FastQCFastQC Report
Sun 11 Sep 2022
EGAF00000710452

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000710452
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2914547
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAACCTAATCAAGCTTGAGCTCCACCGAAACCAGCACGGAATTTCTA31030010.646594479347906No Hit
ACCTAACCTAATCAAGCTTGTAAAGAATTTTGTGAGCTGAAGTATTTTAA1506305.168213104815259No Hit
ACCTAACCTAATCAAGCTTTTATCACAATGAAAGAAATACTCAAAGCATG1048513.5975058902807193No Hit
ACCTAACCTAATCAAGCTTTCCTAGGGTGCTACACAGATATATAAAGCTG909183.119455613513867No Hit
ACCTAACCTAATCAAGCTTCCCGTTTTTCTAGGTGAGTGTCCCAAAGTTA856292.9379865893396127No Hit
ACCTAACCTAATCAAGCTTAACTCCCTGACAAAACTAGGAATTCCCTGAA381221.3079905728059968No Hit
ACCTAACCTAATCAAGCTTTTCTGCTTCTTAGGGGGCTTTAATCTAAAGA234970.80619732672007No Hit
ACCTAACCTAATCAAGCTTTGGAGGACAAGATGTGCTGATCCCTATTTTA220480.7564811958770952No Hit
ACCTAACCTAATCAAGCTTTGGAGTCATATCTGGGCCTGTATCTGTCTCT147420.5058075920546143No Hit
ACCTAACCTAATCAAGCTTAATCAGGCAGGACATTAGAGTCCAGGGGGGC146660.5031999827074327No Hit
ACCTAACCTAATCAAGCTTCTTGCAGCACCTTTTTTTCACTGTCGCATCA146530.5027539442664675No Hit
ACCTAACCTAATCAAGCTTGCAAAAGGAGATCCCTATTTTACTTTTACGT144490.4957545718082433No Hit
ACCTAACCTAATCAAGCTTATTTTGAGGGTAGCAACATGGCGTTCCCAAC136150.46713949028785606No Hit
ACCTAACCTAATCAAGCTTATACATAGTAGAGCAAGGAATTGAACCTAAA134260.46065477756920714No Hit
ACCTAACCTAATCAAGCTTCTCCACAGTGAAGGAAACAATAAACAGACTA131370.4507389999200562No Hit
ACCTAACCTAATCAAGCTTGTTTTCAGCCAGTGTAAGCGTGGTGTTCTTG121820.4179723298337615No Hit
ACCTAACCTAATCAAGCTTTGAACCTAAGTTGAACCTTCCAACCCCGGCA113530.3895288015598994No Hit
ACCTAACCTAATCAAGCTTCCAACTAATGAGAATGAATCTCATCAATGTC110340.3785837044315978No Hit
ACCTAACCTAATCAAGCTTGAGTCTGGGGAGAAGGATGGTGGGAAAGAAA110200.3781033553413275No Hit
ACCTAACCTAATCAAGCTTTGAGGTCTGCACATCAGATAGCATTACTCCC106160.36424185302209916No Hit
ACCTAACCTAATCAAGCTTGATGCAGTGGTTCACACCTGTAATCCCATCA104560.35875214913329584No Hit
ACCTAACCTAATCAAGCTTTCATGCTATGTTCAGTAAGGCAGAATCAAAT100500.3448220255154575No Hit
ACCTAACCTAATCAAGCTTTTGCTTTCACTCCAATTAAAACAACAATGGT96550.3312693190399743No Hit
ACCTAACCTAATCAAGCTTGATCCCTATTTTACTTTTACGTGCTTAATCC92930.3188488639915568No Hit
ACCTAACCTAATCAAGCTTAGCAACATATTCTGAGTCATCATGGGAGAAT90660.31106034659931714No Hit
ACCTAACCTAATCAAGCTTCACAGCTGCTGATGGACACAGACACTGGGAC89350.3065656515403594No Hit
ACCTAACCTAATCAAGCTTCTCGCACTAAACAGCCAAGTTGTGAATGCAA84130.28865549260313866No Hit
ACCTAACCTAATCAAGCTTTACAGTGGCTCCATTTCACTCAGGCTCACCA83030.28488132117958637No Hit
ACCTAACCTAATCAAGCTTTAGAAAACTCTTTTTTCTTATCAGAATCTAG83010.28481269988097635No Hit
ACCTAACCTAATCAAGCTTAGTTTGGCTGGATATGAAATTCTGGGTTGAA82720.28381769105113075No Hit
