FastQCFastQC Report
Sun 11 Sep 2022
EGAF00000710451

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000710451
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3008396
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTCAGGGAGTTAAGCTTCCCGTTTTTCTAGGTGAGTGTCCCAAAGTTA86488028.748874815682512No Hit
TTGTCAGGGAGTTAAGCTTCAGAACACCTTGGAATACAGTTTAGCTTCCT1114983.706227504623726No Hit
TTGTCAGGGAGTTAAGCTTGATTAGGTTAGGTATTTTTAAAGAAAACTCA356251.1841858585106482No Hit
TTGTCAGGGAGTTAAGCTTTCTTAGCACAGAAGGAGTAAACTGCAAATGA320691.0659833346407852No Hit
TTGTCAGGGAGTTAAGCTTGAGCTCCACCGAAACCAGCACGGAATTTCTA286760.9531989804533711No Hit
TTGTCAGGGAGTTAAGCTTGTTTTCAGCCAGTGTAAGCGTGGTGTTCTTG282090.9376757581116316No Hit
TTGTCAGGGAGTTAAGCTTTCCTAGGGTGCTACACAGATATATAAAGCTG228260.7587431973716227No Hit
TTGTCAGGGAGTTAAGCTTGCAAAAGGAGATCATAAGACAATTATTGGAT160340.5329750471679926No Hit
TTGTCAGGGAGTTAAGCTTATTTTAAAAAACGGATTATTGAAACCCAGAA149170.49584562670605864No Hit
TTGTCAGGGAGTTAAGCTTGTAAAGAATTTTGTGAGCTGAAGTATTTTAA135830.4515030601024599No Hit
TTGTCAGGGAGTTAAGCTTTGTGGAAAATGGTCTTTCAGCAGCAGCATCT132040.43890498458314664No Hit
TTGTCAGGGAGTTAAGCTTTGGAGGACAAGATGTGCTGATCATTATCTTA119670.39778672754517697No Hit
TTGTCAGGGAGTTAAGCTTTTATCACAATGAAAGAAATACTCAAAGCATG119310.3965900765723661No Hit
TTGTCAGGGAGTTAAGCTTTATGATATTGGGCAAGTGAATAACTTCTAGC112060.37249085559214945No Hit
TTGTCAGGGAGTTAAGCTTTACCTTTAAAGAGTCCGATGAACTAAAGGCA109610.3643469809160762No Hit
TTGTCAGGGAGTTAAGCTTTGAACCTAAGTTGAACCTTCCAACCCCGGCA95840.31857508120606465No Hit
TTGTCAGGGAGTTAAGCTTGTCTATGAACTGAAGGAAGAAAGATGGGACC95650.31794351541485893No Hit
TTGTCAGGGAGTTAAGCTTATACATAGTAGAGCAAGGAATTGAACCTAAA85650.28470321061456005No Hit
TTGTCAGGGAGTTAAGCTTAAGGATATAGCCATCCACTGTTAATTAACCT77450.25744616067831494No Hit
TTGTCAGGGAGTTAAGCTTCTCCACAGTGAAGGAAACAATAAACAGACTA76050.2527925180062731No Hit
TTGTCAGGGAGTTAAGCTTTATCCAAGCCTGAGGTTTAATCTTAACTACA70380.23394526518450365No Hit
TTGTCAGGGAGTTAAGCTTTAGTTGGCAAGGCAGTAGCAATTCTGGGTGC63490.2110426951770977No Hit
TTGTCAGGGAGTTAAGCTTGGATTATCCAGGAGGGAAACCTGACAATATA62720.2084831917074747No Hit
TTGTCAGGGAGTTAAGCTTCCCGTTTTTCTAGGGGAGTGTCCCAAAGTTA62660.2082837498786729No Hit
TTGTCAGGGAGTTAAGCTTCACTCATTTTTTCAACCAGTTGGCTGCAATT62360.2072865407346639No Hit
TTGTCAGGGAGTTAAGCTTGGGCCTGTGATGTTCATCTCAGCCCCATGTC61000.20276585928182328No Hit
