTGGGAGAGGC72570.12244333937423135No Hit
GAACAGCCACACCAGCAAGCTTAATGATACGGCGACCAGCAAAATGTCGT72490.12230835980760688No Hit
GAACAGCCACACCAGCAAGCTTGCATTCTAGTCCGAGGAGCAGACAGAAT71340.12036802853737999No Hit
GAACAGCCACACCAGCAAGCTTTTCATTCCTGGATCAGAGAGGACTGGCA71050.11987872760836624No Hit
GAACAGCCACACCAGCAAGCTTCCCAGTCCCTGGATTGGATTCCCACATT68660.11584621305545989No Hit
GAACAGCCACACCAGCAAGCTTCAGTCTCCCAAAGTGCTGGGATTACAGG66790.11269106568561268No Hit
GAACAGCCACACCAGCAAGCTTGGCACTATTCCAGACCCCGTGGGGCACT65490.11049764772796489No Hit
GAACAGCCACACCAGCAAGCTTCCTGAGAGCAGAAAGTTTTGCTACTTTT64830.10938406630331293No Hit
GAACAGCCACACCAGCAAGCTTTGAATCCTGAACTCCCAGCCCCAGCATT64230.10837171955362931No Hit
GAACAGCCACACCAGCAAGCTTAAGCAATGTGGCCTCCCAAAATGCTGGG62630.10567212822113974No Hit
GAACAGCCACACCAGCAAGCTTTAACCGCTGTCCCTCATGTGATCTGCCT61350.10351245515514804No Hit
GAACAGCCACACCAGCAAGCTTGTCTTTATTGATTTTACATTCAGCACAA61340.10349558270931998No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)