ACCTAACCTAATCAAGCTTAGTCATCTGACTTAGAAAGGACAGAAACTAT81170.27849954040885255No Hit
ACCTAACCTAATCAAGCTTTGATTTTTGCAGCTCCCCTGAAGATTTGGAA79860.2740048453498949No Hit
ACCTAACCTAATCAAGCTTCAATTATAAAATGAATGACAGATGCACAAGA77280.26515269782919954No Hit
ACCTAACCTAATCAAGCTTCCAAATCAGGTGACTTTCAGTTAATAAAAGA73900.2535556983641025No Hit
ACCTAACCTAATCAAGCTTCCTAAATCATTAAAAGGATATCCTTTTAAAG73280.2514284381071913No Hit
ACCTAACCTAATCAAGCTTCAACTACGAATAAATAGCACCCATCTGCTTG72990.2504334292773457No Hit
ACCTAACCTAATCAAGCTTATCCACCATGATCCCTATTTTACTTTTACGT71890.24665925785379342No Hit
ACCTAACCTAATCAAGCTTCCAGCACCAGACAGGGAAGAAGGAGTTCTGA68150.23382707501371566No Hit
ACCTAACCTAATCAAGCTTACCTCATTTTGGTTTTGTCAATAAAGGGTCA67710.23231740644429477No Hit
ACCTAACCTAATCAAGCTTTATACCAGATGAAGTAAATAGAAAAGAAGAA67580.2318713680033295No Hit
ACCTAACCTAATCAAGCTTCAATATCTGAGGGCAGGAGAAGGTAGATATC64240.22041161113545263No Hit
ACCTAACCTAATCAAGCTTTTTTATTTAAAAAAACCCAATAACTCAGTTT63450.21770106984035598No Hit
ACCTAACCTAATCAAGCTTGATTATGGGTGATGCTAGGCCCAATTTTCAC62930.21591691607649488No Hit
ACCTAACCTAATCAAGCTTTTCTTTCAAGGGTGAATAATATTAATACTTG62540.21457880075359909No Hit
ACCTAACCTAATCAAGCTTCATAAGTGAAGGAGAAATAAAATACTTTACA60910.20898616491688074No Hit
ACCTAACCTAATCAAGCTTTCTTAGCACAGAAGGAGTAAACTGCAAATGA60320.20696183660788453No Hit
ACCTAACCTAATCAAGCTTGTTTTGAAGTCTAAAGGCCCAATATGTCTCA59280.2033935290801624No Hit
ACCTAACCTAATCAAGCTTGTCCACTTAATTACAGAGAAGGAAAAACTAA58020.19907038726772974No Hit
ACCTAACCTAATCAAGCTTCCTCTTGGGAGAATTAGGGAAGTTTCAAGGC55550.19059565688938968No Hit
ACCTAACCTAATCAAGCTTTCTATTTTGTTAAATCTTTTTATGCTTGGCT53560.18376783767769056No Hit
ACCTAACCTAATCAAGCTTTAGTGTGCTCAGAATGACCAGAGAACCTTGT52480.18006228755274833No Hit
ACCTAACCTAATCAAGCTTAATGCAGAAAATATACCCAAGTCAGGAGGTA52240.17923883196942783No Hit
ACCTAACCTAATCAAGCTTGAGAAAGTTTCTGCATTCCCTTGTTAATTTC50690.17392068132714963No Hit
ACCTAACCTAATCAAGCTTCTCTGTTCCCTGATGCAGTGTGGATATTTTT50270.1724796340563388No Hit
ACCTAACCTAATCAAGCTTGCCTGGTTCTTAGGCCTTCAGATCCCTATTT49450.16966616081332708No Hit
ACCTAACCTAATCAAGCTTCAGAACACCTTGGAATACAGTTTAGCTTCCT47220.1620148860183075No Hit
ACCTAACCTAATCAAGCTTTTTTTATTTAAAAAAACCCAATAACTCAGTT47140.16174040082386731No Hit
ACCTAACCTAATCAAGCTTAGTCCTGAGACCCGGGGAGATTAAAAAAAAA45850.15731432706351964No Hit
ACCTAACCTAATCAAGCTTATCCACCACGATCCCTATTTTACTTTTACGT44260.15185893382402138No Hit
ACCTAACCTAATCAAGCTTCGTCTTCATGAAACTTATATTTTAGAAGGGG44060.15117272083792097No Hit
ACCTAACCTAATCAAGCTTAAAGTGTCAATTTTTATACTTATCTGGTTTG43290.14853080084143438No Hit
ACCTAACCTAATCAAGCTTATTTGAGCCTGTTGGGGTGAGGTGGGTGCAA41930.14386455253595157No Hit
ACCTAACCTAATCAAGCTTACCCAGTGTGTTAGGCATAATACTAGCCCTC41690.14304109695263106No Hit