TTGTCAGGGAGTTAAGCTTAATCAGGCAGGACATTAGAGTCCAGGGGGGC59500.19777981356177843No Hit
TTGTCAGGGAGTTAAGCTTCCAACTAATGAGAATGAATCTCATCAATGTC57490.19109851229691835No Hit
TTGTCAGGGAGTTAAGCTTATCCACCAGGATCAAGTGGGCTTCATCCCTG57210.19016778376251No Hit
TTGTCAGGGAGTTAAGCTTTAGAAAACTCTTTTTTCTTATCAGAATCTAG56120.1865445905392774No Hit
TTGTCAGGGAGTTAAGCTTCTTGCAGCACCTTTTTTTCACTGTCGCATCA54100.17983004896961705No Hit
TTGTCAGGGAGTTAAGCTTCAGGCATCACGATATTCCCATATGTCTACTG54090.17979680866481673No Hit
TTGTCAGGGAGTTAAGCTTCTGGAACCTAACCCTGCTGTAAGCCTCACCC52780.17544232873597757No Hit
TTGTCAGGGAGTTAAGCTTATGAATACCCCAGTTGAAAAACAAATGGCTT50970.16942583356712348No Hit
TTGTCAGGGAGTTAAGCTTCATAAAAGTATAAGCAATTCCAGATTCAAAA50270.16709901223110254No Hit
TTGTCAGGGAGTTAAGCTTTGGAGTCATATCTGGGCCTGTATCTGTCTCT49770.1654369969910876No Hit
TTGTCAGGGAGTTAAGCTTAATGCAGAAAATATACCCAAGTCAGGAGGTA49740.16533727607668672No Hit
TTGTCAGGGAGTTAAGCTTCATAAGTGAAGGAGAAATAAAATACTTTACA49590.16483867150468223No Hit
TTGTCAGGGAGTTAAGCTTTGAGGTCTGCACATCAGATAGCATTACTCCC49340.16400766388467475No Hit
TTGTCAGGGAGTTAAGCTTTGTTGGGAGGATGCAGTGAGGTGATATATGG47530.15799116871582067No Hit
TTGTCAGGGAGTTAAGCTTTCACTTGGGGATGTGGGCTTTGGTCGAAACT47090.1565285953046075No Hit
TTGTCAGGGAGTTAAGCTTCCTCTTGGGAGAATTAGGGAAGTTTCAAGGC47010.15626267286620513No Hit
TTGTCAGGGAGTTAAGCTTATTTTGAGGGTAGCAACATGTCATATGTAAG46770.15546490555099793No Hit
TTGTCAGGGAGTTAAGCTTATCCCTTTTCCCTGAAAAACCTCTGTTGTAC46270.153802890310983No Hit
TTGTCAGGGAGTTAAGCTTACCTCATTTTGGTTTTGTCAATAAAGGGTCA45880.15250651842377133No Hit
TTGTCAGGGAGTTAAGCTTAATTTTTCCAACAGCCCTGCTCATAAAAGGA45810.15227383629016925No Hit
TTGTCAGGGAGTTAAGCTTTGATTTTTGCAGCTCCCCTGAAGATTTGGAA45320.1506450613549546No Hit
TTGTCAGGGAGTTAAGCTTTTTTATTTAAAAAAACCCAATAACTCAGTTT45180.15017969708775042No Hit
TTGTCAGGGAGTTAAGCTTGTATTGTTTCTTGGACCCATCCCCTACACTC44550.1480855578853316No Hit
TTGTCAGGGAGTTAAGCTTTTCTGCCAGACTACTCAGAAGTAAAGTGATT44390.1475537130085268No Hit
TTGTCAGGGAGTTAAGCTTTAATCAGTAATCTTCAAGTAGGAAGTTATTC44190.14688890691252082No Hit
TTGTCAGGGAGTTAAGCTTCTTTTAAGAAATCAGACTGTTTATACCCCAA43570.1448280080149023No Hit
TTGTCAGGGAGTTAAGCTTCTGCACTTTCAACAAAACTGTAACCAGGGGC42740.14206906271647748No Hit
TTGTCAGGGAGTTAAGCTTTTTTTATTTAAAAAAACCCAATAACTCAGTT42300.14060648930526434No Hit
TTGTCAGGGAGTTAAGCTTTATACCAGACGAAGTAAATAGAAAAGAAGAA41790.1389112337604491No Hit
