FastQCFastQC Report
Sun 11 Sep 2022
EGAF00000710381

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000710381
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3342277
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGGTTCTCGCAAGCTTCAGGAATACAAAGCTAAAAGACAAGGTATCCC158332347.37258461821088No Hit
CGAGGTTCTCGCAAGCTTCACAGTCTGGGGAGGTGAAAGGGAAGCAAACC401211.2004091821234446No Hit
CGAGGTTCTCGCAAGCTTACAAATTTCTAGGCAAAAAGCACACTTCAGGA373131.1163946016443282No Hit
CGAGGTTCTCGCAAGCTTTCCTTTTATCACCTTTATTTTTTACCTTCCCT294460.8810161455797948No Hit
CGAGGTTCTCGCAAGCTTACAAGCAGAGAAGGAGCAGAGTGTCCGCAATC221580.6629612087807204No Hit
CGAGGTTCTCGCAAGCTTTTCACATTTTTTATATCAAGAAATAAAGCAAG198440.5937269711636707No Hit
CGAGGTTCTCGCAAGCTTGTGCCACCATGCCCAGCCAATTTTGTGTGTGT169790.5080069665081619No Hit
CGAGGTTCTCGCAAGCTTACCTATTTTTAGGATCAATCAATGTGAGGAAT140830.42135945045847484No Hit
CGAGGTTCTCGCAAGCTTATCCACCAAGATCAATCAATGTGAGGAATGAC138980.415824301815798No Hit
CGAGGTTCTCGCAAGCTTGTAATCTCAGCTACTCAGGAGGCTGAGGCAGG110970.3320191593934315No Hit
CGAGGTTCTCGCAAGCTTCAGGAATACAAAGCTAAAAGACAAGGAATCCC104160.3116438284439022No Hit
CGAGGTTCTCGCAAGCTTTCACCTCTTGTTAAAGCAGTAAATGGTCTTTG97930.29300384139315805No Hit
CGAGGTTCTCGCAAGCTTCAGGAATACAAAGCTAAAGGACAAGGTATCCC74590.22317120932825135No Hit
CGAGGTTCTCGCAAGCTTCAGGAATACAAAGCTAAAAGGCAAGGTATCCC67980.20339427282657901No Hit
CGAGGTTCTCGCAAGCTTCAAAAATGCATAGAATTATGTAACCATTTCCA67450.2018085275397581No Hit
CGAGGTTCTCGCAAGCTTCTAGAAATAATAAGTGAGTTTAGCAAGTTAAC65890.19714105084647385No Hit
CGAGGTTCTCGCAAGCTTGTGGCCAAATGTAGACAAATTTTATGCATTAT65050.1946277941654746No Hit
CGAGGTTCTCGCAAGCTTCAGGAGTACAAAGCTAAAAGACAAGGTATCCC64840.19399947999522482No Hit
CGAGGTTCTCGCAAGCTTCAGGAATACGAAGCTAAAAGACAAGGTATCCC64190.19205469803968972No Hit
CGAGGTTCTCGCAAGCTTCAGGAATACAAAGCTAAAAGACGAGGTATCCC62730.18768641857033394No Hit
CGAGGTTCTCGCAAGCTTCAGGGATACAAAGCTAAAAGACAAGGTATCCC61440.1838267743816566No Hit
CGAGGTTCTCGCAAGCTTCAGGAATACAAAGCTAAAAGACAGGGTATCCC60330.18050568519605048No Hit
CGAGGTTCTCGCAAGCTTATCCACCATGATCAATCAATGTGAGGAATGAC59900.17921913713315804No Hit
CGAGGTTCTCGCAAGCTTCAGGAATACAAAGCTAAGAGACAAGGTATCCC57850.17308559404262425No Hit
CGAGGTTCTCGCAAGCTTCAGGAATACAAGGCTAAAAGACAAGGTATCCC57320.17149984875580332No Hit
CGAGGTTCTCGCAAGCTTCAGGAATACAAAGCTAAAAGACAAGGCATCCC57220.17120065153187483No Hit
CGAGGTTCTCGCAAGCTTCAGGAATACAGAGCTAAAAGACAAGGTATCCC56580.16928578929873256No Hit
CGAGGTTCTCGCAAGCTTGTGGGGGTTATATCCTACTGCTCAAGGTCATC55960.16743076651037603No Hit
CGAGGTTCTCGCAAGCTTCGGGAATACAAAGCTAAAAGACAAGGTATCCC55520.1661142987250907No Hit
CGAGGTTCTCGCAAGCTTGAGGAGCAAGGTAATAAAGTGGCACCTTAAAA55430.1658450212235551No Hit
CGAGGTTCTCGCAAGCTTAGCGTTCCAATAACGGAACACTAGGCATAATG55240.16527654649809098No Hit
CGAGGTTCTCGCAAGCTTTGGTGGCCGAAATTCTCGTATGCCGTCTTCTG55160.16503718871894818TruSeq Adapter, Index 19 (95% over 24bp)
CGAGGTTCTCGCAAGCTTCAGGAATACAAAGCTAAAAGACAAGGTACCCC53960.16144682203180646No Hit
CGAGGTTCTCGCAAGCTTTTTGATGTGCTGCTGGATTCGGTTTGCCAGTA53720.16072874869437812No Hit
CGAGGTTCTCGCAAGCTTCACGAATACAAAGCTAAAAGACAAGGTATCCC50250.15034660502405994No Hit
CGAGGTTCTCGCAAGCTTTTGTAGATGCCTTAAAGTTTTGTATGTAGACA49830.14908997668356033No Hit
CGAGGTTCTCGCAAGCTTTTTGAAAGATGGGATACCTTGTCTTTTAGCTT47450.1419690827540626No Hit
CGAGGTTCTCGCAAGCTTTCCTTGCTCCAGAGTGAGACACTATCTCCATC45320.135596181884386No Hit
CGAGGTTCTCGCAAGCTTCAGGAATACAAAGCTAGAAGACAAGGTATCCC42520.12721865961438864No Hit
CGAGGTTCTCGCAAGCTTTTACTCAAGGTACAGTTACATGATAAGCAAAA41300.1235684534824612No Hit
CGAGGTTCTCGCAAGCTTTCTGGAAAATTGATGAATATTTGAAAATTAAT38380.11483189454374967No Hit
CGAGGTTCTCGCAAGCTTCAGGAATGCAAAGCTAAAAGACAAGGTATCCC36600.10950618395782277No Hit
CGAGGTTCTCGCAAGCTTAGTTTGGCTGGATATGAAATTCTGGGTTGAAA36030.10780075978143046No Hit
CGAGGTTCTCGCAAGCTTCAGGAATACAAAGCTGAAAGACAAGGTATCCC35950.10756140200228767No Hit
CGAGGTTCTCGCAAGCTTGTTGTTTGTTTGTTTGATTCAGATGGGGGAAG34160.10220577169396791No Hit
CGAGGTTCTCGCAAGCTTCAGGAATACAAAGCCAAAAGACAAGGTATCCC33980.10166721669089666No Hit
CGAGGTTCTCGCAAGCTTCAGGAATACAAAGCTAAAAGACAAGGTGTCCC33960.10160737724611095No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)