ACCTAACCTAATCAAGCTTAGGTTCAGGCAATCTTCAGAATCAAGAAGAC41500.14238919461583568No Hit
ACCTAACCTAATCAAGCTTCATAAAAGTATAAGCAATTCCAGATTCAAAA41230.14146280708460013No Hit
ACCTAACCTAATCAAGCTTCATAAGTGAAGGAGAAATAAAATCCTTTACA40720.13971296397004407No Hit
ACCTAACCTAATCAAGCTTTAAGCCAAGCAAAAGAGTTTGGATTTTTTCC40550.1391296829318587No Hit
ACCTAACCTAATCAAGCTTGGGCCTGTGATGTTCATCTCAGCCCCATGTG40530.13906106163324866No Hit
ACCTAACCTAATCAAGCTTCCCAGTCACTTTGTTTACGTACTCAAGCCTC39080.13408601748402066No Hit
ACCTAACCTAATCAAGCTTTTACATAGCTCAGAATAATTTTTATCATTAC38030.1304833993069935No Hit
ACCTAACCTAATCAAGCTTTTTTCTATTAAATTTTTTTTATTACTTTTAA37420.12839044969938726No Hit
ACCTAACCTAATCAAGCTTAGTTTGTCTGGATATGAAATTCTGGGTTGAA37340.12811596450494708No Hit
ACCTAACCTAATCAAGCTTCTTTTAAGAAATCAGACTGTTTATACCCCAA36560.12543973385915547No Hit
ACCTAACCTAATCAAGCTTTATACCAGACGAAGTAAATAGAAAAGAAGAA36310.12458196762652997No Hit
ACCTAACCTAATCAAGCTTTCAAAAGTAGGAAGCTGGTCAATGAGAATTT36060.12372420139390443No Hit
ACCTAACCTAATCAAGCTTACTGTCCCATGAATTATCATAATATGACAAA35780.12276350321336385No Hit
ACCTAACCTAATCAAGCTTCTGACTTAAGTGCCCTGAAAAGGCTTTATGG35250.12094503880019777No Hit
ACCTAACCTAATCAAGCTTCTGGACAGCAAAAGAAATAATCATCAGAGTA33850.11614154789749487No Hit
ACCTAACCTAATCAAGCTTCACGGTGCAGTACATTTCCATATGGACAGGG33510.11497498582112417No Hit
ACCTAACCTAATCAAGCTTGCATGTCTGCCTTCTTCCTCATAGAAACACT33460.11480343257459907No Hit
ACCTAACCTAATCAAGCTTTTCTGTTATCCTGACTAGAACTTACCATCTT33050.11339669595309324No Hit
ACCTAACCTAATCAAGCTTGCCAACCTCTCCTCTAATCTAGTCTGCCTTG31590.1083873411545602No Hit
ACCTAACCTAATCAAGCTTATCCACCAGGATCCCTATTTTACTTTTACGT31450.10790699206428991No Hit
ACCTAACCTAATCAAGCTTCTACCACCTCTTCTGGAATGGTTCTAGCTAG31240.10718646842888449No Hit
ACCTAACCTAATCAAGCTTTATGAAAAAGAGGCCAGAGGAGGGAGACTGG31200.1070492258316644No Hit
ACCTAACCTAATCAAGCTTTCTAGTCTTTCTCAAGGAAACCTGTCCAAAG30810.10571111050876861No Hit
ACCTAACCTAATCAAGCTTTACAATACCCTGATATATAGGAATTATACTA30740.10547093596363347No Hit
ACCTAACCTAATCAAGCTTCAAATGGGAAGGATCCCTATTTTACTTTTAC30640.10512782947058326No Hit
ACCTAACCTAATCAAGCTTAGCAAGAATTTTAAATTACTGAGTAAATGAA30610.10502489752266819No Hit
ACCTAACCTAATCAAGCTTTAAACCTTTTGGAATGTAAAGGCATATTTGT30160.10348091830394227No Hit
ACCTAACCTAATCAAGCTTTTTAGAAAGAAAAACCATACAGCAGTATGGT29990.10289763726575692No Hit
ACCTAACCTAATCAAGCTTTTTGATGTGCTGCTGGATTCGGTTTGCCAGT29940.10272608401923179No Hit
ACCTAACCTAATCAAGCTTGGATTATCCAGGAGGGAAACCTGACAATATA29860.10245159882479164No Hit
ACCTAACCTAATCAAGCTTGAACAAAGCACAGTGGATATTGAAAGAAAGA29830.10234866687687658No Hit
ACCTAACCTAATCAAGCTTACTAAATTTTTCAGTTAATGATGGTGGCATT29560.101422279345641No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)