TTGTCAGGGAGTTAAGCTTCCCGTTTTTCTAGGTGGGTGTCCCAAAGTTA41220.13701653638683203No Hit
TTGTCAGGGAGTTAAGCTTCCCGTTTTTCTAGGCGAGTGTCCCAAAGTTA41220.13701653638683203No Hit
TTGTCAGGGAGTTAAGCTTGATTATGGGTGATGCTAGGCCCAATTTTCAC41170.13685033486283055No Hit
TTGTCAGGGAGTTAAGCTTCTGAAAGATCAAAAGAGGTCAGATGTACAGA39930.1327285370675935No Hit
TTGTCAGGGAGTTAAGCTTCTGAAACACCTTATTTCAGCTTATCTTCAAT39840.1324293743243908No Hit
TTGTCAGGGAGTTAAGCTTAACAGGACACATAGGAAAAGTCCATAATTTG38870.1292050647587618No Hit
TTGTCAGGGAGTTAAGCTTCCCGTTTTTCTAGGTGAGTGTCCCAAGGTTA38370.12754304951874687No Hit
TTGTCAGGGAGTTAAGCTTCAATTATAAAATGAATGACAGATGCACAAGA37230.12375365477151279No Hit
TTGTCAGGGAGTTAAGCTTTTAACATAGGGAGCCCCTTATTCACCATAAG37050.12315532928510742No Hit
TTGTCAGGGAGTTAAGCTTTTTAGCTGAAGGAGGCTTTGAGCCAAATTTA36880.12259024410350232No Hit
TTGTCAGGGAGTTAAGCTTGATGCAGTGGTTCACACCTGTAATCCCATCA36740.12212487983629815No Hit
TTGTCAGGGAGTTAAGCTTTTTCCTTCGCAAGTACTGCTACATCTACATC36470.12122739160669008No Hit
TTGTCAGGGAGTTAAGCTTGCTATGATCTCTAGAGAGCATATTTGCTATG35160.11687291167785092No Hit
TTGTCAGGGAGTTAAGCTTTCTTTGCCTTCCTCTCATTGAGCATTTGATT34140.11348240058822044No Hit
TTGTCAGGGAGTTAAGCTTCTATTTTCGCTATTTGAATGAGTGATGTTGG33940.11281759449221446No Hit
TTGTCAGGGAGTTAAGCTTACTAAATTTTTCAGTTAATGACGGTGGCATT33590.111654183824204No Hit
TTGTCAGGGAGTTAAGCTTTACAGTGGCTCCATTTCACTCAGGCTCACCA33260.11055725376579413No Hit
TTGTCAGGGAGTTAAGCTTCCCGTTTTTCTAGGTGAGTGCCCCAAAGTTA32320.10743266511456603No Hit
TTGTCAGGGAGTTAAGCTTAGAGGACATTGTGGAGATGTTAACTGGCCTA31990.10633573505615618No Hit
TTGTCAGGGAGTTAAGCTTCCCGTTCTTCTAGGTGAGTGTCCCAAAGTTA31700.10537176621694751No Hit
TTGTCAGGGAGTTAAGCTTCCAAATCAGGTGACTTTCAGTTAATAAAAGA31630.1051390840833454No Hit
TTGTCAGGGAGTTAAGCTTCACAGCTGCTGATGGACACAGACACTGGGAC31610.10507260347374481No Hit
TTGTCAGGGAGTTAAGCTTCCCGCTTTTCTAGGTGAGTGTCCCAAAGTTA31390.10434131676813824No Hit
TTGTCAGGGAGTTAAGCTTTCAAACAGAATTTAAAGAGTTAACGCTGGCC30950.10287874335692508No Hit
TTGTCAGGGAGTTAAGCTTCTCTGTTCCCTGATGCAGTGTGGATATTTTT30790.10234689848012031No Hit
TTGTCAGGGAGTTAAGCTTCCCGTTTTTCTAGGTGAGCGTCCCAAAGTTA30720.10211421634651822No Hit
TTGTCAGGGAGTTAAGCTTTTTAGCTTGATGTAGTCTCATTTGTCTATTT30270.10061840263050476No Hit
TTGTCAGGGAGTTAAGCTTCCCGTTTTTCTAGGTGAGTGTCCCAAAGCTA30120.10011979805850028No Hit
TTGTCAGGGAGTTAAGCTTCCCGTTTCTCTAGGTGAGTGTCCCAAAGTTA30110.10008655775369998No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)